Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,508,266	G→T	intergenic (‑63638/‑133498)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,508,266	0	G	T	100.0%	99.1 / NA	35	intergenic (‑63638/‑133498)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/19);  total (16/19)

ATAAAAAATTAATATTCATTTTATAAAGATAAAAACTGTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGAGAGTCGTGTTGATAGAAATCGTATCGATGTGCTTGTGAATATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAAT  >  1/1508026‑1508463                                                                                                                                                                                                                                                 |                                                                                                                                                                                                      aTAAAAAATTAATATTCATTTTATAAAGATAAAAACTGTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTg                                                                                                                                                                                             <  1:849709/251‑1 (MQ=255)               ttCATTTTATAAAGATAAAAACTGTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTacc                                                                                                                                                                               <  1:279100/251‑3 (MQ=255)                            gATAAAAACTGTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGa                                                                                                                                                                  >  1:598189/1‑251 (MQ=255)                             aTAAAAACTGTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGag                                                                                                                                                                 <  1:665274/251‑2 (MQ=255)                                      gTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAAtttt                                                                                                                                                        >  1:146566/1‑251 (MQ=255)                                              tagCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAAt                                                                                                                                                                                     >  1:304856/1‑214 (MQ=255)                                                 cACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAAt                                                                                                                                                                                     <  1:762582/211‑1 (MQ=255)                                                        aCTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGa                                                                                                                                      <  1:22747/251‑1 (MQ=255)                                                                   aGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGt                                                                                                                                                                                                    <  1:345495/178‑1 (MQ=255)                                                                    gATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTaa                                                                                                                                         >  1:573650/1‑236 (MQ=255)                                                                         aaGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTtgtg                                                                                                                                                                   <  1:621115/205‑1 (MQ=255)                                                                           gtgtCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAAt                                                                                                                                                           <  1:59425/211‑1 (MQ=255)                                                                                   gTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATagaag                                                                                                               >  1:208004/1‑247 (MQ=255)                                                                                   gTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGCCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGCAGCCAACATCACATACATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTCAACAAAAGAGTAAAGATAACAGTAATGCCAATATAGAAGATTg                                                                                                           >  1:346634/1‑251 (MQ=255)                                                                                                aCGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGcc                                                                                                                         <  1:221855/224‑1 (MQ=255)                                                                                                 cGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTa                                                                                             >  1:821688/1‑251 (MQ=255)                                                                                                  gTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAatat                                                                                                                    <  1:867881/227‑1 (MQ=255)                                                                                                   tAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTaa                                                                                            >  1:422394/1‑250 (MQ=255)                                                                                                        ggCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACtttt                                                                                       >  1:109376/1‑250 (MQ=255)                                                                                                            ttGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAAc                                                                                  <  1:76464/251‑1 (MQ=255)                                                                                                                                                aaaTGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAaaga                                               <  1:457845/250‑1 (MQ=255)                                                                                                                                                           ggAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAAc                                                                                           <  1:848162/195‑1 (MQ=255)                                                                                                                                                           ggAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTacac                                    <  1:846402/250‑1 (MQ=255)                                                                                                                                                                    gacgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAatat                                                                                                                    >  1:895919/1‑161 (MQ=255)                                                                                                                                                                     acgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                          <  1:805659/250‑1 (MQ=255)                                                                                                                                                                     acgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                          <  1:770713/250‑1 (MQ=255)                                                                                                                                                                      cgaTAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGAt                                                                                                             <  1:49950/166‑1 (MQ=255)                                                                                                                                                                           aaCGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATg                                                                        >  1:114795/1‑198 (MQ=255)                                                                                                                                                                             cGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATagaag                                                                                                               <  1:584168/157‑1 (MQ=255)                                                                                                                                                                                   tatTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTg                                                                                                                                                                                             >  1:662112/1‑73 (MQ=255)                                                                                                                                                                                        cAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAAcc                                                                   >  1:465678/1‑190 (MQ=255)                                                                                                                                                                                          aaaTAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAg      <  1:325748/249‑1 (MQ=255)                                                                                                                                                                                            aTAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAAt  >  1:43700/1‑251 (MQ=255)                                                                                                                                                                                                      gTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAg                          >  1:259593/1‑217 (MQ=255)                                                                                                                                                                                                                                            cgcgATAGTCGTGTTGATAGAAATCGTACCGATGTGCTTGTGAGTATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGAtt             >  1:556309/1‑192 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                      ATAAAAAATTAATATTCATTTTATAAAGATAAAAACTGTCATTAGTAGCACGAGTACTTTTCGCTAAGATAAAAGTGTCACTGTATTTTGCTAATACGTAATAGGCTTTGCCTTCAATTTCTAATGAGTCAAATACTGTTTTGAAATGTGGGCGGGAATATCCGACGATAAACGCACATATTACAAAATAACAATAGGTAGCCAACATCACATAAATTTTATTTTTATATAAAAACGCGAGAGTCGTGTTGATAGAAATCGTATCGATGTGCTTGTGAATATAATTTTTAAACAAAAGAGTAAAGATAAAAGTAATGCCAATATAGAAGATTGCGAAAAGATAATTAACTTTTCCAACTGTCAGGATGTAACCAAGAATAACCGGAATAAAGAAGACATTACACAGAATATAAGTCCGCAACAGATTCATGCAGTAAT  >  1/1508026‑1508463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑