Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,403	T→A	intergenic (‑39775/‑157361)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,403	0	T	A	100.0%	161.1 / NA	59	intergenic (‑39775/‑157361)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/39);  total (20/39)

CATCGAAAAGCGTATCTGCCGCTTTTTTATAGCTGTCTAAATCCAGCTCTGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGTTTGATTATTTGCCGCCGACGGGTTATACACACCTTTAAGCAGATTAA  >  1/1484161‑1484649                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       cgtCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGgcg                                                                                                                                                                                                                                              >  1:80101/3‑250 (MQ=255)      aaaaGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCagacg                                                                                                                                                                                                                                        <  1:159898/251‑5 (MQ=255)      aaaaGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCagac                                                                                                                                                                                                                                         <  1:193367/250‑4 (MQ=255)                   ccGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCtgtgttt                                                                                                                                                                                                                            <  1:410924/250‑4 (MQ=255)                   ccGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCtgtgt                                                                                                                                                                                                                              <  1:528457/248‑2 (MQ=255)                      cTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTAATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGtttttt                                                                                                                                                                                                                         <  1:724342/250‑1 (MQ=255)                       ttttttGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAg                                                                                                                                                                                                                       <  1:432391/251‑1 (MQ=255)                         ttttGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAggg                                                                                                                                                                                                                     <  1:632450/251‑1 (MQ=255)                                 cTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGtt                                                                                                                                                                                                             <  1:490477/251‑1 (MQ=255)                                   gTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGc                                                                                                                                                                                                                                   <  1:385703/227‑1 (MQ=255)                                   gTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTtat                                                                                                                                                                                                           >  1:876568/1‑251 (MQ=255)                                    tCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCagacg                                                                                                                                                                                                                                        >  1:263204/1‑217 (MQ=255)                                     cTAAATCCAGCTCGGATAAGTTTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                                                                                         <  1:870074/251‑1 (MQ=255)                                     cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                                                                                         <  1:510612/251‑1 (MQ=255)                                     cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                                                                                         <  1:487576/251‑1 (MQ=255)                                     cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                                                                                         <  1:241348/251‑1 (MQ=255)                                      tAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATagc                                                                                                                                                                                                        <  1:11148/251‑1 (MQ=255)                                                tcggATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAg                                                                                                                                                                                              <  1:879461/248‑1 (MQ=255)                                                    aTAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGcc                                                                                                                                                                                           <  1:23902/250‑1 (MQ=255)                                                       aGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGaa                                                                                                                                                                                       >  1:247708/1‑251 (MQ=255)                                                         tCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGaaa                                                                                                                                                                                      <  1:316830/250‑1 (MQ=255)                                                            tttttGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAacac                                                                                                                                                                                  <  1:570486/251‑1 (MQ=255)                                                            tttttGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAacac                                                                                                                                                                                  <  1:847608/251‑1 (MQ=255)                                                                      cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                        <  1:840566/251‑1 (MQ=255)                                                                      cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                        <  1:39040/251‑1 (MQ=255)                                                                      cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                        <  1:148821/251‑1 (MQ=255)                                                                          gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGc                                                                                                                                                                                                                                   <  1:107733/188‑1 (MQ=255)                                                                          gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCg                                                                                                                                                                                                               <  1:78965/208‑1 (MQ=255)                                                                          gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg                                                                                                                                                                             <  1:582862/242‑1 (MQ=255)                                                                          gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                                                                                        <  1:381427/247‑1 (MQ=255)                                                                          gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg                                                                                                                                                                     >  1:705772/1‑250 (MQ=255)                                                                          gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg                                                                                                                                                                     >  1:936/1‑250 (MQ=255)                                                                          gCTTTTATTAGTGGCGCATTCAACCAGGATATGAGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGCACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg                                                                                                                                                                             <  1:163371/242‑1 (MQ=255)                                                                             tttATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAAt                                                                                                                                                                      >  1:693491/1‑246 (MQ=255)                                                                                                                                                    gctctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                          >  1:70762/18‑250 (MQ=255)                                                                                                                                                       ctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTAtgc                                                                                       <  1:436180/237‑1 (MQ=255)                                                                                                                                                         gccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAttatg                                                                                        >  1:681245/13‑245 (MQ=255)                                                                                                                                                                               gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTg                                                               >  1:865665/1‑251 (MQ=255)                                                                                                                                                                               gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGCAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAgcgcctt                                                               >  1:834943/1‑248 (MQ=255)                                                                                                                                                                                   gTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTgcgc                                                            >  1:30435/1‑250 (MQ=255)                                                                                                                                                                                    tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCtttttt                                                                         >  1:515774/1‑236 (MQ=255)                                                                                                                                                                                    tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAg                                                                     <  1:841382/240‑1 (MQ=255)                                                                                                                                                                                         gcgcAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                          >  1:826628/1‑213 (MQ=255)                                                                                                                                                                                                         catttttCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTgc                                     <  1:149503/249‑1 (MQ=255)                                                                                                                                                                                                                       gtaccataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGTGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGt                          >  1:772783/11‑251 (MQ=255)                                                                                                                                                                                                                       gtaccataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGatta                                          >  1:845900/11‑235 (MQ=255)                                                                                                                                                                                                                         accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGatta                                          <  1:508408/225‑1 (MQ=255)                                                                                                                                                                                                                         accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTta                        >  1:430838/9‑251 (MQ=255)                                                                                                                                                                                                                         accataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTta                        >  1:826927/9‑251 (MQ=255)                                                                                                                                                                                                                            ataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACGCAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATac                     <  1:612641/246‑1 (MQ=255)                                                                                                                                                                                                                            ataccccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGt                          <  1:70541/241‑1 (MQ=255)                                                                                                                                                                                                                                 ccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATacacccc                <  1:819858/251‑4 (MQ=255)                                                                                                                                                                                                                                 ccATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATacac                   <  1:759106/248‑1 (MQ=255)                                                                                                                                                                                                                                      gtgCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCggct                                                <  1:511702/214‑3 (MQ=255)                                                                                                                                                                                                                                         ccAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc         <  1:51602/250‑1 (MQ=255)                                                                                                                                                                                                                                         ccAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc         <  1:22678/250‑1 (MQ=255)                                                                                                                                                                                                                                             ccTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGc         <  1:384705/246‑1 (MQ=255)                                                                                                                                                                                                                                               tttGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                                                                          >  1:312476/1‑162 (MQ=255)                                                                                                                                                                                                                                               tttGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGCTTGATTATTTGCCGCCGACGGGTTATACACCCCTTTAAGCAGATTaa  >  1:106708/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       CATCGAAAAGCGTATCTGCCGCTTTTTTATAGCTGTCTAAATCCAGCTCTGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGCCCGCGCCCGGTTTGATTATTTGCCGCCGACGGGTTATACACACCTTTAAGCAGATTAA  >  1/1484161‑1484649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑