Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,429	C→T	intergenic (‑39801/‑157335)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,429	0	C	T	100.0%	159.1 / NA	57	intergenic (‑39801/‑157335)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/35);  total (22/35)

CTTTTTTATAGCTGTCTAAATCCAGCTCTGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGTTTGATTATTTGCCGCCGACGGGTTATACACACCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTC  >  1/1484182‑1484674                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      cTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTAATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGtttttt                                                                                                                                                                                                                                                  <  1:724342/250‑1 (MQ=255)  ttttttGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAg                                                                                                                                                                                                                                                <  1:432391/251‑1 (MQ=255)    ttttGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAggg                                                                                                                                                                                                                                              <  1:632450/251‑1 (MQ=255)            cTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGtt                                                                                                                                                                                                                                      <  1:490477/251‑1 (MQ=255)              gTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTtat                                                                                                                                                                                                                                    >  1:876568/1‑251 (MQ=255)                cTAAATCCAGCTCGGATAAGTTTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                                                                                                                  <  1:870074/251‑1 (MQ=255)                cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                                                                                                                  <  1:510612/251‑1 (MQ=255)                cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                                                                                                                  <  1:487576/251‑1 (MQ=255)                cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                                                                                                                  <  1:241348/251‑1 (MQ=255)                 tAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATagc                                                                                                                                                                                                                                 <  1:11148/251‑1 (MQ=255)                           tcggATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAg                                                                                                                                                                                                                       <  1:879461/248‑1 (MQ=255)                               aTAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGcc                                                                                                                                                                                                                    <  1:23902/250‑1 (MQ=255)                                  aGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGaa                                                                                                                                                                                                                >  1:247708/1‑251 (MQ=255)                                    tCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGaaa                                                                                                                                                                                                               <  1:316830/250‑1 (MQ=255)                                       tttttGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAacac                                                                                                                                                                                                           <  1:847608/251‑1 (MQ=255)                                       tttttGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAacac                                                                                                                                                                                                           <  1:570486/251‑1 (MQ=255)                                                 cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                 <  1:840566/251‑1 (MQ=255)                                                 cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                 <  1:39040/251‑1 (MQ=255)                                                 cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                 <  1:148821/251‑1 (MQ=255)                                                     gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCg                                                                                                                                                                                                                                        <  1:78965/208‑1 (MQ=255)                                                     gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg                                                                                                                                                                                                      <  1:582862/242‑1 (MQ=255)                                                     gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                                                 <  1:381427/247‑1 (MQ=255)                                                     gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg                                                                                                                                                                                              >  1:705772/1‑250 (MQ=255)                                                     gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg                                                                                                                                                                                              >  1:936/1‑250 (MQ=255)                                                     gCTTTTATTAGTGGCGCATTCAACCAGGATATGAGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGCACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg                                                                                                                                                                                                      <  1:163371/242‑1 (MQ=255)                                                        tttATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAAt                                                                                                                                                                                               >  1:693491/1‑246 (MQ=255)                                                                                                                               gctctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                   >  1:70762/18‑250 (MQ=255)                                                                                                                                  ctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTAtgc                                                                                                                <  1:436180/237‑1 (MQ=255)                                                                                                                                    gccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAttatg                                                                                                                 >  1:681245/13‑245 (MQ=255)                                                                                                                                                          gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTg                                                                                        >  1:865665/1‑251 (MQ=255)                                                                                                                                                          gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGCAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAgcgcctt                                                                                        >  1:834943/1‑248 (MQ=255)                                                                                                                                                              gTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTgcgc                                                                                     >  1:30435/1‑250 (MQ=255)                                                                                                                                                               tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCtttttt                                                                                                  >  1:515774/1‑236 (MQ=255)                                                                                                                                                               tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAg                                                                                              <  1:841382/240‑1 (MQ=255)                                                                                                                                                                    gcgcAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                   >  1:826628/1‑213 (MQ=255)                                                                                                                                                                                    catttttCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTgc                                                              <  1:149503/249‑1 (MQ=255)                                                                                                                                                                                                  gtaccataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGTGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGt                                                   >  1:772783/11‑251 (MQ=255)                                                                                                                                                                                                  gtaccataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGatta                                                                   >  1:845900/11‑235 (MQ=255)                                                                                                                                                                                                    accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGatta                                                                   <  1:508408/225‑1 (MQ=255)                                                                                                                                                                                                    accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTta                                                 >  1:430838/9‑251 (MQ=255)                                                                                                                                                                                                    accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTta                                                 >  1:826927/9‑251 (MQ=255)                                                                                                                                                                                                       ataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACGCAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATac                                              <  1:612641/246‑1 (MQ=255)                                                                                                                                                                                                       ataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGt                                                   <  1:70541/241‑1 (MQ=255)                                                                                                                                                                                                            ccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATacacccc                                         <  1:819858/251‑4 (MQ=255)                                                                                                                                                                                                            ccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATacac                                            <  1:759106/248‑1 (MQ=255)                                                                                                                                                                                                                 gtgCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCggct                                                                         <  1:511702/214‑3 (MQ=255)                                                                                                                                                                                                                    ccAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                  <  1:22678/250‑1 (MQ=255)                                                                                                                                                                                                                    ccAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                  <  1:51602/250‑1 (MQ=255)                                                                                                                                                                                                                        ccTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                  <  1:384705/246‑1 (MQ=255)                                                                                                                                                                                                                          tttGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                                                   >  1:312476/1‑162 (MQ=255)                                                                                                                                                                                                                          tttGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTaa                           >  1:106708/1‑251 (MQ=255)                                                                                                                                                                                                                                      gcgcagacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc                                  <  1:891950/225‑1 (MQ=255)                                                                                                                                                                                                                                      gcgcagacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTggg               >  1:346796/8‑251 (MQ=255)                                                                                                                                                                                                                                           gacGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAACCACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATac                                              <  1:892568/213‑1 (MQ=255)                                                                                                                                                                                                                                             cGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtt        >  1:431427/1‑251 (MQ=255)                                                                                                                                                                                                                                               gTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtttt      >  1:328909/1‑251 (MQ=255)                                                                                                                                                                                                                                               gTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCtttt      >  1:555558/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTc  <  1:513218/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      CTTTTTTATAGCTGTCTAAATCCAGCTCTGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGTTTGATTATTTGCCGCCGACGGGTTATACACACCTTTAAGCAGATTAATCAGCATTTGGGTCGGCTTTTGGTC  >  1/1484182‑1484674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑