Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,516,760	T→C	intergenic (‑72132/‑125004)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,516,760	0	T	C	100.0%	192.5 / NA	71	intergenic (‑72132/‑125004)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/44);  total (27/44)

TTTACTGAAAATCCATAGTGAAGCGACAAGGGCAAAAATCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGTGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACATAACAGCGATAAATAAACGGCCTCTTGCCAAATGCATGATTTTATTG  >  1/1516526‑1517003                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    tttACTGAAAATCCATAGTGAAGCGACAAGGGCAAAAATCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAgg                                                                                                                                                                                                                                     <  1:326845/251‑1 (MQ=255) tttACTGAAAATCCATAGTGAAGCGACAAGGGCAAAAATCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAg                                                                                                                                                                                                                                      <  1:529410/250‑1 (MQ=255)       gAAAATCCATAGTGAAGCGACAAGGGCAAAAATCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCa                                                                                                                                                                                                                               >  1:14041/1‑251 (MQ=255)           aTCCATAGTGAAGCGACAAGGGCAAAAATCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATgc                                                                                                                                                                                                                            >  1:776507/1‑250 (MQ=255)           aTCCATAGTGAAGCGACAAGGGCAAAAATCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATgc                                                                                                                                                                                                                            >  1:586115/1‑250 (MQ=255)           aTCCATAGTGAAGCGACAAGGGCAAAAATCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATgc                                                                                                                                                                                                                            >  1:658682/1‑250 (MQ=255)                               ggCAAAAATCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAg                                                                                                                                                                                                       >  1:255698/1‑251 (MQ=255)                                 cAAAAATCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCa                                                                                                                                                                                                     <  1:652652/251‑1 (MQ=255)                                     aaTCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATAc                                                                                                                                                                                                 <  1:877869/251‑1 (MQ=255)                                     aaTCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATAc                                                                                                                                                                                                 <  1:37381/251‑1 (MQ=255)                                       tCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCa                                                                                                                                                                                                                                        >  1:2344/1‑210 (MQ=255)                                              ggTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATAc                                                                                                                                                                                                 >  1:675252/1‑242 (MQ=255)                                              ggTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATa                                                                                                                                                                                        >  1:700391/1‑251 (MQ=255)                                                    ccAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCaa                                                                                                                                                                                                                                       <  1:177188/198‑1 (MQ=255)                                                           ttGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAgg                                                                                                                                                                                                                                     <  1:820153/193‑1 (MQ=255)                                                           ttGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGt                                                                                                                                                                           <  1:544448/251‑1 (MQ=255)                                                              ttATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGtat                                                                                                                                                                         <  1:204026/250‑1 (MQ=255)                                                              ttATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGtat                                                                                                                                                                         <  1:359430/250‑1 (MQ=255)                                                                  ccATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGc                                                                                                                                                                    <  1:120140/251‑1 (MQ=255)                                                                   cATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGtat                                                                                                                                                                         <  1:460402/245‑1 (MQ=255)                                                                    aTTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATAc                                                                                                                                                                                                 <  1:314346/220‑1 (MQ=255)                                                                              gTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGa                                                                                                                                                        >  1:387840/1‑251 (MQ=255)                                                                                          cATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTc                                                                                                                                            <  1:498796/251‑1 (MQ=255)                                                                                            tCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCttt                                                                                                                                                               >  1:483759/1‑230 (MQ=255)                                                                                                  cGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGAc                                                                                                                                              <  1:779786/241‑1 (MQ=255)                                                                                                       aTTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATgg                                                                                                                               >  1:363689/1‑251 (MQ=255)                                                                                                         tCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGaa                                                                                                                             <  1:448070/251‑1 (MQ=255)                                                                                                                       aaaaTAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATcatcat                                                                                                               <  1:733794/251‑1 (MQ=255)                                                                                                                        aaaTAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGa                                                                                                                                                                                              >  1:863850/1‑171 (MQ=255)                                                                                                                             atGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTAc                                                                                                                                                                                                                                  <  1:5140/130‑1 (MQ=255)                                                                                                                             atGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGt                                                                                                                                                                           <  1:874153/185‑1 (MQ=255)                                                                                                                             atGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATggcgg                                                                                                          >  1:777601/1‑250 (MQ=255)                                                                                                                                tAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGt                                                                                                      <  1:844038/251‑1 (MQ=255)                                                                                                                                         ccGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTa                                                                                               <  1:308524/249‑1 (MQ=255)                                                                                                                                            aTAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTAc                                                                                           <  1:426315/250‑1 (MQ=255)                                                                                                                                            aTAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTAc                                                                                           <  1:362533/250‑1 (MQ=255)                                                                                                                                             tAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTc                                                                                         <  1:741386/251‑1 (MQ=255)                                                                                                                                             tAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTc                                                                                         <  1:542817/251‑1 (MQ=255)                                                                                                                                             tAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTc                                                                                         <  1:276383/251‑1 (MQ=255)                                                                                                                                             tAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTc                                                                                         <  1:500209/251‑1 (MQ=255)                                                                                                                                                tGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGAc                                                                                                                                                                                 >  1:590510/1‑160 (MQ=255)                                                                                                                                                  aTTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCaa                                                                                                                                                                                                                                       <  1:898632/104‑1 (MQ=255)                                                                                                                                                      gAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACAt                                                                                 <  1:85088/250‑1 (MQ=255)                                                                                                                                                          cAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTAc                                                                                           <  1:554397/236‑1 (MQ=255)                                                                                                                                                            gTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATggc                                                                                                            >  1:94187/1‑217 (MQ=255)                                                                                                                                                             taataaCATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATaaaa                                                                         <  1:839510/251‑1 (MQ=255)                                                                                                                                                                   catcatAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCAt                                                                                                                                                                                                                              >  1:289457/1‑96 (MQ=255)                                                                                                                                                                   catcatAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTc                                                                                         <  1:386339/229‑1 (MQ=255)                                                                                                                                                                   catcatAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCgg                                                                   >  1:125154/1‑251 (MQ=255)                                                                                                                                                                        tAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATggc                                                                                                            >  1:40356/1‑205 (MQ=255)                                                                                                                                                                          ggTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCgaa                                                            >  1:816413/1‑251 (MQ=255)                                                                                                                                                                               gCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAg                                                                                                                                                                                                                                      >  1:346538/1‑76 (MQ=255)                                                                                                                                                                               gCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGa                                                                                                                                                                                  <  1:118744/128‑1 (MQ=255)                                                                                                                                                                               gCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTAc                                                                                           <  1:386037/215‑1 (MQ=255)                                                                                                                                                                                    atTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATAc                                                  <  1:546012/251‑1 (MQ=255)                                                                                                                                                                                       ttAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCTGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACATa                                               <  1:431332/251‑1 (MQ=255)                                                                                                                                                                                                   cAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACATAACAGCGataaat                                   <  1:545421/251‑1 (MQ=255)                                                                                                                                                                                                     aaaCTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACATAAc                                             >  1:570040/1‑239 (MQ=255)                                                                                                                                                                                                     aaaCTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACATAACAGCGataaataa                                 >  1:176585/1‑251 (MQ=255)                                                                                                                                                                                                             gtgtAATTGGGAGGCGGCTATAGCGTTTGGCGAACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAAGCGGCGGTGAAGAAAATATACATAACAGCGATAAATAAACAGCct                          >  1:71863/1‑250 (MQ=255)                                                                                                                                                                                                               gtAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGaa                                                                                                                             <  1:543507/149‑1 (MQ=255)                                                                                                                                                                                                               gtAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACAt                                                >  1:566744/1‑226 (MQ=255)                                                                                                                                                                                                               gtAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACATAACAGCGATAAATAAACGGCCTCtt                       >  1:832847/1‑251 (MQ=255)                                                                                                                                                                                                               gtAATTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACATAACAGCGATAAATAAACGGCCTCtt                       >  1:151012/1‑251 (MQ=255)                                                                                                                                                                                                                 aaTTGGGAGGCGGCTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACATAACAGCGATAAATAAACGGCCTCTTGc                     <  1:57098/251‑1 (MQ=255)                                                                                                                                                                                                                             gcTATAGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTa                                                                                               <  1:310609/165‑1 (MQ=255)                                                                                                                                                                                                                             gcTATAGCGTTTGGCGGACTACCTAACAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATAc                                                                                                                                                                                                 >  1:855034/1‑67 (MQ=255)                                                                                                                                                                                                                                ataGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATc                                                                                      <  1:32211/171‑1 (MQ=255)                                                                                                                                                                                                                                ataGCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATAc                                                  <  1:882084/207‑1 (MQ=255)                                                                                                                                                                                                                                   gCGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTAc                                                                                           <  1:631121/163‑1 (MQ=255)                                                                                                                                                                                                                                    cGTTTGGCGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACATAACAGCGATAAATAAACGGCCTCTTGCCAAATGCATGATTTTATTg  <  1:707134/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                    TTTACTGAAAATCCATAGTGAAGCGACAAGGGCAAAAATCACTAAGGTTAACCAGCGTTTGTTATCCATTTTCATTTGTTCAAATTGAACATCAAAACGTAAATTCAAACGTGGGCGGAAAATAATGTAAAGCCAGCCGATAATGATTGGAAACAGTAATAACATCATAGGTAAGCCATATTTTAACCAATCGGCAAAACTGAGGTGTAATTGGGAGGCGGCTATAGCGTTTGGTGGACTACCTACCAAGGTACCCATGCCGCCGATACTGGCGCTGTAAGCAATACCGAGCAATACGAAGACATAAGTATTATGCTCTTTTTGGCGATCCATTTGACTCAGAATACTCATGGAAAGCGGGATCATCATGGCGGCGGTAGCTGTATTACTCATCCACATGGATAAAAAGGCGGTGGCGAAGAAAATATACATAACAGCGATAAATAAACGGCCTCTTGCCAAATGCATGATTTTATTG  >  1/1516526‑1517003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑