Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,508,289	T→C	intergenic (‑63661/‑133475)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,508,289	0	T	C	100.0%	101.5 / NA	38	intergenic (‑63661/‑133475)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/18);  total (20/18)

GATAAAAACTGTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGAGAGTCGTGTTGATAGAAATCGTATCGATGTGCTTGTGAATATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAGTTGCCAAGAGGAT  >  1/1508053‑1508527                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               gATAAAAACTGTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGa                                                                                                                                                                                                                                  >  1:598189/1‑251 (MQ=255)  aTAAAAACTGTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGag                                                                                                                                                                                                                                 <  1:665274/251‑2 (MQ=255)           gTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAAtttt                                                                                                                                                                                                                        >  1:146566/1‑251 (MQ=255)                             aCTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGa                                                                                                                                                                                                      <  1:22747/251‑1 (MQ=255)                                         gATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTaa                                                                                                                                                                                                         >  1:573650/1‑236 (MQ=255)                                              aaGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTtgtg                                                                                                                                                                                                                                   <  1:621115/205‑1 (MQ=255)                                                gtgtCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAAt                                                                                                                                                                                                                           <  1:59425/211‑1 (MQ=255)                                                        gTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATagaag                                                                                                                                                                               >  1:208004/1‑247 (MQ=255)                                                        gTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGCCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGCAGCCAACATCACATACATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTCAACAAAAGAGTAAAGATAACAGTAATGCCAATATAGAAGATTg                                                                                                                                                                           >  1:346634/1‑251 (MQ=255)                                                                     aCGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGcc                                                                                                                                                                                         <  1:221855/224‑1 (MQ=255)                                                                      cGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTa                                                                                                                                                             >  1:821688/1‑251 (MQ=255)                                                                       gTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAatat                                                                                                                                                                                    <  1:867881/227‑1 (MQ=255)                                                                        tAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTaa                                                                                                                                                            >  1:422394/1‑250 (MQ=255)                                                                             ggCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACtttt                                                                                                                                                       >  1:109376/1‑250 (MQ=255)                                                                                 ttGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAAc                                                                                                                                                  <  1:76464/251‑1 (MQ=255)                                                                                                                     aaaTGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAaaga                                                                                                               <  1:457845/250‑1 (MQ=255)                                                                                                                                ggAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAAc                                                                                                                                                           <  1:848162/195‑1 (MQ=255)                                                                                                                                ggAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTacac                                                                                                    <  1:846402/250‑1 (MQ=255)                                                                                                                                         gacgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAatat                                                                                                                                                                                    >  1:895919/1‑161 (MQ=255)                                                                                                                                          acgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                                                                                          <  1:805659/250‑1 (MQ=255)                                                                                                                                          acgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                                                                                          <  1:770713/250‑1 (MQ=255)                                                                                                                                           cgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGAt                                                                                                                                                                             <  1:49950/166‑1 (MQ=255)                                                                                                                                                aaCGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATg                                                                                                                                        >  1:114795/1‑198 (MQ=255)                                                                                                                                                  cGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATagaag                                                                                                                                                                               <  1:584168/157‑1 (MQ=255)                                                                                                                                                             cAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAAcc                                                                                                                                   >  1:465678/1‑190 (MQ=255)                                                                                                                                                               aaaTAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAg                                                                      <  1:325748/249‑1 (MQ=255)                                                                                                                                                                 aTAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAAt                                                                  >  1:43700/1‑251 (MQ=255)                                                                                                                                                                           gTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                                                                                          >  1:259593/1‑217 (MQ=255)                                                                                                                                                                                                                 cgcgATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGAtt                                                                             >  1:556309/1‑192 (MQ=255)                                                                                                                                                                                                                   cgatagTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                                                                                          <  1:34594/173‑1 (MQ=255)                                                                                                                                                                                                                     atagTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGAc                                                                                                           >  1:418161/3‑158 (MQ=255)                                                                                                                                                                                                                     atagTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGcc                          >  1:321986/3‑239 (MQ=255)                                                                                                                                                                                                                             gtTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGt                                                                                                                                                                                               >  1:668745/1‑66 (MQ=255)                                                                                                                                                                                                                              ttGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAg               <  1:510505/241‑1 (MQ=255)                                                                                                                                                                                                                                gATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGAc                                                                                                           >  1:340229/1‑147 (MQ=255)                                                                                                                                                                                                                                gATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAGTTGCCAAGAGGa   >  1:568391/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAGTTGCCAAGAGGat  <  1:52860/251‑1 (MQ=255)                                                                                                                                                                                                                                     aaaTCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAAt                                                       >  1:601038/1‑194 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                               GATAAAAACTGTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGAGAGTCGTGTTGATAGAAATCGTATCGATGTGCTTGTGAATATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAATCGGTCATGAATGGTCTGGCTTTTTTTAACCCAAACAAGCCAACTAAATAAGTTGCCAAGAGGAT  >  1/1508053‑1508527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑