Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,353	G→C	intergenic (‑39725/‑157411)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,353	0	G	C	100.0%	206.1 / NA	75	intergenic (‑39725/‑157411)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (31/44);  total (31/44)

ATAAAATCTTTAATGGAGGTTTGCCGGTTGTAGCGGATACAGAGGCCAAATCCCTCATCGAAAAGCGTATCTGCCGCTTTTTTATAGCTGTCTAAATCCAGCTCTGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGC  >  1/1484106‑1484591                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ataaACTCCTTAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGCGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTACTTTTGCGATTGTCATTGCGCAGCACGAt                                                                                                                                                                                                                                             >  1:721069/1‑251 (MQ=255)   aaactccttAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGAttt                                                                                                                                                                                                                                           <  1:599979/244‑1 (MQ=255)        ccttAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTTCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTgcat                                                                                                                                                                                                                                      >  1:842950/3‑248 (MQ=255)        ccttAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGGCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTgcat                                                                                                                                                                                                                                      <  1:862738/249‑4 (MQ=255)                  ggTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCggggt                                                                                                                                                                                                                            >  1:695765/1‑248 (MQ=255)                   gTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCggggt                                                                                                                                                                                                                            <  1:889059/250‑4 (MQ=255)                    tttGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtac                                                                                                                                                                                                                          <  1:262542/251‑1 (MQ=255)                        ccgattGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTGCCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccat                                                                                                                                                                                                                       <  1:389850/246‑1 (MQ=255)                        ccgattGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccat                                                                                                                                                                                                                       <  1:730376/246‑1 (MQ=255)                          gattGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatac                                                                                                                                                                                                                     >  1:386890/3‑250 (MQ=255)                           attGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccataccc                                                                                                                                                                                                                   <  1:644129/250‑2 (MQ=255)                           attGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacc                                                                                                                                                                                                                    <  1:861551/249‑1 (MQ=255)                            ttGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacccc                                                                                                                                                                                                                  <  1:613288/251‑3 (MQ=255)                            ttGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacccc                                                                                                                                                                                                                  <  1:578759/251‑3 (MQ=255)                              gTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccataccccat                                                                                                                                                                                                                >  1:427959/1‑247 (MQ=255)                               tAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccat                                                                                                                                                                                                                       >  1:812226/1‑243 (MQ=255)                                aGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGAt                                                                                                                                                                                                                                             >  1:226205/1‑220 (MQ=255)                                aGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccataccccattg                                                                                                                                                                                                              >  1:847849/1‑245 (MQ=255)                                       acacaacccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatac                                                                                                                                                                                                                     >  1:838638/11‑237 (MQ=255)                                       acacaacccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAg                                                                                                                                                                                                     >  1:162616/11‑250 (MQ=255)                                         acaacccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCt                                                                                                                                                                                                  <  1:310029/243‑1 (MQ=255)                                            acccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTtg                                                                                                                                                                                               >  1:259196/6‑251 (MQ=255)                                            acccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTtg                                                                                                                                                                                               >  1:852080/6‑251 (MQ=255)                                             cccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCggg                                                                                                                                                                                                                              <  1:683668/218‑2 (MQ=255)                                             cccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccataccccattgtgc                                                                                                                                                                                                           <  1:356017/237‑10 (MQ=255)                                                        cgtCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGgcg                                                                                                                                                                                    >  1:80101/3‑250 (MQ=255)                                                         gtCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacc                                                                                                                                                                                                                    >  1:17939/2‑220 (MQ=255)                                                             aaaaGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCagacg                                                                                                                                                                              <  1:159898/251‑5 (MQ=255)                                                             aaaaGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCagac                                                                                                                                                                               <  1:193367/250‑4 (MQ=255)                                                                  tgTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacccc                                                                                                                                                                                                                  >  1:881058/2‑211 (MQ=255)                                                                          ccGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCtgtgttt                                                                                                                                                                  <  1:410924/250‑4 (MQ=255)                                                                          ccGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCtgtgt                                                                                                                                                                    <  1:528457/248‑2 (MQ=255)                                                                             cTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTAATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGtttttt                                                                                                                                                               <  1:724342/250‑1 (MQ=255)                                                                              ttttttGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAg                                                                                                                                                             <  1:432391/251‑1 (MQ=255)                                                                                ttttGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtac                                                                                                                                                                                                                          >  1:895310/1‑191 (MQ=255)                                                                                ttttGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAggg                                                                                                                                                           <  1:632450/251‑1 (MQ=255)                                                                                        cTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGtt                                                                                                                                                   <  1:490477/251‑1 (MQ=255)                                                                                          gTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccataccc                                                                                                                                                                                                                   <  1:130852/188‑2 (MQ=255)                                                                                          gTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGc                                                                                                                                                                         <  1:385703/227‑1 (MQ=255)                                                                                          gTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTtat                                                                                                                                                 >  1:876568/1‑251 (MQ=255)                                                                                           tCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCagacg                                                                                                                                                                              >  1:263204/1‑217 (MQ=255)                                                                                            cTAAATCCAGCTCGGATAAGTTTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                               <  1:870074/251‑1 (MQ=255)                                                                                            cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                               <  1:510612/251‑1 (MQ=255)                                                                                            cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                               <  1:487576/251‑1 (MQ=255)                                                                                            cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                                                                                                                               <  1:241348/251‑1 (MQ=255)                                                                                             tAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATagc                                                                                                                                              <  1:11148/251‑1 (MQ=255)                                                                                                       tcggATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAg                                                                                                                                    <  1:879461/248‑1 (MQ=255)                                                                                                        cggATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccat                                                                                                                                                                                                                       >  1:308402/3‑170 (MQ=255)                                                                                                         ggATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacccc                                                                                                                                                                                                                  >  1:130671/2‑172 (MQ=255)                                                                                                           aTAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGcc                                                                                                                                 <  1:23902/250‑1 (MQ=255)                                                                                                              aGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGaa                                                                                                                             >  1:247708/1‑251 (MQ=255)                                                                                                                tCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGaaa                                                                                                                            <  1:316830/250‑1 (MQ=255)                                                                                                                   tttttGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAacac                                                                                                                        <  1:847608/251‑1 (MQ=255)                                                                                                                   tttttGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAacac                                                                                                                        <  1:570486/251‑1 (MQ=255)                                                                                                                             cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                              <  1:148821/251‑1 (MQ=255)                                                                                                                             cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                              <  1:840566/251‑1 (MQ=255)                                                                                                                             cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                              <  1:39040/251‑1 (MQ=255)                                                                                                                                 gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTtgag                                                                                                                                                                                             <  1:212888/168‑3 (MQ=255)                                                                                                                                 gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGc                                                                                                                                                                         <  1:107733/188‑1 (MQ=255)                                                                                                                                 gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCg                                                                                                                                                     <  1:78965/208‑1 (MQ=255)                                                                                                                                 gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg                                                                                                                   <  1:582862/242‑1 (MQ=255)                                                                                                                                 gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa                                                                                                              <  1:381427/247‑1 (MQ=255)                                                                                                                                 gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg                                                                                                           >  1:936/1‑250 (MQ=255)                                                                                                                                 gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg                                                                                                           >  1:705772/1‑250 (MQ=255)                                                                                                                                 gCTTTTATTAGTGGCGCATTCAACCAGGATATGAGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGCACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg                                                                                                                   <  1:163371/242‑1 (MQ=255)                                                                                                                                    tttATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAAt                                                                                                            >  1:693491/1‑246 (MQ=255)                                                                                                                                                                                                           gctctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                >  1:70762/18‑250 (MQ=255)                                                                                                                                                                                                              ctgccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTAtgc                             <  1:436180/237‑1 (MQ=255)                                                                                                                                                                                                                gccgttacccccttAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAttatg                              >  1:681245/13‑245 (MQ=255)                                                                                                                                                                                                                                      gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTg     >  1:865665/1‑251 (MQ=255)                                                                                                                                                                                                                                      gATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGCAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAgcgcctt     >  1:834943/1‑248 (MQ=255)                                                                                                                                                                                                                                          gTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAGCGCTTgcgc  >  1:30435/1‑250 (MQ=255)                                                                                                                                                                                                                                           tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCtttttt               >  1:515774/1‑236 (MQ=255)                                                                                                                                                                                                                                           tCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTATTATGCTGCCCGTCTTTTTTAAAg           <  1:841382/240‑1 (MQ=255)                                                                                                                                                                                                                                                gcgcAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATGCCACGAAAGCCGGGGATAGTCTCATCTGTTGCTACACTGCCGGGGCTGACATTGCCGGGTCGGAAAAATTTAtta                                >  1:826628/1‑213 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ATAAAATCTTTAATGGAGGTTTGCCGGTTGTAGCGGATACAGAGGCCAAATCCCTCATCGAAAAGCGTATCTGCCGCTTTTTTATAGCTGTCTAAATCCAGCTCTGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATGCCGCGAAAGCCCGGGATAGTCTCATCTGTTGCCACATTACCGGGGCTGACATTGCCGGGTCGGAAAAACTTATTGTGCTGCCCGTCTTTTTTAAAGCGCTTGCGC  >  1/1484106‑1484591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑