Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,485,631	G→A	intergenic (‑41003/‑156133)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,485,631	0	G	A	100.0%	207.5 / NA	76	intergenic (‑41003/‑156133)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/40);  total (36/40)

AATCAAATCCCTCTAAAACACGCGTGAACCGTCATACGGCGATTTGGTCGGCATATGTGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGTGCCCGTCCGTCACAACCGGGATAGACTTTAATGACCGTGCCGATTGAAAGCTTTTGCGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACCGCGCGCACCTCGCGCACGCCGTCATCCTGCCACTCGATAAAACCCGCATTAAACCAGCCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCGCTGATTGTAAGCCCCGCCACCACAAAGTTGCCGGGCTTGACTTTGCAATCGGTATCGTAAAGCGTATATGGGGAAGGAACGTCTTGCTT  >  1/1485385‑1485795                                                                                                                                                                                                                                                       |                                                                                                                                                                     taataaatcctcctAAAACACGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATaa                                                                                                                                                                  >  1:815458/13‑251 (MQ=255)    taaatcctcctAAAACACGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAAc                                                                                                                                                               <  1:152032/242‑1 (MQ=255)    taaatcctcctAAAACACGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAAc                                                                                                                                                               <  1:460704/242‑1 (MQ=255)     aaatcctcctAAAACACGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAAcc                                                                                                                                                              <  1:782621/243‑1 (MQ=255)     aaatcctcctAAAACACGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAAcc                                                                                                                                                              <  1:135486/243‑1 (MQ=255)     aaatcctcctAAAACACGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAAcc                                                                                                                                                              <  1:203149/243‑1 (MQ=255)     aaatcctcctAAAACACGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAAcc                                                                                                                                                              <  1:73825/243‑1 (MQ=255)      aatcctcctAAAACACGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAAcct                                                                                                                                                             <  1:726845/244‑2 (MQ=255)            cctAAAACACGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTa                                                                                                                                                       >  1:724921/2‑251 (MQ=255)                   cacGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCaac                                                                                                                                                <  1:819735/251‑3 (MQ=255)                    acGCGTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCaacc                                                                                                                                               <  1:223269/251‑4 (MQ=255)                     cgcgTGAACCGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAAcc                                                                                                                                                              >  1:85107/1‑235 (MQ=255)                             ccGTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAg                                                                                                                                      >  1:217047/1‑251 (MQ=255)                               gTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGt                                                                                                                                    >  1:480816/1‑251 (MQ=255)                               gTCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGt                                                                                                                                    >  1:375749/1‑251 (MQ=255)                                tCATACGGCGATTTGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCaacc                                                                                                                                               >  1:532475/1‑236 (MQ=255)                                             tGGTTGGCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGAttt                                                                                                                      >  1:81734/1‑251 (MQ=255)                                                 tggCATGTGCGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACgg                                                                                                                  <  1:874022/250‑1 (MQ=255)                                                    catgtgcggAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGa                                                                                                               <  1:678237/244‑1 (MQ=255)                                                       gtgcggAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAAc                                                                                                                                                    <  1:700383/207‑1 (MQ=255)                                                       gtgcggAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATgg                                                                                                            >  1:775486/5‑251 (MQ=255)                                                       gtgcggAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATgg                                                                                                            >  1:785450/5‑251 (MQ=255)                                                       gtgcggAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATgg                                                                                                            >  1:512153/5‑251 (MQ=255)                                                       gtgcggAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATgg                                                                                                            >  1:89418/5‑251 (MQ=255)                                                         gcggAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGa                                                                                                           >  1:206296/3‑250 (MQ=255)                                                            gAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGCGATGGACGc                                                                                                        >  1:304945/1‑250 (MQ=255)                                                                 ccgccgAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTc                                                                                                  <  1:713664/251‑1 (MQ=255)                                                                  cgccgAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTcc                                                                                                 <  1:27817/251‑1 (MQ=255)                                                                         aTTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACgg                                                                                                                  <  1:562887/227‑1 (MQ=255)                                                                         aTTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATcgcac                                                                                          <  1:894604/251‑3 (MQ=255)                                                                           tAAGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGG‑TTGCCGGCAGATTTACGGTGATGGACGCCCCGTc                                                                                                  <  1:312941/240‑1 (MQ=255)                                                                            aaGCATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAAcc                                                                                                                                                              >  1:189578/1‑180 (MQ=255)                                                                               cATATTGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTg                                                                                    <  1:160908/251‑1 (MQ=255)                                                                                   ttGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGAc                                                                                                          >  1:680932/1‑225 (MQ=255)                                                                                    tGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGc                                                                               <  1:39332/251‑1 (MQ=255)                                                                                    tGTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGc                                                                               <  1:871529/251‑1 (MQ=255)                                                                                     gTTAAATTTTTTAAGACAGGTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGc                                                                               >  1:533816/1‑250 (MQ=255)                                                                                                       ggTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACgg                                                                                                                  <  1:497276/197‑1 (MQ=255)                                                                                                       ggTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACgg                                                                                                                  >  1:585491/1‑197 (MQ=255)                                                                                                        gTTTGCGCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGc                                                            >  1:516917/1‑250 (MQ=255)                                                                                                            gcgCCCGTCCGTCACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCggg                                                        >  1:353701/3‑250 (MQ=255)                                                                                                                gggggcgtaattgtccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGc                                                                               >  1:739306/17‑223 (MQ=255)                                                                                                                 cgtccgtcACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCttaac                                                  <  1:47607/251‑4 (MQ=255)                                                                                                                      gtcACAGCCCGGATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGccc                                                                                                      <  1:457492/194‑1 (MQ=255)                                                                                                                        cacagcccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCaa                                           <  1:556426/243‑1 (MQ=255)                                                                                                                            gcccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTg                                                                                    >  1:488406/5‑206 (MQ=255)                                                                                                                            gcccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGcc                                                                              <  1:848197/208‑1 (MQ=255)                                                                                                                            gcccggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCgg                                       >  1:716353/5‑251 (MQ=255)                                                                                                                                ggATAGACTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTa                                                                                  <  1:166489/204‑1 (MQ=255)                                                                                                                                      gcTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCACCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:600062/248‑1 (MQ=255)                                                                                                                                      aCTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:729176/249‑1 (MQ=255)                                                                                                                                      aCTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:902005/249‑1 (MQ=255)                                                                                                                                      aCTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:649664/249‑1 (MQ=255)                                                                                                                                       cTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCaacc                                                                                                                                               >  1:292106/1‑133 (MQ=255)                                                                                                                                       cTTTAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAg                                                                                >  1:406400/1‑199 (MQ=255)                                                                                                                                        tttAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCg                           <  1:856852/251‑1 (MQ=255)                                                                                                                                         ttAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGt                          >  1:127807/1‑251 (MQ=255)                                                                                                                                         ttAATTACGGTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGt                          >  1:879014/1‑251 (MQ=255)                                                                                                                                          taattacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAg                             <  1:405102/239‑1 (MQ=255)                                                                                                                                           aattacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtat                        >  1:671708/8‑251 (MQ=255)                                                                                                                                             ttacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatac                      <  1:518966/246‑2 (MQ=255)                                                                                                                                              tacggTGCCGATTGCAAGCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacg                     <  1:875676/247‑3 (MQ=255)                                                                                                                                                               gCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCttaacc                                                 >  1:393205/1‑202 (MQ=255)                                                                                                                                                               gCTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGTATACgggctgtgacggacggg     >  1:598881/1‑236 (MQ=255)                                                                                                                                                                cTTTTGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattacgccc        <  1:246871/246‑16 (MQ=255)                                                                                                                                                                  tttGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCaa                                           >  1:26111/1‑209 (MQ=255)                                                                                                                                                                  tttGTGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattacgcccccaggt  >  1:134146/1‑229 (MQ=255)                                                                                                                                                                     gtgTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAg                             >  1:20524/3‑220 (MQ=255)                                                                                                                                                                             cccATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGAc                                                                                                          <  1:821724/135‑1 (MQ=255)                                                                                                                                                                             cccATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATaa                               <  1:755293/210‑1 (MQ=255)                                                                                                                                                                               cATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTg                                                                                    >  1:726503/1‑155 (MQ=255)                                                                                                                                                                               cATTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTg                                                                                    <  1:496350/155‑1 (MQ=255)                                                                                                                                                                                aTTAATGTCAGCTTGTTATTTTGATGCACGGTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCg                           <  1:403550/211‑1 (MQ=255)                                                                                                                                                                                                              gTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCttaacc                                                 <  1:108953/159‑5 (MQ=255)                                                                                                                                                                                                              gTGACTGCCCGCACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaattacgc          >  1:886884/1‑185 (MQ=255)                                                                                                                                                                                                                   tgcccgcACCTCGCGCACGCCGTCATCCTGCCACTCAATAAAACCTGCATTAAACCAACCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCACTAATTGTAAGCCCCGCCACCACAAAATTGCCGGGCTTAACCTTGCAATCGGTATCATAAAGCGtatacggacaatta             >  1:66382/5‑180 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                     AATCAAATCCCTCTAAAACACGCGTGAACCGTCATACGGCGATTTGGTCGGCATATGTGGAATACCGCCGAAATTAAGCATATTGTTAAATTTTTTAAGACAGGTTTGTGCCCGTCCGTCACAACCGGGATAGACTTTAATGACCGTGCCGATTGAAAGCTTTTGCGTGCCACCCATTAATGTCAGCTTGTTATTTTGATGCACGGTGACCGCGCGCACCTCGCGCACGCCGTCATCCTGCCACTCGATAAAACCCGCATTAAACCAGCCTTGCGGCAGGTTTGCCGGCAGATTTACGGTGATGGACGCCCCGTCCATCGCGCTGATTGTAAGCCCCGCCACCACAAAGTTGCCGGGCTTGACTTTGCAATCGGTATCGTAAAGCGTATATGGGGAAGGAACGTCTTGCTT  >  1/1485385‑1485795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑