Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,506,710	G→A	intergenic (‑62082/‑135054)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,506,710	0	G	A	100.0%	58.5 / NA	23	intergenic (‑62082/‑135054)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/8);  total (15/8)

GGTTTACGCTACGAAAACCAAGTTTTTGAAGCTCGGCGGCAATTTCCGTTTGTGAACCGTATTTTTCTTCGCGTAATAAAGTTTTAAATGCTTTGACCAGATTGTCCGGATTTTTATTCAACATAGGTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACGGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGAATGAAATGAAAGTTGCTGTTTTAGGTGCCGCAGGCGGTATCGGTCAAGCATTGGCATTATTACTGAAGTTACAA  >  1/1506506‑1506953                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    ggTTTACGCTACGAAAACCAAGTTTTTGAAGCTCGGCGGCAATTTCCGTTTGTGAACCGTATTTTTCTTCGCATAATAAAGTTTTAAATGCTTTGACCAGATTGTCCGGATTTTTATTCAACATAGGTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGtt                                                                                                                                                                                                       >  1:53703/1‑251 (MQ=255)          tACGAAAACCAAGTTTTTGAAGCTCGGCGGCAATTTCCGTTTGTGAACCGTATTTTTCTTCGCATAATAAAGTTTTAAATGCTTTGACCAGATTGTCCGGATTTTTATTCAACATAGGTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGaaa                                                                                                                                                                                              <  1:865667/251‑1 (MQ=255)                                                          cGTATTTTTCTTCGCATAATAAAGTTTTAAATGCTTTGACCAGATTGTCCGGATTTTTATTCAACATAGGTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAAtt                                                                                                                                              <  1:257806/251‑1 (MQ=255)                                                                                 gTTTTAAATGCTTTGACCAGATTGTCCGGATTTTTATTCAACATAGGTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAAtt                                                                                                                       >  1:649676/1‑251 (MQ=255)                                                                                                               tttttATTCAACATAGGTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTta                                                                                         >  1:730544/1‑251 (MQ=255)                                                                                                                ttttATTCAACATAGGTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTtac                                                                                        <  1:163267/251‑1 (MQ=255)                                                                                                                       cAACATAGGTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATga                                                                                 >  1:152301/1‑251 (MQ=255)                                                                                                                         aCATAGGTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAACAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATgagg                                                                               >  1:164674/1‑251 (MQ=255)                                                                                                                              ggTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGaat                                                                          >  1:562895/1‑251 (MQ=255)                                                                                                                                   gTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTAc                                                                                                                           >  1:471178/1‑197 (MQ=255)                                                                                                                                              aaCATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGAATGAAATGAAAGTTGCTg                                                          <  1:742296/251‑1 (MQ=255)                                                                                                                                               aCATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGaaa                                                                                                                                                                                                                                                 >  1:766560/1‑67 (MQ=255)                                                                                                                                                 aTAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGAATGAAATGAAAGTTGCTGttt                                                       <  1:288355/251‑1 (MQ=255)                                                                                                                                                     gTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGAATGAAATGAAAGTTGCTGTTTTAgg                                                   >  1:249521/1‑251 (MQ=255)                                                                                                                                                                    aTAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGAATGAAATGAAAGTTGCTGTTTTAGGTGCCGCAGGCGGTAt                                    <  1:774904/251‑1 (MQ=255)                                                                                                                                                                          gCAGTGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGAATGAAATGAAAGTTGCTGTTTTAGGt                                                  >  1:897674/1‑231 (MQ=255)                                                                                                                                                                              tGACTTTAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGAATGAAATGAAAGTTGCTGTTTTAGGTGCCGCAGGCGGTATCGGTCAAGCa                          <  1:268940/251‑1 (MQ=255)                                                                                                                                                                                    tAGCAAATAAATATTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAac                                                                                                      >  1:850940/1‑169 (MQ=255)                                                                                                                                                                                        gaataaataTTTAATTAAAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCAc                                                                                                                                                             >  1:797215/2‑110 (MQ=255)                                                                                                                                                                                                    aattaaAACAGAAAAGTGCGGTCATAAAAAATTATGTTTTTtg                                                                                                                                                                                                                    >  1:676789/1‑43 (MQ=255)                                                                                                                                                                                                         aaaCAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTtac                                                                                        >  1:893633/1‑162 (MQ=255)                                                                                                                                                                                                         aaaCAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGAATGAAATGAAAGTTGCTGTTTTAGGTGCCGCAGGCGGTAt                                    <  1:659306/214‑1 (MQ=255)                                                                                                                                                                                                         aaaCAGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGAATGAAATGAAAGTTGCTGTTTTAGGTGCCGCAGGCGGTATCGGTCAAGCATTGGCATTATTACTGAAGTTACaa  >  1:172700/1‑248 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    GGTTTACGCTACGAAAACCAAGTTTTTGAAGCTCGGCGGCAATTTCCGTTTGTGAACCGTATTTTTCTTCGCGTAATAAAGTTTTAAATGCTTTGACCAGATTGTCCGGATTTTTATTCAACATAGGTTTGTCTCTTGTGGAACATAAGTGGCTGAATTTTGCATAAAAGCAGTGACTTTAGCAAATAAATATTTAATTAAAACGGAAAAGTGCGGTCATAAAAAATTATGTTTTTTGTGATTTTTATGTTCAATAGAAAATAAAAAATTTGAGTTAGATCGCACATTTGCACCTGCCTGTTTGAATTTAAAAATTGATCAGCGTACAATTTTTTCAGATTTTTTAACCACCTATTTTTTACTACATGAGGAGAATGAAATGAAAGTTGCTGTTTTAGGTGCCGCAGGCGGTATCGGTCAAGCATTGGCATTATTACTGAAGTTACAA  >  1/1506506‑1506953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑