Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,475,087	C→T	intergenic (‑30459/‑166677)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,475,087	0	C	T	100.0%	196.6 / NA	72	intergenic (‑30459/‑166677)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/35);  total (37/35)

TTATTCGTTTTGCCGGTCTTGTGGGGCAAGATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACCGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGTCAAATACCATTTTGCAACACTTACCGAA  >  1/1474844‑1475331                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     ttATTCGTTTTGCCGGTCTTGTGGGGCAAAATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACttt                                                                                                                                                                                                                                               <  1:386746/251‑1 (MQ=255)    ttCGTTTTGCCGGTCTTGTGGGGCAAAATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATc                                                                                                                                                                                                                                            <  1:384377/251‑1 (MQ=255)      cGTTTTGCCGGTCTTGTGGGGCAAAATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAg                                                                                                                                                                                                                                          <  1:68531/251‑1 (MQ=255)       cgtttGCCGGTCTTGTGGGGCAAAATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAg                                                                                                                                                                                                                                          >  1:721704/3‑250 (MQ=255)         tttGCCGGTCTTGTGGGGCAAAATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAgcgc                                                                                                                                                                                                                                       <  1:50689/251‑1 (MQ=255)                gTCTTGTGGGGCAAAATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGaa                                                                                                                                                                                                                                >  1:647141/1‑251 (MQ=255)                gTCTTGTGGGGCAAAATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGaa                                                                                                                                                                                                                                >  1:416480/1‑251 (MQ=255)                gTCTTGTGGGGCAAAATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGaa                                                                                                                                                                                                                                >  1:808512/1‑251 (MQ=255)                   ttGTGGGGCAAAATCGCCATCCGATTTTTTCCCTCGCCGCCAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATGTGGATGACTGCGCCCGCGCCAGTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTACTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGAt                                                                                                                                                                                                                             <  1:835150/251‑1 (MQ=255)                        gggCAAAATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCcaca                                                                                                                                                                                                                        >  1:776893/1‑251 (MQ=255)                        gggCAAAATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCcac                                                                                                                                                                                                                         <  1:390105/250‑1 (MQ=255)                          gCAAAATCGCCATCCGATTTCTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAg                                                                                                                                                                                                                       <  1:470489/250‑1 (MQ=255)                                   ccATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATtg                                                                                                                                                                                                             <  1:369588/251‑1 (MQ=255)                                      tCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTgg                                                                                                                                                                                                          <  1:199183/251‑1 (MQ=255)                                                       ccGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCcac                                                                                                                                                                                                                         >  1:660721/1‑219 (MQ=255)                                                         gACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATc                                                                                                                                                                                                                                            >  1:17103/1‑198 (MQ=255)                                                                 ggATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGAtt                                                                                                                                                                               >  1:184744/1‑251 (MQ=255)                                                                  gATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGAttt                                                                                                                                                                              >  1:837408/1‑251 (MQ=255)                                                                    tctcAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCg                                                                                                                                                                            >  1:799366/1‑251 (MQ=255)                                                                     ctcAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGAt                                                                                                                                                                                <  1:309028/246‑1 (MQ=255)                                                                           tGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATc                                                                                                                                                                      <  1:749700/250‑1 (MQ=255)                                                                               gTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATa                                                                                                                                                                  >  1:307761/1‑250 (MQ=255)                                                                                 cGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACGCCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATAccc                                                                                                                                                               <  1:531443/251‑1 (MQ=255)                                                                                        cGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCa                                                                                                                                                        <  1:465539/251‑1 (MQ=255)                                                                                          gTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATAcccc                                                                                                                                                              <  1:736985/243‑1 (MQ=255)                                                                                             aaTTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAAt                                                                                                                                                     >  1:394503/1‑249 (MQ=255)                                                                                                       aTTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGt                                                                                                                                                                           >  1:827608/1‑217 (MQ=255)                                                                                                            gATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGgaag                                                                                                                                     <  1:767933/250‑1 (MQ=255)                                                                                                                aCTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATAccc                                                                                                                                                               <  1:475243/220‑1 (MQ=255)                                                                                                                    cgcCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGt                                                                                                                            >  1:486653/1‑251 (MQ=255)                                                                                                                            ccATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGcggtcgg                                                                                                                    >  1:438178/1‑251 (MQ=255)                                                                                                                            ccATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACAGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATAccc                                                                                                                                                               <  1:869568/208‑1 (MQ=255)                                                                                                                              aTTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACACAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGtt                                                                                                                  >  1:441105/1‑251 (MQ=255)                                                                                                                              aTTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGtt                                                                                                                  >  1:648145/1‑251 (MQ=255)                                                                                                                                 cAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATc                                                                                                                                                    <  1:289306/214‑1 (MQ=255)                                                                                                                                 cAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAg                                                                                                                             >  1:237284/1‑237 (MQ=255)                                                                                                                                    cTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCt                                                                                                                                                   >  1:875640/1‑212 (MQ=255)                                                                                                                                            ggAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCcac                                                                                                                                                                                                                         >  1:487805/1‑134 (MQ=255)                                                                                                                                            ggAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAAt                                                                                                                                                                                                <  1:57186/159‑1 (MQ=255)                                                                                                                                                 cacCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGtt                                                                                               >  1:788873/1‑251 (MQ=255)                                                                                                                                                         ggCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATAccc                                                                                                                                                               <  1:364089/179‑1 (MQ=255)                                                                                                                                                         ggCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACtttt                                                                                       >  1:864785/1‑251 (MQ=255)                                                                                                                                                          gCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACttttt                                                                                      >  1:582530/1‑251 (MQ=255)                                                                                                                                                          gCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACttttt                                                                                      >  1:630298/1‑251 (MQ=255)                                                                                                                                                                 ccTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTaaaa                                                                               >  1:795212/1‑251 (MQ=255)                                                                                                                                                                  ttGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATAccc                                                                                                                                                               <  1:425563/169‑1 (MQ=255)                                                                                                                                                                  cTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTaaaa                                                                               >  1:333641/1‑250 (MQ=255)                                                                                                                                                                    gTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATc                                                                                                                                                    <  1:287765/179‑1 (MQ=255)                                                                                                                                                                    gTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTg                                                                                                                           >  1:6932/1‑204 (MQ=255)                                                                                                                                                                       tCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATc                                                                                                                                                                      >  1:532726/1‑158 (MQ=255)                                                                                                                                                                       tCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGtt                                                                         >  1:327617/1‑251 (MQ=255)                                                                                                                                                                            tGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAAcc                                                                    <  1:555917/251‑1 (MQ=255)                                                                                                                                                                                        tGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAacac                                                        <  1:409644/251‑1 (MQ=255)                                                                                                                                                                                          cATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAg                                                                                                            <  1:352306/197‑1 (MQ=255)                                                                                                                                                                                                     cgAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAgg                                           <  1:531845/251‑1 (MQ=255)                                                                                                                                                                                                         gCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAg                                                                                                                                                                                                                               >  1:200550/1‑67 (MQ=255)                                                                                                                                                                                                         gCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTg                                                                                                                           <  1:577447/167‑1 (MQ=255)                                                                                                                                                                                                         gCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGgaaag                                       >  1:404228/1‑251 (MQ=255)                                                                                                                                                                                                         gCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGgaaag                                       >  1:360828/1‑251 (MQ=255)                                                                                                                                                                                                           cTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAAt                                                              >  1:191867/1‑226 (MQ=255)                                                                                                                                                                                                                 ccGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACacaaaac                                                   <  1:593355/231‑1 (MQ=255)                                                                                                                                                                                                                  cGCTTGGCAGCGGAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGt                              <  1:366387/251‑1 (MQ=255)                                                                                                                                                                                                                              gAAAAACACCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGTCAAATACCAttt                  >  1:840087/1‑251 (MQ=255)                                                                                                                                                                                                                                   acacCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGTCAAATACCATTTTGCaa             >  1:659127/1‑251 (MQ=255)                                                                                                                                                                                                                                    cacCATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGTCAAATACCATTTTGCAac            <  1:734191/251‑1 (MQ=255)                                                                                                                                                                                                                                      ccATGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGTCAAATACCATTTTGCAacac          <  1:623125/251‑1 (MQ=255)                                                                                                                                                                                                                                        aTGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGTCAAATACCAt                    <  1:714118/239‑1 (MQ=255)                                                                                                                                                                                                                                         tGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAat                                  <  1:583515/224‑1 (MQ=255)                                                                                                                                                                                                                                         tGTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGTCAAATACCATTTTGCAacac          <  1:700064/248‑1 (MQ=255)                                                                                                                                                                                                                                          gTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAAc                                                                              >  1:521723/1‑179 (MQ=255)                                                                                                                                                                                                                                          gTAAATCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGTCAAATACCATTTTGCAACACTTa       >  1:758043/1‑250 (MQ=255)                                                                                                                                                                                                                                              aTCCACTGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGTCAAATACCATTTTGCAACACTTACCGaa  <  1:535935/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     TTATTCGTTTTGCCGGTCTTGTGGGGCAAGATCGCCATCCGATTTTTTCCCTCGCCGACAAACAGGATCTCAAGTGCGGTCGTTCGCCGGTGAATTTAGTGCATTTGGATGACTGCGCCCGCGCCATTCAACTATTGCTGGAAACACCCGGTGGCTATCGCCTGTATCATTTGGCAGCGCCCGTGCATCCGCCCCGCGAAGCCTATTACCGCTTGGCAGCGGAAAAACACCATGTAAATCCACCGCACTTTATCAGCGCACCGGAAGATCCACAGCGCATTATTGTGGCGAACAAAATTTGTCGTGAACTGGATTTCGTTTATCAATACCCCGATCCAAATCTGATGTTTTCGGAAGAATAAAAGTGCGGTCGGTTTTTAAGTTATTTTTTAGTTGCACTTTTTATTAAAACTCGTTTAACCCAAAATAAACACAAAACAAATAAGGAAAGAAAATATGTCAAATACCATTTTGCAACACTTACCGAA  >  1/1474844‑1475331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑