Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,485,709	G→A	intergenic (‑41081/‑156055)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,485,709	0	G	A	100.0%	113.7 / NA	42	intergenic (‑41081/‑156055)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/23);  total (19/23)

CATATTGTTAAATTTTTTAAGACAGGTTTGTGCCCGTCCGTCACAACCGGGATAGACTTTAATGACCGTGCCGATTGAAAGCTTTTGCGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACCGCGCGCACCTCGCGCACGCCGTCATCCTGCCACTCGATAAAACCCGCATTAAACCAGCCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCGCTGATTGTAAGCCCCGCCACCACAAAGTTGCCGGGCTTGACTTTGCAATCGGTATCGTAAAGCGTATATGGGGAAGGAACGTCTTGCTT  >  1/1485463‑1485795                                                                                                                                                                                                                                                       |                                                                                       cATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTg                                                                                    <  1:160908/251‑1 (MQ=255)      tGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGc                                                                               <  1:39332/251‑1 (MQ=255)      tGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGc                                                                               <  1:871529/251‑1 (MQ=255)       gTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGc                                                                               >  1:533816/1‑250 (MQ=255)                          gTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGc                                                            >  1:516917/1‑250 (MQ=255)                              gcgCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCggg                                                        >  1:353701/3‑250 (MQ=255)                                  gggggcgtaattgtccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGc                                                                               >  1:739306/17‑223 (MQ=255)                                   cgtccgtcACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCttaac                                                  <  1:47607/251‑4 (MQ=255)                                          cacagcccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCaa                                           <  1:556426/243‑1 (MQ=255)                                              gcccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTg                                                                                    >  1:488406/5‑206 (MQ=255)                                              gcccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGcc                                                                              <  1:848197/208‑1 (MQ=255)                                              gcccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCgg                                       >  1:716353/5‑251 (MQ=255)                                                  ggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTa                                                                                  <  1:166489/204‑1 (MQ=255)                                                        gcTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCACCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:600062/248‑1 (MQ=255)                                                        aCTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:729176/249‑1 (MQ=255)                                                        aCTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:649664/249‑1 (MQ=255)                                                        aCTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:902005/249‑1 (MQ=255)                                                         cTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAg                                                                                >  1:406400/1‑199 (MQ=255)                                                          tttAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCg                           <  1:856852/251‑1 (MQ=255)                                                           ttAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGt                          >  1:127807/1‑251 (MQ=255)                                                           ttAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGt                          >  1:879014/1‑251 (MQ=255)                                                            taattacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAg                             <  1:405102/239‑1 (MQ=255)                                                             aattacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtat                        >  1:671708/8‑251 (MQ=255)                                                               ttacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatac                      <  1:518966/246‑2 (MQ=255)                                                                tacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacg                     <  1:875676/247‑3 (MQ=255)                                                                                 gCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCttaacc                                                 >  1:393205/1‑202 (MQ=255)                                                                                 gCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGTATACgggctgtgacggacggg     >  1:598881/1‑236 (MQ=255)                                                                                  cTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattacgccc        <  1:246871/246‑16 (MQ=255)                                                                                    tttGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCaa                                           >  1:26111/1‑209 (MQ=255)                                                                                    tttGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattacgcccccaggt  >  1:134146/1‑229 (MQ=255)                                                                                       gtgTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAg                             >  1:20524/3‑220 (MQ=255)                                                                                               cccATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:755293/210‑1 (MQ=255)                                                                                                 cATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTg                                                                                    <  1:496350/155‑1 (MQ=255)                                                                                                 cATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTg                                                                                    >  1:726503/1‑155 (MQ=255)                                                                                                  aTTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCg                           <  1:403550/211‑1 (MQ=255)                                                                                                                                gTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCttaacc                                                 <  1:108953/159‑5 (MQ=255)                                                                                                                                gTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattacgc          >  1:886884/1‑185 (MQ=255)                                                                                                                                     tgcccgcACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaatta             >  1:66382/5‑180 (MQ=255)                                                                                                                                                                        caaTAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGcc                                                                              <  1:323747/88‑1 (MQ=255)                                                                                                                                                                        caaTAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               >  1:271276/3‑137 (MQ=255)                                                                                                                                                                          aTAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:421825/135‑1 (MQ=255)                                                                                                                                                                                        aaaCCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattac            <  1:819804/140‑12 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                       CATATTGTTAAATTTTTTAAGACAGGTTTGTGCCCGTCCGTCACAACCGGGATAGACTTTAATGACCGTGCCGATTGAAAGCTTTTGCGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACCGCGCGCACCTCGCGCACGCCGTCATCCTGCCACTCGATAAAACCCGCATTAAACCAGCCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCGCTGATTGTAAGCCCCGCCACCACAAAGTTGCCGGGCTTGACTTTGCAATCGGTATCGTAAAGCGTATATGGGGAAGGAACGTCTTGCTT  >  1/1485463‑1485795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑