Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,484,309	T→C	intergenic (‑39681/‑157455)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,484,309	0	T	C	100.0%	213.9 / NA	74	intergenic (‑39681/‑157455)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/45);  total (29/45)

ACAATTTGCTTAATAAAATCTTTAATGGAGGTTTGCCGGTTGTAGCGGATACAGAGGCCAAATCCCTCATCGAAAAGCGTATCTGCCGCTTTTTTATAGCTGTCTAAATCCAGCTCTGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATG  >  1/1484094‑1484486                                                                                                                                                                                                                        |                                                                                                                                                                                  acgaTTTGCTTAATAAACTCCTTAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCAtt                                                                                                                                                >  1:666102/4‑251 (MQ=255) acgaTTTGCTTAATAAACTCCTTAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCAtt                                                                                                                                                <  1:493187/248‑1 (MQ=255)    aTTTGCTTAATAAACTCCTTAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTgc                                                                                                                                              <  1:29773/250‑1 (MQ=255)     tttGCTTAATAAACTCCTTAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTgcgc                                                                                                                                            <  1:235553/251‑1 (MQ=255)      ttGCTTAATAAACTCCTTAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCgca                                                                                                                                           >  1:60118/1‑251 (MQ=255)           taataaACTCCTTAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCgcagcacg                                                                                                                                      >  1:359268/1‑249 (MQ=255)             ataaACTCCTTAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGCGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTACTTTTGCGATTGTCATTGCGCAGCACGAt                                                                                                                                    >  1:721069/1‑251 (MQ=255)               aaactccttAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGAttt                                                                                                                                  <  1:599979/244‑1 (MQ=255)                    ccttAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTTCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTgcat                                                                                                                             >  1:842950/3‑248 (MQ=255)                    ccttAATTGAGGTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGGCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTgcat                                                                                                                             <  1:862738/249‑4 (MQ=255)                              ggTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCggggt                                                                                                                   >  1:695765/1‑248 (MQ=255)                               gTTTGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCggggt                                                                                                                   <  1:889059/250‑4 (MQ=255)                                tttGCCGATTGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtac                                                                                                                 <  1:262542/251‑1 (MQ=255)                                    ccgattGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTGCCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccat                                                                                                              <  1:389850/246‑1 (MQ=255)                                    ccgattGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccat                                                                                                              <  1:730376/246‑1 (MQ=255)                                      gattGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatac                                                                                                            >  1:386890/3‑250 (MQ=255)                                       attGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccataccc                                                                                                          <  1:644129/250‑2 (MQ=255)                                       attGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacc                                                                                                           <  1:861551/249‑1 (MQ=255)                                        ttGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacccc                                                                                                         <  1:578759/251‑3 (MQ=255)                                        ttGTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacccc                                                                                                         <  1:613288/251‑3 (MQ=255)                                          gTAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccataccccat                                                                                                       >  1:427959/1‑247 (MQ=255)                                           tAGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccat                                                                                                              >  1:812226/1‑243 (MQ=255)                                            aGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGAt                                                                                                                                    >  1:226205/1‑220 (MQ=255)                                            aGCGGATACACAACCCGAATCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccataccccattg                                                                                                     >  1:847849/1‑245 (MQ=255)                                                  tacacaacccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTgcgc                                                                                                                                            >  1:334947/12‑206 (MQ=255)                                                   acacaacccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatac                                                                                                            >  1:838638/11‑237 (MQ=255)                                                   acacaacccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAg                                                                                            >  1:162616/11‑250 (MQ=255)                                                     acaacccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCt                                                                                         <  1:310029/243‑1 (MQ=255)                                                        acccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTgcgc                                                                                                                                            >  1:272132/6‑200 (MQ=255)                                                        acccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTtg                                                                                      >  1:852080/6‑251 (MQ=255)                                                        acccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTtg                                                                                      >  1:259196/6‑251 (MQ=255)                                                         cccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCggg                                                                                                                     <  1:683668/218‑2 (MQ=255)                                                         cccgaaTCCCTCGTCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccataccccattgtgc                                                                                                  <  1:356017/237‑10 (MQ=255)                                                                    cgtCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGgcg                                                                           >  1:80101/3‑250 (MQ=255)                                                                     gtCGAAAAGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacc                                                                                                           >  1:17939/2‑220 (MQ=255)                                                                         aaaaGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCagacg                                                                     <  1:159898/251‑5 (MQ=255)                                                                         aaaaGTGTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCagac                                                                      <  1:193367/250‑4 (MQ=255)                                                                              tgTATCAGCCGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacccc                                                                                                         >  1:881058/2‑211 (MQ=255)                                                                                      ccGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCtgtgttt                                                         <  1:410924/250‑4 (MQ=255)                                                                                      ccGCTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCtgtgt                                                           <  1:528457/248‑2 (MQ=255)                                                                                         cTTTTTTGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTAATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGtttttt                                                      <  1:724342/250‑1 (MQ=255)                                                                                          ttttttGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAg                                                    <  1:432391/251‑1 (MQ=255)                                                                                            ttttGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtac                                                                                                                 >  1:895310/1‑191 (MQ=255)                                                                                            ttttGTAGCTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAggg                                                  <  1:632450/251‑1 (MQ=255)                                                                                                    cTGTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGtt                                          <  1:490477/251‑1 (MQ=255)                                                                                                      gTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccataccc                                                                                                          <  1:130852/188‑2 (MQ=255)                                                                                                      gTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGc                                                                <  1:385703/227‑1 (MQ=255)                                                                                                      gTCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTtat                                        >  1:876568/1‑251 (MQ=255)                                                                                                       tCTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCagacg                                                                     >  1:263204/1‑217 (MQ=255)                                                                                                        cTAAATCCAGCTCGGATAAGTTTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                      <  1:870074/251‑1 (MQ=255)                                                                                                        cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                      <  1:510612/251‑1 (MQ=255)                                                                                                        cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                      <  1:487576/251‑1 (MQ=255)                                                                                                        cTAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATag                                      <  1:241348/251‑1 (MQ=255)                                                                                                         tAAATCCAGCTCGGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATagc                                     <  1:11148/251‑1 (MQ=255)                                                                                                                   tcggATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAg                           <  1:879461/248‑1 (MQ=255)                                                                                                                    cggATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccat                                                                                                              >  1:308402/3‑170 (MQ=255)                                                                                                                     ggATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGtaccatacccc                                                                                                         >  1:130671/2‑172 (MQ=255)                                                                                                                       aTAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGcc                        <  1:23902/250‑1 (MQ=255)                                                                                                                          aGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGaa                    >  1:247708/1‑251 (MQ=255)                                                                                                                            tCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGaaa                   <  1:316830/250‑1 (MQ=255)                                                                                                                               tttttGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAacac               <  1:847608/251‑1 (MQ=255)                                                                                                                               tttttGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAacac               <  1:570486/251‑1 (MQ=255)                                                                                                                                         cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa     <  1:840566/251‑1 (MQ=255)                                                                                                                                         cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa     <  1:39040/251‑1 (MQ=255)                                                                                                                                         cccAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa     <  1:148821/251‑1 (MQ=255)                                                                                                                                             gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTtgag                                                                                    <  1:212888/168‑3 (MQ=255)                                                                                                                                             gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGc                                                                <  1:107733/188‑1 (MQ=255)                                                                                                                                             gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCg                                            <  1:78965/208‑1 (MQ=255)                                                                                                                                             gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg          <  1:582862/242‑1 (MQ=255)                                                                                                                                             gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACa     <  1:381427/247‑1 (MQ=255)                                                                                                                                             gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg  >  1:936/1‑250 (MQ=255)                                                                                                                                             gCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAATg  >  1:705772/1‑250 (MQ=255)                                                                                                                                             gCTTTTATTAGTGGCGCATTCAACCAGGATATGAGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGCACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAg          <  1:163371/242‑1 (MQ=255)                                                                                                                                                tttATTAGTGGCGCATTCAACCAGGATATGTGCGGGGTTCATCGCGTGGATCTCACGTAAATTTTCTTCCTGCTCTGCCGTTACCCCCTTAATTTTGAGATTGTCATTGCGCAGCACGATTTTGCATTTTTCGGGGTACCATACC‑‑‑CCATTGTGCCAGCCTTTGAGGGCACGGCGCAGACGGTAGCTGTGTTTTTTAGGGTAAGCGTTATAGCAACTGATAAGGCCTGAAAACACAGTAGTGACAAt   >  1:693491/1‑246 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                  ACAATTTGCTTAATAAAATCTTTAATGGAGGTTTGCCGGTTGTAGCGGATACAGAGGCCAAATCCCTCATCGAAAAGCGTATCTGCCGCTTTTTTATAGCTGTCTAAATCCAGCTCTGATAAGTCTTTTTTGCCGCCCCAGCTTTTATTAGTGGCGCATTCAACCAGGATATGTGCCGGGTTCATGGCGTGGATTTGGCGTACATTTTGTTCTTGTTCGGCTGTCAAACCTTTAATTTTAAGATTGTCATTGCGCAGCAGGATTTTGGCTTTTTCGGCGTACCATACCACGCCATTGTGCCAGCCTTTGTGGGCACGGCGCACCCGGTAGCTGTGCTTTTTAGGGTATGCGTTATAGCAGCTGATAAGGCCCGAAAACACGGTAGTGACAATG  >  1/1484094‑1484486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑