Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,508,304	A→G	intergenic (‑63676/‑133460)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,508,304	0	A	G	100.0%	103.1 / NA	37	intergenic (‑63676/‑133460)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/16);  total (21/16)

GTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGAGAGTCGTGTTGATAGAAATCGTATCGATGTGCTTGTGAATATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAGTTGCCAAGAGGATAATACTTACCCAAATTA  >  1/1508063‑1508544                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 gTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAAtttt                                                                                                                                                                                                                                         >  1:146566/1‑251 (MQ=255)                   aCTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGa                                                                                                                                                                                                                       <  1:22747/251‑1 (MQ=255)                               gATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTaa                                                                                                                                                                                                                          >  1:573650/1‑236 (MQ=255)                                      gtgtCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAAt                                                                                                                                                                                                                                            <  1:59425/211‑1 (MQ=255)                                              gTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATagaag                                                                                                                                                                                                >  1:208004/1‑247 (MQ=255)                                              gTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGCCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGCAGCCAACATCACATACATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTCAACAAAAGAGTAAAGATAACAGTAATGCCAATATAGAAGATTg                                                                                                                                                                                            >  1:346634/1‑251 (MQ=255)                                                           aCGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGcc                                                                                                                                                                                                          <  1:221855/224‑1 (MQ=255)                                                            cGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTa                                                                                                                                                                              >  1:821688/1‑251 (MQ=255)                                                             gTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAatat                                                                                                                                                                                                     <  1:867881/227‑1 (MQ=255)                                                              tAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTaa                                                                                                                                                                             >  1:422394/1‑250 (MQ=255)                                                                   ggCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACtttt                                                                                                                                                                        >  1:109376/1‑250 (MQ=255)                                                                       ttGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAAc                                                                                                                                                                   <  1:76464/251‑1 (MQ=255)                                                                                                           aaaTGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAaaga                                                                                                                                <  1:457845/250‑1 (MQ=255)                                                                                                                      ggAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAAc                                                                                                                                                                            <  1:848162/195‑1 (MQ=255)                                                                                                                      ggAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTacac                                                                                                                     <  1:846402/250‑1 (MQ=255)                                                                                                                               gacgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAatat                                                                                                                                                                                                     >  1:895919/1‑161 (MQ=255)                                                                                                                                acgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                                                                                                           <  1:770713/250‑1 (MQ=255)                                                                                                                                acgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                                                                                                           <  1:805659/250‑1 (MQ=255)                                                                                                                                 cgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGAt                                                                                                                                                                                              <  1:49950/166‑1 (MQ=255)                                                                                                                                      aaCGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATg                                                                                                                                                         >  1:114795/1‑198 (MQ=255)                                                                                                                                        cGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATagaag                                                                                                                                                                                                <  1:584168/157‑1 (MQ=255)                                                                                                                                                   cAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAAcc                                                                                                                                                    >  1:465678/1‑190 (MQ=255)                                                                                                                                                     aaaTAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAg                                                                                       <  1:325748/249‑1 (MQ=255)                                                                                                                                                       aTAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAAt                                                                                   >  1:43700/1‑251 (MQ=255)                                                                                                                                                                 gTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                                                                                                           >  1:259593/1‑217 (MQ=255)                                                                                                                                                                                                       cgcgATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGAtt                                                                                              >  1:556309/1‑192 (MQ=255)                                                                                                                                                                                                         cgatagTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                                                                                                           <  1:34594/173‑1 (MQ=255)                                                                                                                                                                                                           atagTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGAc                                                                                                                            >  1:418161/3‑158 (MQ=255)                                                                                                                                                                                                           atagTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGcc                                           >  1:321986/3‑239 (MQ=255)                                                                                                                                                                                                                   gtTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGt                                                                                                                                                                                                                >  1:668745/1‑66 (MQ=255)                                                                                                                                                                                                                    ttGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAg                                <  1:510505/241‑1 (MQ=255)                                                                                                                                                                                                                      gATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGAc                                                                                                                            >  1:340229/1‑147 (MQ=255)                                                                                                                                                                                                                      gATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAGTTGCCAAGAGGa                    >  1:568391/1‑251 (MQ=255)                                                                                                                                                                                                                       aTAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAGTTGCCAAGAGGat                   <  1:52860/251‑1 (MQ=255)                                                                                                                                                                                                                           aaaTCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAAt                                                                        >  1:601038/1‑194 (MQ=255)                                                                                                                                                                                                                                      aTGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAAt                                                                        >  1:589725/1‑183 (MQ=255)                                                                                                                                                                                                                                        gtgCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAGTTGCCAAGAGGATAATACTTACCCAAATTa  >  1:2770/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 GTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGAGAGTCGTGTTGATAGAAATCGTATCGATGTGCTTGTGAATATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAGTTGCCAAGAGGATAATACTTACCCAAATTA  >  1/1508063‑1508544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑