Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,485,748	T→C	intergenic (‑41120/‑156016)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,485,748	0	T	C	100.0%	67.2 / NA	25	intergenic (‑41120/‑156016)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/14);  total (11/14)

CACAACCGGGATAGACTTTAATGACCGTGCCGATTGAAAGCTTTTGCGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACCGCGCGCACCTCGCGCACGCCGTCATCCTGCCACTCGATAAAACCCGCATTAAACCAGCCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCGCTGATTGTAAGCCCCGCCACCACAAAGTTGCCGGGCTTGACTTTGCAATCGGTATCGTAAAGCGTATATGGGGAAGGAACGTCTTGCTT  >  1/1485504‑1485795                                                                                                                                                                                                                                                     |                                                cacagcccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCaa                                           <  1:556426/243‑1 (MQ=255)     gcccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCgg                                       >  1:716353/5‑251 (MQ=255)               gcTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCACCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:600062/248‑1 (MQ=255)               aCTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:902005/249‑1 (MQ=255)               aCTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:729176/249‑1 (MQ=255)               aCTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:649664/249‑1 (MQ=255)                 tttAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCg                           <  1:856852/251‑1 (MQ=255)                  ttAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGt                          >  1:127807/1‑251 (MQ=255)                  ttAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGt                          >  1:879014/1‑251 (MQ=255)                   taattacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAg                             <  1:405102/239‑1 (MQ=255)                    aattacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtat                        >  1:671708/8‑251 (MQ=255)                      ttacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatac                      <  1:518966/246‑2 (MQ=255)                       tacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacg                     <  1:875676/247‑3 (MQ=255)                                        gCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGTATACgggctgtgacggacggg     >  1:598881/1‑236 (MQ=255)                                         cTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattacgccc        <  1:246871/246‑16 (MQ=255)                                           tttGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCaa                                           >  1:26111/1‑209 (MQ=255)                                           tttGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattacgcccccaggt  >  1:134146/1‑229 (MQ=255)                                              gtgTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAg                             >  1:20524/3‑220 (MQ=255)                                                      cccATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:755293/210‑1 (MQ=255)                                                         aTTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCg                           <  1:403550/211‑1 (MQ=255)                                                                                       gTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattacgc          >  1:886884/1‑185 (MQ=255)                                                                                            tgcccgcACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaatta             >  1:66382/5‑180 (MQ=255)                                                                                                                               caaTAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               >  1:271276/3‑137 (MQ=255)                                                                                                                                 aTAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:421825/135‑1 (MQ=255)                                                                                                                                               aaaCCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattac            <  1:819804/140‑12 (MQ=255)                                                                                                                                                                                                                                                     |                                                CACAACCGGGATAGACTTTAATGACCGTGCCGATTGAAAGCTTTTGCGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACCGCGCGCACCTCGCGCACGCCGTCATCCTGCCACTCGATAAAACCCGCATTAAACCAGCCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCGCTGATTGTAAGCCCCGCCACCACAAAGTTGCCGGGCTTGACTTTGCAATCGGTATCGTAAAGCGTATATGGGGAAGGAACGTCTTGCTT  >  1/1485504‑1485795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑