Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,474	G→A	intergenic (‑39846/‑157290)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,474	0	G	A	100.0%	152.6 / NA	58	intergenic (‑39846/‑157290)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/29);  total (29/29)

CCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGTTTGATTATTTGCCGCCGACGGGTTATACACACCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGCTCACCCATCAGGATTTCCATTCGCCCCTGGATGCCTCCCTCA  >  1/1484230‑1484720                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                                                               <  1:148821/251‑1 (MQ=255) cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                                                               <  1:840566/251‑1 (MQ=255) cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                                                               <  1:39040/251‑1 (MQ=255)     gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg                                                                                                                                                                                                                                                    <  1:582862/242‑1 (MQ=255)     gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                                                               <  1:381427/247‑1 (MQ=255)     gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg                                                                                                                                                                                                                                            >  1:936/1‑250 (MQ=255)     gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg                                                                                                                                                                                                                                            >  1:705772/1‑250 (MQ=255)     gCTTTTATTAGTGGCGCATTCAACCAGGATATGAGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGCACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg                                                                                                                                                                                                                                                    <  1:163371/242‑1 (MQ=255)        tttATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAAt                                                                                                                                                                                                                                             >  1:693491/1‑246 (MQ=255)                                                                               gctctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                                                                 >  1:70762/18‑250 (MQ=255)                                                                                  ctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTAtgc                                                                                                                                                              <  1:436180/237‑1 (MQ=255)                                                                                    gccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAttatg                                                                                                                                                               >  1:681245/13‑245 (MQ=255)                                                                                                          gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTg                                                                                                                                      >  1:865665/1‑251 (MQ=255)                                                                                                          gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGCAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAgcgcctt                                                                                                                                      >  1:834943/1‑248 (MQ=255)                                                                                                              gTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTgcgc                                                                                                                                   >  1:30435/1‑250 (MQ=255)                                                                                                               tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCtttttt                                                                                                                                                >  1:515774/1‑236 (MQ=255)                                                                                                               tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAg                                                                                                                                            <  1:841382/240‑1 (MQ=255)                                                                                                                    gcgcAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                                                                 >  1:826628/1‑213 (MQ=255)                                                                                                                                    catttttCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTgc                                                                                                            <  1:149503/249‑1 (MQ=255)                                                                                                                                                  gtaccataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGTGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGt                                                                                                 >  1:772783/11‑251 (MQ=255)                                                                                                                                                  gtaccataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGatta                                                                                                                 >  1:845900/11‑235 (MQ=255)                                                                                                                                                    accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGatta                                                                                                                 <  1:508408/225‑1 (MQ=255)                                                                                                                                                    accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTta                                                                                               >  1:430838/9‑251 (MQ=255)                                                                                                                                                    accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTta                                                                                               >  1:826927/9‑251 (MQ=255)                                                                                                                                                       ataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACGCAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATac                                                                                            <  1:612641/246‑1 (MQ=255)                                                                                                                                                       ataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGt                                                                                                 <  1:70541/241‑1 (MQ=255)                                                                                                                                                            ccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATacacccc                                                                                       <  1:819858/251‑4 (MQ=255)                                                                                                                                                            ccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATacac                                                                                          <  1:759106/248‑1 (MQ=255)                                                                                                                                                                 gtgCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCggct                                                                                                                       <  1:511702/214‑3 (MQ=255)                                                                                                                                                                    ccAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                                                <  1:51602/250‑1 (MQ=255)                                                                                                                                                                    ccAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                                                <  1:22678/250‑1 (MQ=255)                                                                                                                                                                        ccTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                                                <  1:384705/246‑1 (MQ=255)                                                                                                                                                                          tttGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                                                                 >  1:312476/1‑162 (MQ=255)                                                                                                                                                                          tttGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTaa                                                                         >  1:106708/1‑251 (MQ=255)                                                                                                                                                                                      gcgcagacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                                                <  1:891950/225‑1 (MQ=255)                                                                                                                                                                                      gcgcagacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTggg                                                             >  1:346796/8‑251 (MQ=255)                                                                                                                                                                                           gacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAACCACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATac                                                                                            <  1:892568/213‑1 (MQ=255)                                                                                                                                                                                             cGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtt                                                      >  1:431427/1‑251 (MQ=255)                                                                                                                                                                                               gTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtttt                                                    >  1:328909/1‑251 (MQ=255)                                                                                                                                                                                               gTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtttt                                                    >  1:555558/1‑251 (MQ=255)                                                                                                                                                                                                   cTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTc                                                <  1:513218/251‑1 (MQ=255)                                                                                                                                                                                                     gtgttttttAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCg                                              >  1:61062/5‑251 (MQ=255)                                                                                                                                                                                                       gttgtttAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTGCACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATTCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTgccgc                                                                                                         <  1:770145/186‑1 (MQ=255)                                                                                                                                                                                                                     gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAg                                                                                 >  1:394380/1‑200 (MQ=255)                                                                                                                                                                                                                     gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTc                                                >  1:678302/1‑233 (MQ=255)                                                                                                                                                                                                                     gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaat                              >  1:372824/1‑245 (MQ=255)                                                                                                                                                                                                                     gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaat                              >  1:554196/1‑245 (MQ=255)                                                                                                                                                                                                                              caacTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttgaaatctccatac                      <  1:470632/247‑15 (MQ=255)                                                                                                                                                                                                                                acTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaatctccatacgac                   <  1:600546/250‑18 (MQ=255)                                                                                                                                                                                                                                 cTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaatctccatacgac                   >  1:56549/1‑233 (MQ=255)                                                                                                                                                                                                                                        ggCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATc                                                                       <  1:577690/191‑1 (MQ=255)                                                                                                                                                                                                                                         gcctgAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACGCTGCCGGGGCTGACATTGCAGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGAt            <  1:341952/245‑1 (MQ=255)                                                                                                                                                                                                                                         gcctgAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGAt            <  1:580596/245‑1 (MQ=255)                                                                                                                                                                                                                                         gcctgAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGAt            <  1:872816/245‑1 (MQ=255)                                                                                                                                                                                                                                         gcctgAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGAt            <  1:28247/245‑1 (MQ=255)                                                                                                                                                                                                                                           ctgAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGccg        >  1:689312/3‑250 (MQ=255)                                                                                                                                                                                                                                             gAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGccgc       >  1:580600/1‑248 (MQ=255)                                                                                                                                                                                                                                                 acacAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCATTAAAATCTCCATACGACCCTGGATGCCGCCCTCa  >  1:729631/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       CCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGTTTGATTATTTGCCGCCGACGGGTTATACACACCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGCTCACCCATCAGGATTTCCATTCGCCCCTGGATGCCTCCCTCA  >  1/1484230‑1484720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑