Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,483,403	G→T	intergenic (‑38775/‑158361)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,483,403	0	G	T	96.5%	71.7 / ‑3.4	29	intergenic (‑38775/‑158361)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (18/10);  minor base A (1/0);  total (19/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GCCCGCATCAACTATCAAGTCATAGCCGACAGAAAGTGCAGTCGGTTGCGCGCCCAAGCTCCAGACAAAACAGTCGGTCGGCTTGATAAATGCGCGCTCCGCGTCCGATAGCACAAGCGGGAGTACGTGATATGGCACCTCAAACAAG‑CGCGCGGAATCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAAC  >  1/1483340‑1483646                                                                |                                                                                                                                                                                                                                                      acccacattaaccagcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCa                                                           <  1:851526/232‑1 (MQ=255)             cagcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCACGTCGcc                                                >  1:885890/8‑250 (MQ=255)               gcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCCTGCCGGTAACATCCTGCAAGCACGGCGCcac‑a                                            >  1:123973/6‑251 (MQ=255)               gcatatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAAATCCTGCCAGCACGTCGCcac‑a                                            >  1:336286/6‑251 (MQ=255)                catatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCATGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCcac                                              <  1:431013/245‑1 (MQ=255)                  tatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAgcgg                                                                                   >  1:87667/3‑210 (MQ=255)                  tatCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAAATCCTGCAAGCACCTCGCCCC‑AAt                                          >  1:493069/3‑250 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAc                                                                   <  1:587443/224‑1 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCCC‑AAt                                          >  1:792839/2‑249 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCACGTCGCCCC‑AAt                                          >  1:330367/2‑249 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCCTAAACATCCTTCCAGCACGTCGCcaccaac                                          >  1:685481/2‑246 (MQ=255)                   atCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCAAACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCAAGCCGTAAACATCCTGCAAGCa                                                       >  1:114049/2‑237 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGagtag                                                                                          >  1:715757/1‑201 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTa                                                                                       <  1:7335/204‑1 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCCTTGCGTGAACATCCTGCAAggccggctccccaaact                                         >  1:429749/1‑234 (MQ=255)                    tCATAGCCCACCGAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAACATCCTGCAAGCACGTCGCCAC‑AAtct                                        >  1:534190/1‑250 (MQ=255)                         gcccaccgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCa                                                       <  1:251048/224‑1 (MQ=255)                             accgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGGATGCCGTAAAAATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCCCGtt                              >  1:616503/4‑251 (MQ=255)                              ccgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTg                             <  1:736308/249‑1 (MQ=255)                              ccgAAAGCGCTGTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAAAAACCTGCAAGCACGTCGCCAC‑AATCTCGCCCCCGTTg                             >  1:224377/3‑251 (MQ=255)                                 aaagcgctgTCGGCTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTg                                                                                               <  1:446435/175‑1 (MQ=255)                                         gTCGGTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGCGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGTCGTAAACATCCTGCAAGAACGGCGACACAAATC‑CGCCCCCGTTTCCGTTTTTaca                  >  1:507157/1‑249 (MQ=255)                                             gTTGCGCGCCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCCAGCACGTCGCCCC‑AATCTCGCCCCCGTTGCCGTTTTTAAGCTc               >  1:524924/1‑250 (MQ=255)                                                   cgcCCAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTgtgt                                                                                                 >  1:750627/1‑163 (MQ=255)                                                     cccAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAg                                                                                                                   <  1:650996/143‑1 (MQ=255)                                                     cccAGGCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AAACTCGCCCCCGTTGCCGGTTTTAAGCTCCCCAAccc       >  1:575099/1‑248 (MQ=255)                                                          gCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAac  <  1:294858/250‑1 (MQ=255)                                                          gCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAac  <  1:270186/250‑1 (MQ=255)                                                          gCTCCATACAAAACAATCCGTCGGCTTAATAAACGCACGCTCGGCATCGGATAGCACCAGCGGTAGCACGTGATACGGCACCTCGAA‑AAGCCGCGCCTGCTCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGGAAAAATCCTGCAAGCACGTCGCCAC‑aaac                                         >  1:399696/1‑209 (MQ=255)                                                                |                                                                                                                                                                                                                                                      GCCCGCATCAACTATCAAGTCATAGCCGACAGAAAGTGCAGTCGGTTGCGCGCCCAAGCTCCAGACAAAACAGTCGGTCGGCTTGATAAATGCGCGCTCCGCGTCCGATAGCACAAGCGGGAGTACGTGATATGGCACCTCAAACAAG‑CGCGCGGAATCAATCGGTTTGGCGGTGTAATCGGGCGGCACGTAAAGCGACTCGCCTTTTTGTGTTGAGTAGTTAGCGGCGGGCATGCCGTAAACATCCTGCAAGCACGTCGCCAC‑AATCTCGCCCTCGTTGCCGTTTTTAAGCTCCCCAACTCGGAAC  >  1/1483340‑1483646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑