Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,510,540 G→A intergenic (‑65912/‑131224) unknown ← / → unknown tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,510,5400GA100.0% 55.4 / NA 22intergenic (‑65912/‑131224)unknown/unknowntRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/12);  total (10/12)

TAGGGAGCGGTGTCCGGCGAATAAGCCTAATATACAGGAAATAAAATAGTAACTTTTGTTACTTCGTTTGGAACAACCAAACAATATGCAAACACAACATCATACTTAATTGGAAACTCTTTAACCAGTAAGAAAGTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTGTAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAATTAAACTGCTGGAAGATTTTTTAGGCGTGGAACTCTTTAAACGAAAAAACCGCGCCCTGGAACTCACGGATTTCGGCAAAGCCTATTATGCCGATATTA  >  1/1510299‑1510784
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
tAGGGAGCGGTGTCCGGCGAATAAGCCTAATATACAGGAAATAAAATAGTAACTTTTGTTACTTCGTTTGGAACAACCAAACAATATGCAAACACAACATCATACTTAATTGGAAACTCTTTAACCAGTAAGAAAGTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAAc                                                                                                                                                                                                                                             <  1:27413/251‑1 (MQ=255)
        ggTGTCCGGCGAATAAGCCTAATATACAGGAAATAAAATAGTAACTTTTGTTACTTCGTTTGGAACAACCAAACAATATGCAAACACAACATCATACTTAATTGGAAACTCTTTAACCAGTAAGAAAGTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAgg                                                                                                                                                                                                                                     >  1:791055/1‑251 (MQ=255)
                          cTAATATACAGGAAATAAAATAGTAACTTTTGTTACTTCGTTTGGAACAACCAAACAATATGCAAACACAACATCATACTTAATTGGAAACTCTTTAACCAGTAAGAAAGTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCg                                                                                                                                                                                                                   >  1:488302/1‑251 (MQ=255)
                                                       ttGTTACTTCGTTTGGAACAACCAAACAATATGCAAACACAACATCATACTTAATTGGAAACTCTTTAACCAGTAAGAAAGTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCg                                                                                                                                                                                                                   >  1:864920/1‑222 (MQ=255)
                                                                                           acacAACATCATACTTAATTGGAAACTCTTTAACCAGTAAGAAAGTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGcc                                                                                                                                                                                                             <  1:100508/192‑1 (MQ=255)
                                                                                                  atcatACTTAATTGGAAACTCTTTAACCAGTAAGAAAGTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCg                                                                                                                                                                                                                   >  1:839554/1‑179 (MQ=255)
                                                                                                                    ctctTTAACCAGTAAGAAAGTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCg                                                                                                                                                                                                                   >  1:16660/1‑161 (MQ=255)
                                                                                                                                       gTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGtat                                                                                                                                                                                                                        <  1:564114/137‑1 (MQ=255)
                                                                                                                                       gTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCaa                                                                                                      >  1:381733/1‑251 (MQ=255)
                                                                                                                                         aCCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAAt                                                                                                    >  1:145940/1‑251 (MQ=255)
                                                                                                                                                   aaGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACt                                                                                                                                                                                                  >  1:895098/1‑147 (MQ=255)
                                                                                                                                                          gttgtATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTATCCAAGCGGCGATTAGCCACCAAATTAAAc                                                                                               <  1:122810/239‑1 (MQ=255)
                                                                                                                                                             gtattgtatGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAAc                                                                                                                                                                                                   <  1:788475/136‑1 (MQ=255)
                                                                                                                                                                           aTGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAATTAAACTGCTGGAAGATTTTTTAGGCGTGGAActc                                                                  <  1:801301/251‑1 (MQ=255)
                                                                                                                                                                                             agagATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAg                                                                                                             >  1:671989/1‑190 (MQ=255)
                                                                                                                                                                                                       ttATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAATTAAACTGCTGGAAGATTTTTTAGGCGTGGAACTCTTTAAACGAAAAAACCGCGCCCTGGAAc                                      <  1:449503/251‑1 (MQ=255)
                                                                                                                                                                                                               tttATAAAAAAAAATAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAATTAAACTGCTGGAAGATTTTTTAGGCGTGGAACTCTTTAAACGAAAAAACCGCGCCCTGGAACTCACGGAt                              <  1:160088/251‑1 (MQ=255)
                                                                                                                                                                                                                     aaaaaaaaTAATCTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAATTAAACTGCTGGAAGATTTTTTAGGCGTGGAACTCTTTAAACGAAAAAACCGCGCCCTGGAACTCACGGATTTCGGc                        <  1:30783/251‑1 (MQ=255)
                                                                                                                                                                                                                                 cTGATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAATTAAACTGCTGGAAGATTTTTTAGGCGTGGAACTCTTTAAACGAAAAAACCGCGCCCTGGAACTCACGGATTTCGGCAAAGCCtattat            <  1:651919/251‑1 (MQ=255)
                                                                                                                                                                                                                                   gATTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAATTAAACTGCTGGAAGATTTTTTAGGCGTGGAACTCTTTAAACGAAAAAACCGCGCCCTGGAACTCACGGAt                              <  1:555047/231‑1 (MQ=255)
                                                                                                                                                                                                                                    aTTAACCGCATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAATTAAACTGCTGGAAGATTTTTTAGGCGTGGAACTCTTTAAACGAAAAAACCGCGCCCTGGAACTCACGGATTTCGGCAAAGCCTATTATGc          <  1:454865/250‑1 (MQ=255)
                                                                                                                                                                                                                                            cATTTATAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAATTAAACTGCTGGAAGATTTTTTAGGCGTGGAACTCTTTAAACGAAAAAACCGCGCCCTGGAACTCACGGATTTCGGCAAAGCCTATTATGCCGATATTa  >  1:251853/1‑250 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
TAGGGAGCGGTGTCCGGCGAATAAGCCTAATATACAGGAAATAAAATAGTAACTTTTGTTACTTCGTTTGGAACAACCAAACAATATGCAAACACAACATCATACTTAATTGGAAACTCTTTAACCAGTAAGAAAGTACCAAACACTAAGTATTGTTGTATTGTATGCAAAATGGAAATATTGGGCAAAAGAGATTTTTTTATTGGATTTATAAAAAAAAATAATCTGATTAACCGCATTTGTAAATAAACCATTTAGGAATTATCATGTATAAGCGTTTGCCACCGTTAAACTCGCTTAAAGCCTTTGAGGCCGCTGCCAGATTTTTAAGTTTCACCAAAGCGGCGGATGAGCTTTTTGTTACCCAAGCGGCGATTAGCCACCAAATTAAACTGCTGGAAGATTTTTTAGGCGTGGAACTCTTTAAACGAAAAAACCGCGCCCTGGAACTCACGGATTTCGGCAAAGCCTATTATGCCGATATTA  >  1/1510299‑1510784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑