Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,453	G→A	intergenic (‑39825/‑157311)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,453	0	G	A	100.0%	143.1 / NA	53	intergenic (‑39825/‑157311)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/27);  total (26/27)

TCTGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGTTTGATTATTTGCCGCCGACGGGTTATACACACCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGCTCACCCATCAGGAT  >  1/1484208‑1484692                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                tcggATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAg                                                                                                                                                                                                                                         <  1:879461/248‑1 (MQ=255)     aTAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGcc                                                                                                                                                                                                                                      <  1:23902/250‑1 (MQ=255)        aGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGaa                                                                                                                                                                                                                                  >  1:247708/1‑251 (MQ=255)          tCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGaaa                                                                                                                                                                                                                                 <  1:316830/250‑1 (MQ=255)             tttttGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAacac                                                                                                                                                                                                                             <  1:847608/251‑1 (MQ=255)             tttttGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAacac                                                                                                                                                                                                                             <  1:570486/251‑1 (MQ=255)                       cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                                   <  1:148821/251‑1 (MQ=255)                       cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                                   <  1:840566/251‑1 (MQ=255)                       cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                                   <  1:39040/251‑1 (MQ=255)                           gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg                                                                                                                                                                                                                        <  1:582862/242‑1 (MQ=255)                           gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                                   <  1:381427/247‑1 (MQ=255)                           gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg                                                                                                                                                                                                                >  1:936/1‑250 (MQ=255)                           gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg                                                                                                                                                                                                                >  1:705772/1‑250 (MQ=255)                           gCTTTTATTAGTGGCGCATTCAACCAGGATATGAGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGCACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg                                                                                                                                                                                                                        <  1:163371/242‑1 (MQ=255)                              tttATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAAt                                                                                                                                                                                                                 >  1:693491/1‑246 (MQ=255)                                                                                                     gctctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                                     >  1:70762/18‑250 (MQ=255)                                                                                                        ctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTAtgc                                                                                                                                  <  1:436180/237‑1 (MQ=255)                                                                                                          gccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAttatg                                                                                                                                   >  1:681245/13‑245 (MQ=255)                                                                                                                                gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTg                                                                                                          >  1:865665/1‑251 (MQ=255)                                                                                                                                gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGCAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAgcgcctt                                                                                                          >  1:834943/1‑248 (MQ=255)                                                                                                                                    gTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTgcgc                                                                                                       >  1:30435/1‑250 (MQ=255)                                                                                                                                     tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCtttttt                                                                                                                    >  1:515774/1‑236 (MQ=255)                                                                                                                                     tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAg                                                                                                                <  1:841382/240‑1 (MQ=255)                                                                                                                                          gcgcAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                                     >  1:826628/1‑213 (MQ=255)                                                                                                                                                          catttttCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTgc                                                                                <  1:149503/249‑1 (MQ=255)                                                                                                                                                                        gtaccataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGTGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGt                                                                     >  1:772783/11‑251 (MQ=255)                                                                                                                                                                        gtaccataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGatta                                                                                     >  1:845900/11‑235 (MQ=255)                                                                                                                                                                          accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGatta                                                                                     <  1:508408/225‑1 (MQ=255)                                                                                                                                                                          accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTta                                                                   >  1:826927/9‑251 (MQ=255)                                                                                                                                                                          accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTta                                                                   >  1:430838/9‑251 (MQ=255)                                                                                                                                                                             ataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACGCAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATac                                                                <  1:612641/246‑1 (MQ=255)                                                                                                                                                                             ataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGt                                                                     <  1:70541/241‑1 (MQ=255)                                                                                                                                                                                  ccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATacacccc                                                           <  1:819858/251‑4 (MQ=255)                                                                                                                                                                                  ccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATacac                                                              <  1:759106/248‑1 (MQ=255)                                                                                                                                                                                       gtgCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCggct                                                                                           <  1:511702/214‑3 (MQ=255)                                                                                                                                                                                          ccAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                    <  1:22678/250‑1 (MQ=255)                                                                                                                                                                                          ccAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                    <  1:51602/250‑1 (MQ=255)                                                                                                                                                                                              ccTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                    <  1:384705/246‑1 (MQ=255)                                                                                                                                                                                                tttGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                                     >  1:312476/1‑162 (MQ=255)                                                                                                                                                                                                tttGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTaa                                             >  1:106708/1‑251 (MQ=255)                                                                                                                                                                                                            gcgcagacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                                    <  1:891950/225‑1 (MQ=255)                                                                                                                                                                                                            gcgcagacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTggg                                 >  1:346796/8‑251 (MQ=255)                                                                                                                                                                                                                 gacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAACCACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATac                                                                <  1:892568/213‑1 (MQ=255)                                                                                                                                                                                                                   cGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtt                          >  1:431427/1‑251 (MQ=255)                                                                                                                                                                                                                     gTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtttt                        >  1:328909/1‑251 (MQ=255)                                                                                                                                                                                                                     gTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtttt                        >  1:555558/1‑251 (MQ=255)                                                                                                                                                                                                                         cTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTc                    <  1:513218/251‑1 (MQ=255)                                                                                                                                                                                                                           gtgttttttAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCg                  >  1:61062/5‑251 (MQ=255)                                                                                                                                                                                                                             gttgtttAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTGCACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATTCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTgccgc                                                                             <  1:770145/186‑1 (MQ=255)                                                                                                                                                                                                                                           gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAg                                                     >  1:394380/1‑200 (MQ=255)                                                                                                                                                                                                                                           gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTc                    >  1:678302/1‑233 (MQ=255)                                                                                                                                                                                                                                           gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaat  >  1:554196/1‑245 (MQ=255)                                                                                                                                                                                                                                           gCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGTTCGCCCAttaaaat  >  1:372824/1‑245 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                TCTGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGTTTGATTATTTGCCGCCGACGGGTTATACACACCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTCCGGCTCACCCATCAGGAT  >  1/1484208‑1484692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑