Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,481,430	A→G	intergenic (‑36802/‑160334)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,481,430	0	A	G	100.0%	43.5 / NA	21	intergenic (‑36802/‑160334)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/18);  total (3/18)

TAATTCTTTGTGCACCATCAAATGCCTGCCAATTATTGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCAGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCATGGGCATTTGGATAATCTGATTTTCTAATACAATTCCAAAAATCTTTATGATAAAGATCACTAAGTGTTGCGACTTGCTGACCGCTAAATAATAGCCAACTGGCTAAGCAGTTTAAATCCTTATAACCGTCAAATGTTTCGATTACTTTGATTATTTTAGTTTTGAGAAGATGA  >  1/1481188‑1481657                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    tAATTCTTTGTGCACCATCAAATGCCTGCCAATTATTGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTaa                                                                                                                                                                                                                              <  1:527464/250‑1 (MQ=37)  aaTTCTTTGTGCACCATCAAATGCCTGCCAATTATTGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACg                                                                                                                                                                                                                            <  1:85443/251‑1 (MQ=37)  aaTTCTTTGTGCACCATCAAATGCCTGCCAATTATTGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACg                                                                                                                                                                                                                            <  1:749754/251‑1 (MQ=37)  aaTTCTTTGTGCACCATCAAATGCCTGCCAATTATTGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACg                                                                                                                                                                                                                            <  1:406892/251‑1 (MQ=37)                    aaaTGCCTGCCAATTATTGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTgg                                                                                                                                                                                                          <  1:394526/251‑1 (MQ=18)                       tgcctgccAATTATTGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTaa                                                                                                                                                                                                       <  1:870993/251‑1 (MQ=18)                                attattGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGaa                                                                                                                                                                                                                     <  1:826012/228‑1 (MQ=17)                                attattGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                                                                                                                                                                                              <  1:38697/251‑1 (MQ=14)                                attattGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                                                                                                                                                                                              <  1:512813/251‑1 (MQ=14)                                attattGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                                                                                                                                                                                              <  1:542332/251‑1 (MQ=14)                                attattGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                                                                                                                                                                                              <  1:240976/251‑1 (MQ=14)                                attattGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                                                                                                                                                                                              <  1:750506/251‑1 (MQ=14)                                attattGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                                                                                                                                                                                              <  1:860379/251‑1 (MQ=14)                                attattGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                                                                                                                                                                                              <  1:174486/251‑1 (MQ=14)                                              ccTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCa                                                                                                                                                                                <  1:362020/251‑1 (MQ=11)                                               cTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATAc                                                                                                                                                                                              <  1:209066/236‑1 (MQ=11)                                                                                                                                                                                                                 cacaAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCATGGGCATTTGGATAATCTGATTTTCTAATACAATTCCAAAAATCTTTATGATAAAGATCACTAAGTGTTGCGACTTGCTGACCGCTAAATAATAGCCAACTGGCTAAGCAGTTTAAATCCTTATAACCGTCAAATGTTTCGATTACTTTGATTATTTTAGttt             >  1:396270/1‑251 (MQ=255)                                                                                                                                                                                                                 cacaAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCATGGGCATTTGGATAATCTGATTTTCTAATACAATTCCAAAAATCTTTATGATAAAGATCACTAAGTGTTGCGACTTGCTGACCGCTAAATAATAGCCAACTGGCTAAGCAGTTTAAATCCTTATAACCGTCAAATGTTTCGATTACTTTGATTATTTTAGttt             <  1:551408/251‑1 (MQ=255)                                                                                                                                                                                                                   caAATACCGCCCTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCATGGGCATTTGGATAATCTGATTTTCTAATACAATTCCAAAAATCTTTATGATAAAGATCACTAAGTGTTGCGACTTGCTGACCGCTAAATAATAGCCAACTGGCTAAGCAGTTTAAATCCTTATAACCGTCAAATGTTTCGATTACTTTGatta                      >  1:785725/1‑240 (MQ=255)                                                                                                                                                                                                                            cccTTTCGTCATTGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCATGGGCATTTGGATAATCTGATTTTCTAATACAATTCCAAAAATCTTTATGATAAAGATCACTAAGTGTTGCGACTTGCTGACCGCTAAATAATAGCCAACTGGCTAAGCAGTTTAAATCCTTATAACCGTCAAATGTTTCGATTACTTTGATTATTTTAGTTTTGAGAAgatga  <  1:258403/251‑1 (MQ=255)                                                                                                                                                                                                                                        tGATAAAACGCGGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCATGGGCATTTGGATAATCTGATTTTCTAATACAATTCCAAAAATCTTTATGATAAAGATCACTAAGTGTTGCGACTTGCTGACCGCTAAATAATAGCCAACTGGCTAAGCAGTTTAAATCCTTATAACCGTCaaa                                          >  1:547277/1‑199 (MQ=14)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                    TAATTCTTTGTGCACCATCAAATGCCTGCCAATTATTGACTTTATCCTCACACATATACTCAATAAACCGCACGAACTCGCTTTTTGTCGATGAGAAGAAGATATACGGTGGTCGTGTAATGTTAATTAAGCGTAGGAAGTCGATAAGATCGAAATAATGCGCTTGCTTATAGCTCTCTTGTTTCGTACACAAATATGGCGGGTCTAACACAAATACCGCCCTTTCGTCATTGATAAAACGCAGAAGTAACGTGTGGAATGATTCATTGGTAATCTCTATACCATCCAAATAACCATGGGCATTTGGATAATCTGATTTTCTAATACAATTCCAAAAATCTTTATGATAAAGATCACTAAGTGTTGCGACTTGCTGACCGCTAAATAATAGCCAACTGGCTAAGCAGTTTAAATCCTTATAACCGTCAAATGTTTCGATTACTTTGATTATTTTAGTTTTGAGAAGATGA  >  1/1481188‑1481657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑