Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	333,098	T→C	intergenic (‑65738/+11507)	unknown ← / ← unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Arg‑ACG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	333,098	0	T	C	100.0%	93.1 / NA	34	intergenic (‑65738/+11507)	unknown/unknown	SSU rRNA ## 16S rRNA, small subunit ribosomal RNA/tRNA‑Arg‑ACG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/13);  total (21/13)

AATGTGAGATTATCGCCGTAAAAACGTTGGAAAAAAACACCGCACTTTTGGGGAAAGTGCGGTGGGATTTTAAATTGTTTTTTGATAGTGTCGACTAGAGAGGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACTATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTCACTTCTTAGTGAATAAGCAATTTCCCAAGGTATCCATTGATCTTTCTCTGATTTCCAAGAATTTTTTATA  >  1/332862‑333336                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               aaTGTGAGATTATCGCCGTAAAAACGTTGGAAAAAAACACCGCACTTTTGGGGAAAGTGCGGTGGGATTTTAAATTGTTTTTTGATAGTGTCGACTAGAGAGGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTtat                                                                                                                                                                                                                                  >  1:467283/1‑251 (MQ=255)                  gTAAAAACGTTGGAAAAAAACACCGCACTTTTGGGGAAAGTGCGGTGGGATTTTAAATTGTTTTTTGATAGTGTCGACTAGAGAGGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTa                                                                                                                                                                                                                 >  1:721616/1‑251 (MQ=255)                        aCGTTGGAAAAAAACACCGCACTTTTGGGGAAAGTGCGGTGGGATTTTAAATTGTTTTTTGATAGTGTCGACTAGAGAGGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAgg                                                                                                                                                                                                           <  1:713793/251‑1 (MQ=255)                                                                     tttAAATTGTTTTTTGATAGTGTCGACTAGAGAGGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGaa                                                                                                                                                              <  1:658457/251‑1 (MQ=255)                                                                             gTTTTTTGATAGTGTCGACTAGAGAGGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGgtg                                                                                                                                                                                                                   <  1:308614/190‑1 (MQ=255)                                                                                        gtgtCGACTAGAGAGGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGttt                                                                                                                                                                                                                                    >  1:432969/1‑162 (MQ=255)                                                                                          gtCGACTAGAGAGGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCa                                                                                                                                                                                                             >  1:687599/1‑183 (MQ=255)                                                                                            cGACTAGAGAGGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCata                                                                                                                                       >  1:648272/1‑251 (MQ=255)                                                                                                   agagGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCAtatctatc                                                                                                                                 <  1:578159/250‑1 (MQ=255)                                                                                                                  gTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAAt                                                                                                                 >  1:163921/1‑251 (MQ=255)                                                                                                                      tattaCAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATgtagta                                                                                                                                                                                                                          <  1:831146/142‑1 (MQ=255)                                                                                                                             atatTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACg                                                                                                                       >  1:151500/1‑234 (MQ=255)                                                                                                                                ttatAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAg                                                                                                                                                                                                  >  1:204832/1‑156 (MQ=255)                                                                                                                                                          ttCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTGCGTAATCATAATAACCATATCTATCAGGt                                                                                                                             >  1:455996/1‑199 (MQ=255)                                                                                                                                                                tGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTTActtc                                                                   <  1:505853/251‑1 (MQ=255)                                                                                                                                                                    gCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGc                                                                                                                                                                                                              >  1:636715/1‑108 (MQ=255)                                                                                                                                                                    gCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGc                                                                                                                                                                      >  1:7785/1‑148 (MQ=255)                                                                                                                                                                                gCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGCAATCATAATAACCAtatctatc                                                                                                                                 <  1:197783/173‑1 (MQ=255)                                                                                                                                                                                            gagaTAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGgtg                                                                                                                                                                                                                   >  1:892597/1‑79 (MQ=255)                                                                                                                                                                                            gagaTAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGtt                                                                                                                                                                                                         >  1:721905/1‑89 (MQ=255)                                                                                                                                                                                            gagaTAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTTACTTCTTAGTGAATAAGCAATTTCCCAAgg                                          >  1:227813/1‑248 (MQ=255)                                                                                                                                                                                                tAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTTACTTCTTAGTGAATAAGCAATTTCCCAAGGTATCCAt                                   <  1:498206/251‑1 (MQ=255)                                                                                                                                                                                                   aCTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAg                                                                                                                           >  1:171029/1‑160 (MQ=255)                                                                                                                                                                                                   aCTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTTACTTCTTAGTGAATAAGCAATTTCCCAAGGTATCCATTGa                                <  1:573053/251‑1 (MQ=255)                                                                                                                                                                                                    cTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATg                                                                                                                                                                                                                               <  1:899170/59‑1 (MQ=255)                                                                                                                                                                                                    cTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTTACTTCTTAGTGAATAAGCAATTTCCCAAGGTATCCATTGAt                               <  1:504667/251‑1 (MQ=255)                                                                                                                                                                                                           cAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCataa                                                                                                                                               <  1:99756/132‑1 (MQ=255)                                                                                                                                                                                                           cAGTTACATTGAAGCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTTACTTCTTAGTGAATAAGCAATTTCCCAAgg                                          >  1:541095/1‑233 (MQ=255)                                                                                                                                                                                                                        gCATATATGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTTACTTCTTAGTGAATAAGCAATTTCCCAAGGTATCCATTGATCTTTCTCTGATTTCCAAGaa           >  1:802729/1‑251 (MQ=255)                                                                                                                                                                                                                          atatatGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTTACTTCTTAGTGAATAAGCAATTTCCCAAGGTATCCATTGATCTTTCTCTGATTTCCAAGAAtt         >  1:511625/1‑251 (MQ=255)                                                                                                                                                                                                                           tatatGAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGgtg                                                                                                                                                                                                                   >  1:61971/1‑48 (MQ=255)                                                                                                                                                                                                                                gAATTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTTAct                                                                     <  1:491308/185‑1 (MQ=255)                                                                                                                                                                                                                                 aaTTAGAACAACCATTCCTTAGTTTATATGTAGTATATGgtg                                                                                                                                                                                                                   >  1:141918/1‑42 (MQ=255)                                                                                                                                                                                                                                 aaTTAGAACAACCATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTTACTTCTTAGTGAATAAGCAATTTCCCAAGGTATCCATTGATCTTTCTCTGATTTCCAAGAATTTTTtata  >  1:322967/1‑251 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               AATGTGAGATTATCGCCGTAAAAACGTTGGAAAAAAACACCGCACTTTTGGGGAAAGTGCGGTGGGATTTTAAATTGTTTTTTGATAGTGTCGACTAGAGAGGGAAGTTTATGGTCTTATTACAATATTATAATTATTTCCTTTATCTCTAATTTCTATTGCAGCTTCAATCCAAGCATTCGGATTAGAGATAAACTTATCCCAGTTACATTGAAGCATATATGAATTAGAACAACTATTCCTTAGTTTATATGTAGTATATGGTGTATGCAGGTTATTCAAGTTTCTTTGAATAATATCAAAATAAAAGCCATATGAATTTACGTAATCATAATAACCATATCTATCAGGTAGAACGACCAATAAAATTGCATTACGCTTGCTTCGGTTATCTGGACGTCGCTCACTTCTTAGTGAATAAGCAATTTCCCAAGGTATCCATTGATCTTTCTCTGATTTCCAAGAATTTTTTATA  >  1/332862‑333336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑