Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,498	C→G	intergenic (‑39870/‑157266)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,498	0	C	G	100.0%	180.7 / NA	66	intergenic (‑39870/‑157266)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (36/30);  total (36/30)

GTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGTTTGATTATTTGCCGCCGACGGGTTATACACACCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGCTCACCCATCAGGATTTCCATTCGCCCCTGGATGCCTCCCTCACCTCCGGTGTTATCACC  >  1/1484308‑1484737                                                                                                                                                                                               |                                                                                                                                                                                                                                                gctctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                                                                                  >  1:70762/18‑250 (MQ=255)    ctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTAtgc                                                                                                                                                                               <  1:436180/237‑1 (MQ=255)      gccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAttatg                                                                                                                                                                                >  1:681245/13‑245 (MQ=255)                            gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTg                                                                                                                                                       >  1:865665/1‑251 (MQ=255)                            gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGCAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAgcgcctt                                                                                                                                                       >  1:834943/1‑248 (MQ=255)                                gTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTgcgc                                                                                                                                                    >  1:30435/1‑250 (MQ=255)                                 tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCtttttt                                                                                                                                                                 >  1:515774/1‑236 (MQ=255)                                 tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAg                                                                                                                                                             <  1:841382/240‑1 (MQ=255)                                      gcgcAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                                                                                  >  1:826628/1‑213 (MQ=255)                                                      catttttCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTgc                                                                                                                             <  1:149503/249‑1 (MQ=255)                                                                    gtaccataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGTGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGt                                                                                                                  >  1:772783/11‑251 (MQ=255)                                                                    gtaccataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGatta                                                                                                                                  >  1:845900/11‑235 (MQ=255)                                                                      accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGatta                                                                                                                                  <  1:508408/225‑1 (MQ=255)                                                                      accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTta                                                                                                                >  1:826927/9‑251 (MQ=255)                                                                      accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTta                                                                                                                >  1:430838/9‑251 (MQ=255)                                                                         ataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACGCAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATac                                                                                                             <  1:612641/246‑1 (MQ=255)                                                                         ataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGt                                                                                                                  <  1:70541/241‑1 (MQ=255)                                                                              ccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATacacccc                                                                                                        <  1:819858/251‑4 (MQ=255)                                                                              ccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATacac                                                                                                           <  1:759106/248‑1 (MQ=255)                                                                                   gtgCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCggct                                                                                                                                        <  1:511702/214‑3 (MQ=255)                                                                                      ccAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                                                                 <  1:22678/250‑1 (MQ=255)                                                                                      ccAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                                                                 <  1:51602/250‑1 (MQ=255)                                                                                          ccTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                                                                 <  1:384705/246‑1 (MQ=255)                                                                                            tttGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                                                                                  >  1:312476/1‑162 (MQ=255)                                                                                            tttGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTaa                                                                                          >  1:106708/1‑251 (MQ=255)                                                                                                        gcgcagacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                                                                 <  1:891950/225‑1 (MQ=255)                                                                                                        gcgcagacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTggg                                                                              >  1:346796/8‑251 (MQ=255)                                                                                                             gacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAACCACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATac                                                                                                             <  1:892568/213‑1 (MQ=255)                                                                                                               cGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtt                                                                       >  1:431427/1‑251 (MQ=255)                                                                                                                 gTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtttt                                                                     >  1:328909/1‑251 (MQ=255)                                                                                                                 gTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtttt                                                                     >  1:555558/1‑251 (MQ=255)                                                                                                                     cTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTc                                                                 <  1:513218/251‑1 (MQ=255)                                                                                                                       gtgttttttAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCg                                                               >  1:61062/5‑251 (MQ=255)                                                                                                                         gttgtttAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTGCACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATTCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTgccgc                                                                                                                          <  1:770145/186‑1 (MQ=255)                                                                                                                                       gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAg                                                                                                  >  1:394380/1‑200 (MQ=255)                                                                                                                                       gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTc                                                                 >  1:678302/1‑233 (MQ=255)                                                                                                                                       gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaat                                               >  1:554196/1‑245 (MQ=255)                                                                                                                                       gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaat                                               >  1:372824/1‑245 (MQ=255)                                                                                                                                                caacTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttgaaatctccatac                                       <  1:470632/247‑15 (MQ=255)                                                                                                                                                  acTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaatctccatacgac                                    <  1:600546/250‑18 (MQ=255)                                                                                                                                                   cTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaatctccatacgac                                    >  1:56549/1‑233 (MQ=255)                                                                                                                                                          ggCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATc                                                                                        <  1:577690/191‑1 (MQ=255)                                                                                                                                                           gcctgAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACGCTGCCGGGGCTGACATTGCAGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGAt                             <  1:341952/245‑1 (MQ=255)                                                                                                                                                           gcctgAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGAt                             <  1:872816/245‑1 (MQ=255)                                                                                                                                                           gcctgAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGAt                             <  1:28247/245‑1 (MQ=255)                                                                                                                                                           gcctgAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGAt                             <  1:580596/245‑1 (MQ=255)                                                                                                                                                             ctgAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGccg                         >  1:689312/3‑250 (MQ=255)                                                                                                                                                               gAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGccgc                        >  1:580600/1‑248 (MQ=255)                                                                                                                                                                   acacAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGCCGCCCTCa                   >  1:729631/1‑251 (MQ=255)                                                                                                                                                                    cacagTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCACTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGCCGCCCTCAc                  <  1:653993/247‑1 (MQ=255)                                                                                                                                                                     acagTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCa                                                                                       <  1:679021/178‑1 (MQ=255)                                                                                                                                                                      cagTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                                                                 >  1:235580/3‑170 (MQ=255)                                                                                                                                                                      cagTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaat                                               >  1:15636/3‑214 (MQ=255)                                                                                                                                                                        gTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                                                                                  >  1:387236/1‑86 (MQ=255)                                                                                                                                                                        gTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                                                                 >  1:724677/1‑168 (MQ=255)                                                                                                                                                                        gTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCa                                                                                       >  1:591838/1‑178 (MQ=255)                                                                                                                                                                        gTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGc                                                                         <  1:143763/192‑1 (MQ=255)                                                                                                                                                                        gTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaatctccatacgac                                    <  1:638303/229‑18 (MQ=255)                                                                                                                                                                        gTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGCCGCCCTCAccgc               >  1:305672/1‑248 (MQ=255)                                                                                                                                                                        gTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGCCGCCCTCAccgc               >  1:284003/1‑248 (MQ=255)                                                                                                                                                                        gTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGCCGCCCTCAccgc               >  1:890396/1‑248 (MQ=255)                                                                                                                                                                            tGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGCCGCCCTCACCGCCgg            <  1:255487/249‑1 (MQ=255)                                                                                                                                                                                  tGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGCCGCCCTCACCGCCGGTGTTATcg    <  1:316226/251‑2 (MQ=255)                                                                                                                                                                                   gccacgAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGCCGCCCTCACCGCCGGTGTTATcg    >  1:70543/5‑249 (MQ=255)                                                                                                                                                                                    ccacgAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGCCGCCCTCACCGCCGGTGTTATcgcc  <  1:291539/248‑4 (MQ=255)                                                                                                                                                                                     cacgAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaatctccatacgac                                    >  1:213671/3‑199 (MQ=255)                                                                                                                                                                                               |                                                                                                                                                                                                                                                GTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGTTTGATTATTTGCCGCCGACGGGTTATACACACCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGCTCACCCATCAGGATTTCCATTCGCCCCTGGATGCCTCCCTCACCTCCGGTGTTATCACC  >  1/1484308‑1484737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑