Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,518,287	T→C	intergenic (‑73659/‑123477)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,518,287	0	T	C	100.0%	223.6 / NA	78	intergenic (‑73659/‑123477)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/52);  total (26/52)

ACGGTAAGCGCCGTGCCGCCGCCGTTTTTGCCGCCGATATTGCGTTCCAGTAACGGTTTCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGTACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGTATAATCTGCGCGATATATAATTT  >  1/1518055‑1518493                                                                                                                                                                                                                                         |                                                                                                                                                                                                               aCGGTAAGCGCCGTGCCGCCGCCGTTTTTGCCGCCGATATTGCGTTCCAGTAACGGTTTCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCCGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTc                                                                                                                                                                                              <  1:367901/251‑1 (MQ=255)                       cgTTTTTGCCGCCGATATTGCGTTCCAGTAACGGTTTCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAat                                                                                                                                                                        <  1:201932/251‑1 (MQ=255)                         tttttGCCGCCGATATTGCGTTCCAGTAACGGTTTCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAatat                                                                                                                                                                      <  1:615051/251‑1 (MQ=255)                         tttttGCCGCCGATATTGCGTTCCAGTAACGGTTTCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAatat                                                                                                                                                                      <  1:715644/251‑1 (MQ=255)                               ccgccgATATTGCGTTCCAGTAACGGTTTCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGt                                                                                                                                                                 <  1:338136/250‑1 (MQ=255)                                  ccgATATTGCGTTCCAGTAACGGTTTCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTa                                                                                                                                                             >  1:709985/1‑251 (MQ=255)                                                   tAACGGTTTCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCgg                                                                                                                                             <  1:659777/250‑1 (MQ=255)                                                      cGGTTTCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGc                                                                                                                                         <  1:819983/251‑1 (MQ=255)                                                          ttCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCAcat                                                                                                                                     <  1:393154/251‑1 (MQ=255)                                                          ttCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCAcat                                                                                                                                     <  1:646735/251‑1 (MQ=255)                                                              ggCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCgg                                                                                                                                             >  1:842204/1‑239 (MQ=255)                                                              ggCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCAcatcat                                                                                                                                  <  1:874449/250‑1 (MQ=255)                                                                cTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTTGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATAc                                                                                                                                <  1:807315/250‑1 (MQ=255)                                                                  gATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAAtt                                                                                                                                                                                   >  1:418446/1‑197 (MQ=255)                                                                  gATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAatat                                                                                                                                                                      <  1:71246/210‑1 (MQ=255)                                                                  gATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAat                                                                                                                                                                        <  1:403271/208‑1 (MQ=255)                                                                  gATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATc                                                                                                                                                                   >  1:285410/1‑213 (MQ=255)                                                                  gATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGAc                                                                                                                                                         <  1:160796/223‑1 (MQ=255)                                                                  gATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACtat                                                                                                                             >  1:365516/1‑251 (MQ=255)                                                                  gATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACtat                                                                                                                             >  1:527342/1‑251 (MQ=255)                                                                   aTTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAatatat                                                                                                                                                                    >  1:866659/1‑211 (MQ=255)                                                                   aTTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAatat                                                                                                                                                                      >  1:497792/1‑209 (MQ=255)                                                                   aTTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACtata                                                                                                                            >  1:354361/1‑251 (MQ=255)                                                                   aTTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACtat                                                                                                                             <  1:720434/250‑1 (MQ=255)                                                                   aTTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACtat                                                                                                                             <  1:122491/250‑1 (MQ=255)                                                                   aTTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACtat                                                                                                                             <  1:468673/250‑1 (MQ=255)                                                                    ttttATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTatat                                                                                                                           <  1:569708/251‑1 (MQ=255)                                                                    ttttATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTatat                                                                                                                           <  1:534900/251‑1 (MQ=255)                                                                    ttttATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTatat                                                                                                                           <  1:419142/251‑1 (MQ=255)                                                                         ttCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGt                                                                                                                      <  1:675523/251‑1 (MQ=255)                                                                               gCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGttt                                                                                                                                                               >  1:293798/1‑204 (MQ=255)                                                                               gCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCAcat                                                                                                                                     >  1:279872/1‑230 (MQ=255)                                                                                cTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAAt                                                                                                               <  1:389199/251‑1 (MQ=255)                                                                                         gATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTatat                                                                                                                           <  1:403316/230‑1 (MQ=255)                                                                                           tCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACtat                                                                                                                             >  1:512122/1‑226 (MQ=255)                                                                                                    cTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGt                                                                                                                      >  1:675990/1‑224 (MQ=255)                                                                                                    cTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGaa                                                                                                          >  1:79439/1‑236 (MQ=255)                                                                                                    cTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGtt                                                                                           >  1:633813/1‑251 (MQ=255)                                                                                                    cTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTAGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGaa                                                                                                          >  1:449669/1‑236 (MQ=255)                                                                                                         gTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAg                                                                                                            <  1:64018/229‑1 (MQ=255)                                                                                                            aCTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACAt                                                                                   >  1:495053/1‑251 (MQ=255)                                                                                                             cTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATa                                                                                  >  1:522123/1‑251 (MQ=255)                                                                                                                 cTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCgg                                                                                                                                             >  1:745731/1‑188 (MQ=255)                                                                                                                    tGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATc                                                                           <  1:324397/251‑1 (MQ=255)                                                                                                                          tttttGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGa                                                                     <  1:595979/251‑1 (MQ=255)                                                                                                                          tttttGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGa                                                                     <  1:124664/251‑1 (MQ=255)                                                                                                                            tttGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATc                                                                   <  1:616782/251‑1 (MQ=255)                                                                                                                            tttGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATc                                                                   <  1:300327/251‑1 (MQ=255)                                                                                                                                    ttGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTg                                                           <  1:710121/251‑1 (MQ=255)                                                                                                                                    ttGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTg                                                           <  1:117293/251‑1 (MQ=255)                                                                                                                                     tGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCg                                                                                                                                              >  1:851454/1‑167 (MQ=255)                                                                                                                                      ggTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAatat                                                                                                                                                                      <  1:58919/142‑1 (MQ=255)                                                                                                                                      ggTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAatat                                                                                                                                                                      <  1:19139/142‑1 (MQ=255)                                                                                                                                      ggTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACtat                                                                                                                             >  1:191325/1‑183 (MQ=255)                                                                                                                                      ggTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTatat                                                                                                                           <  1:467293/185‑1 (MQ=255)                                                                                                                                      ggTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAAt                                                                                               <  1:808897/213‑1 (MQ=255)                                                                                                                                      ggTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGtt                                                         >  1:803657/1‑251 (MQ=255)                                                                                                                                       gTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACtat                                                                                                                             >  1:198504/1‑182 (MQ=255)                                                                                                                                          aaTGGAGCCGCATTTTTCAATTTCTTTTAATTAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCt                                                             >  1:229286/1‑243 (MQ=255)                                                                                                                                            tGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCaaaaa                                                   <  1:772713/251‑1 (MQ=255)                                                                                                                                                        tttCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAatatat                                                                                                                                                                    <  1:635633/126‑1 (MQ=255)                                                                                                                                                        tttCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGt                                                                                                                                                                 <  1:796430/129‑1 (MQ=255)                                                                                                                                                         ttCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTg                                      <  1:78648/251‑1 (MQ=255)                                                                                                                                                            aaTTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTgcgc                                   <  1:517917/251‑1 (MQ=255)                                                                                                                                                                tCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCgg                                                                                                                                             <  1:156411/141‑1 (MQ=255)                                                                                                                                                                      aataaACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGt                         <  1:686345/251‑1 (MQ=255)                                                                                                                                                                        taaACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGtat                       <  1:901144/251‑1 (MQ=255)                                                                                                                                                                          aaCGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGTATaa                     <  1:143890/251‑1 (MQ=255)                                                                                                                                                                              gATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTtg                                                                        <  1:740951/196‑1 (MQ=255)                                                                                                                                                                              gATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGTATAATCt                  <  1:530382/250‑1 (MQ=255)                                                                                                                                                                                         gATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCAcat                                                                                                                                     <  1:618758/124‑1 (MQ=255)                                                                                                                                                                                           tCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGTATAATCTGCGCGATATATAat    <  1:75760/251‑1 (MQ=255)                                                                                                                                                                                             tGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAg                                                                                                            <  1:398841/145‑1 (MQ=255)                                                                                                                                                                                                   gagTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGTATAATc                   <  1:98286/228‑1 (MQ=255)                                                                                                                                                                                                   gagTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGTATAATCTGCGCGATATATAat    >  1:98024/1‑243 (MQ=255)                                                                                                                                                                                                                           gAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGtat                       <  1:223610/200‑1 (MQ=255)                                                                                                                                                                                                                           gAGTAAAATTTCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGTATAATCTGCGCGATATATAat    >  1:803207/1‑219 (MQ=255)                                                                                                                                                                                                                                   tttCGGCACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGTATAATCTGCGCGATATATAattt  <  1:406896/213‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                               ACGGTAAGCGCCGTGCCGCCGCCGTTTTTGCCGCCGATATTGCGTTCCAGTAACGGTTTCGGGCTGATTTTATTCATGGCTTCAAGGTGATCCCAGGCGCTTTTGTAACTGACTTTGGCATTTTTTGCCGCTTGGTTAATGGAGCCGCATTTTTCAATTTCTTTTAATAAACGGATCCGCTTCGGATCTGCGAAGAGTTGTTGTTTCAATTTGATGGTGAGTAAAATTTCGGTACTTCGCATGTGGTTTTCCCCTCAAAATTCGCTGTGGAATATATCGTTTTATGACATGGTTTATCGGTTGCACATCATACTATATTTTGTTCTAAATAAGAACGCCGATAAATCGTTTGGCACATACGCAATCTTGTGATCTTTTCTTGTTCAAAAATATTTTTATATTGCGCTCATCGTTGTATAATCTGCGCGATATATAATTT  >  1/1518055‑1518493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑