Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,517,368	G→A	intergenic (‑72740/‑124396)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,517,368	0	G	A	100.0%	93.6 / NA	36	intergenic (‑72740/‑124396)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/20);  total (16/20)

AAAAAGGGCGGTTACGCTAACATTTATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAGAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCGGATCGACGGAAATCCAAACCTGTTGCTCAAGCTCAATATGTTGAGTTTTGGTGAGTGTAGCAC  >  1/1517147‑1517611                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    aaaaaGGGCGGTTACGCTAACATTTATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTca                                                                                                                                                                                                                        >  1:485193/1‑251 (MQ=255)       ggCGGTTACGCTAACATTTATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCacaac                                                                                                                                                                                                                    <  1:580873/249‑1 (MQ=255)           gTTACGCTAACATTTATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGaa                                                                                                                                                                                                               <  1:120464/250‑1 (MQ=255)           gTTACGCTAACATTTATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGaa                                                                                                                                                                                                               <  1:172253/250‑1 (MQ=255)               cGCTAACATTTATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATAc                                                                                                                                                                                                          <  1:864140/251‑1 (MQ=255)                 cTAACATTTATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATAcc                                                                                                                                                                                                         <  1:411296/250‑1 (MQ=255)                    aCATTTATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTgag                                                                                                                                                                                                     <  1:196803/251‑1 (MQ=255)                       tttATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTgagaga                                                                                                                                                                                                  >  1:768454/1‑251 (MQ=255)                       tttATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTgagaga                                                                                                                                                                                                  >  1:539881/1‑251 (MQ=255)                               cTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATgcgc                                                                                                                                                                                          <  1:76237/251‑1 (MQ=255)                                               aTGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATg                                                                                                                                                                           >  1:802841/1‑250 (MQ=255)                                                               ataatGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGt                                                                                                                                                          <  1:617742/251‑1 (MQ=255)                                                                     ccAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGc                                                                                                                                                     <  1:394379/250‑1 (MQ=255)                                                                         aCCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAg                                                                                                                                                <  1:224632/251‑1 (MQ=255)                                                                         aCCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAg                                                                                                                                                <  1:256100/251‑1 (MQ=255)                                                                         aCCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAg                                                                                                                                                <  1:335631/251‑1 (MQ=255)                                                                             cgATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATaaaa                                                                                                                                                                                                                                  <  1:175139/165‑1 (MQ=255)                                                                                       gTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAATAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATa                                                                                                                                  >  1:867952/1‑251 (MQ=255)                                                                                          catcaAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGCATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAAtt                                                                                                                               >  1:425343/1‑251 (MQ=255)                                                                                                    aaGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAgc                                                                                                                      <  1:687518/250‑1 (MQ=255)                                                                                                         caTTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATAcc                                                                                                                                                                                                         >  1:371291/1‑162 (MQ=255)                                                                                                                      gACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATAcc                                                                                                                                                                                                         >  1:525877/1‑149 (MQ=255)                                                                                                                               aaTGACATCAATAACGAAAATCATTCTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCGCCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAAAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAATGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACtaataa                                                                                          <  1:123510/251‑1 (MQ=255)                                                                                                                                    cATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGt                                                                                     <  1:612656/251‑1 (MQ=255)                                                                                                                                                aaaTCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAgct                                                                                                                     >  1:641222/1‑207 (MQ=255)                                                                                                                                                        cTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCg                                                                 >  1:395454/1‑251 (MQ=255)                                                                                                                                                          tttCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAg                                                                                                                                                                                                                                                 <  1:263656/73‑1 (MQ=255)                                                                                                                                                                       ttGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCGGATCGACGGAAATc                                                   >  1:571461/1‑250 (MQ=255)                                                                                                                                                                                       gAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCGGATCGACGGAAATCCAAACCTGTTgctcaag                                  >  1:557005/1‑251 (MQ=255)                                                                                                                                                                                        aGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCGGATCGACGGAAATCCAAAcc                                             >  1:894061/1‑239 (MQ=255)                                                                                                                                                                                              aaGTGGTGTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCGGATCGACGGAAATCCAAACCTGTTGCTCAAGCTCAata                           <  1:179822/251‑1 (MQ=255)                                                                                                                                                                                                  ggtgTAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCGGATCGACGGAAATCCAAACCTGTTGCTCAAGCTCAATATGtt                       >  1:141322/1‑251 (MQ=255)                                                                                                                                                                                                   gtgtAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCGGATCGACGGAAATCCAAACCTGTTGCTCAAGCTCAATATGTTg                      >  1:827406/1‑251 (MQ=255)                                                                                                                                                                                                    tgtAATCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCGGATCGACGGAAATCCAAACCTGTTGCTCAAGCTCAATATGTTg                      <  1:712782/250‑1 (MQ=255)                                                                                                                                                                                                        aTCATAAATCACCCTAAAAATAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCaa                                                                                >  1:95176/1‑188 (MQ=255)                                                                                                                                                                                                                       aaaaaTAAAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCGGATCGACGGAAATCCAAACCTGTTGCTCAAGCTCAATATGTTGAGTTTTGGTGAGTGTAGcac  <  1:520987/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    AAAAAGGGCGGTTACGCTAACATTTATGGCCTCGGTTAACCATAAAATGCCGACAAACGCCAATAATGCCAAACCGCGATTGGCTTGTTCATCAAAGGGAAGCACATTAAGAAGAATGACAAAAAGAATGACATCAATAACGAAAATCATACTTTTCTTGTTTATTTTGCTTTTCTGTGATGGAGTAAAAAGTGGTGTAATCATAAATCACCCTAAAAATAGAAAGACAAAAATCATAAAATAATATGTCACAACAGGAATATACCTGAGAGACTATGCGCTACGTCAACAAAATGCGACTTTTATGCCATGTATGGCAAAAGTGCTCCAAATGATAATTCATCGTAGCTGCTCTTCATGGAAAAAAAGACTAATAAAGGGTAGCAAGAATGATTTGCTCCGGATCGACGGAAATCCAAACCTGTTGCTCAAGCTCAATATGTTGAGTTTTGGTGAGTGTAGCAC  >  1/1517147‑1517611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑