Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,483,424	G→A	intergenic (‑38796/‑158340)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,483,424	0	G	A	100.0%	95.1 / NA	37	intergenic (‑38796/‑158340)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/13);  total (24/13)

GCCCGCATCAACTATCAAGTCATAGCCGACAGAAAGTGCAGTCGGTTGCGCGCCCAAGCTCCAGACAAAACAGTCGGTCGGCTTGATAAATGCGCGCTCCGCGTCCGATAGCACAAGCGGGAGTACGTGATATGGCACCTCAAACAAG‑CGCGCGGAATCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAATATCGCGGT  >  1/1483340‑1483668                                                                                     |                                                                                                                                                                                                                                                       acccacattaaccagcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCa                                                                                 <  1:851526/232‑1 (MQ=255)             cagcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCACGTCGcc                                                                      >  1:885890/8‑250 (MQ=255)               gcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCCTGCCGGTAACATCCTGCAAGCACGGCGCcac‑a                                                                  >  1:123973/6‑251 (MQ=255)               gcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAAATCCTGCCAGCACGTCGCcac‑a                                                                  >  1:336286/6‑251 (MQ=255)                catatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCATGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCcac                                                                    <  1:431013/245‑1 (MQ=255)                  tatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAgcgg                                                                                                         >  1:87667/3‑210 (MQ=255)                  tatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAAATCCTGCAAGCACCTCGCCCC‑AAt                                                                >  1:493069/3‑250 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAc                                                                                         <  1:587443/224‑1 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCCC‑AAt                                                                >  1:792839/2‑249 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCACGTCGCCCC‑AAt                                                                >  1:330367/2‑249 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCCTAAACATCCTTCCAGCACGTCGCcaccaac                                                                >  1:685481/2‑246 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCAAACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCAAGCCGTAAACATCCTGCAAGCa                                                                             >  1:114049/2‑237 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGagtag                                                                                                                >  1:715757/1‑201 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTa                                                                                                             <  1:7335/204‑1 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCCTTGCGTGAACATCCTGCAAggccggctccccaaact                                                               >  1:429749/1‑234 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCACGTCGCCAC‑AAtct                                                              >  1:534190/1‑250 (MQ=255)                         gcccaccgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCa                                                                             <  1:251048/224‑1 (MQ=255)                             accgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGGATGCCGTAAAAATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCCCGtt                                                    >  1:616503/4‑251 (MQ=255)                              ccgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTg                                                   <  1:736308/249‑1 (MQ=255)                              ccgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAAAACCTGCAAGCACGTCGCCAC‑AATCTCGCCCCCGTTg                                                   >  1:224377/3‑251 (MQ=255)                                 aaagcgctgTCGGCTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTg                                                                                                                     <  1:446435/175‑1 (MQ=255)                                         gTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGCGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGTCGTAAACATCCTGCAAGAACGGCGACACAAATC‑CGCCCCCGTTTCCGTTTTTaca                                        >  1:507157/1‑249 (MQ=255)                                             gTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCCAGCACGTCGCCCC‑AATCTCGCCCCCGTTGCCGTTTTTAAGCTc                                     >  1:524924/1‑250 (MQ=255)                                                   cgcCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTgtgt                                                                                                                       >  1:750627/1‑163 (MQ=255)                                                     cccAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAg                                                                                                                                         <  1:650996/143‑1 (MQ=255)                                                     cccAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AAACTCGCCCCCGTTGCCGGTTTTAAGCTCCCCAAccc                             >  1:575099/1‑248 (MQ=255)                                                          gCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAac                        <  1:270186/250‑1 (MQ=255)                                                          gCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAac                        <  1:294858/250‑1 (MQ=255)                                                          gCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAAAATCCTGCAAGCACGTCGCCAC‑aaac                                                               >  1:399696/1‑209 (MQ=255)                                                             ccataCAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCACGTCGCCCC‑AAACTCGCCCCCGTTGCCGGTTTTTAGCTCCCCAACTCGGAAcacca                    >  1:816003/5‑251 (MQ=255)                                                               ataCAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAc                                                                                         >  1:246106/3‑181 (MQ=255)                                                                tacaaaccaatccgtcggCTTAATAACCGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGt                 <  1:307964/238‑1 (MQ=255)                                                                    aaaCAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATTCCGTAAAAATCCCGCAAGCACGGCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACCTcaa              >  1:616932/1‑250 (MQ=255)                                                                    aaaCAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AAACTCGCCCCCGTTGCCGCTTTTAAACTCCCCAACTCCGAACACCACCTcaa              >  1:415614/1‑250 (MQ=255)                                                                       caatccgtcggCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAat          <  1:863034/245‑1 (MQ=255)                                                                         atccgtcggCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAatat        <  1:477199/247‑1 (MQ=255)                                                                                ggCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCCTGCCGTAAACATCCTCCAAGCACGTCGCCCC‑AATCTCGCCCCCGTTGCCGGTTTTTAGCTCCCCCACTCGGAACACCACGTCAACAATATCGCggg  >  1:128418/1‑249 (MQ=255)                                                                                     |                                                                                                                                                                                                                                                       GCCCGCATCAACTATCAAGTCATAGCCGACAGAAAGTGCAGTCGGTTGCGCGCCCAAGCTCCAGACAAAACAGTCGGTCGGCTTGATAAATGCGCGCTCCGCGTCCGATAGCACAAGCGGGAGTACGTGATATGGCACCTCAAACAAG‑CGCGCGGAATCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAACACCACGTCAACAATATCGCGGT  >  1/1483340‑1483668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑