Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,466,521	A→G	intergenic (‑21893/‑175243)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,466,521	0	A	G	100.0%	24.8 / NA	11	intergenic (‑21893/‑175243)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/4);  total (7/4)

TCAGGATTTGGGTTATCAACTTCTTTAAACGCAGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGACCACCGCCAAAACACATGGACGC  >  1/1466489‑1466759                                 |                                                                                                                                                                                                                                               gctgtcaagccctgccatcgcccgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGacca                      >  1:238173/26‑251 (MQ=255)  ctgtcaagccctgccatcgcccgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGaccac                     <  1:359762/227‑1 (MQ=255)  ctgtcaagccctgccatcgcccgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGacca                      >  1:873571/25‑250 (MQ=255)   tgtcaagccctgccatcgcccgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGaccac                     >  1:512109/24‑250 (MQ=255)    gtcaagccctgccatcgcccgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGACCACCg                   >  1:100906/23‑251 (MQ=255)    gtcaagccctgccatcgcccgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGACCACCg                   >  1:697399/23‑251 (MQ=255)         gccctgccatcgcccgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGACCACCGCCaaa              >  1:746052/18‑251 (MQ=255)              gccatcgcccgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGc                                                                                                >  1:627508/13‑164 (MQ=255)                     cccgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGACCACCGCCAAAACACATGGACGc  <  1:521913/246‑1 (MQ=255)                     cccgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGACCACCGCCAAAACACATGGACGc  <  1:772801/246‑1 (MQ=255)                       cgatAAACGCGGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGc                                                                                                <  1:866076/152‑1 (MQ=255)                                 |                                                                                                                                                                                                                                               TCAGGATTTGGGTTATCAACTTCTTTAAACGCAGGGAAAATCCAAATCGACTCCTGCTTCAATCAGTTGGGTACTGACCACCCATAAAGGCTTTTTCAGACGACCTTCGCGATACAGTTCCAAATAACGGCGAACCAGTGCAATCACTTCGCTACGGTGCGTAGCACACATATTGGCAGAGAGATGAAGTTTGATGCCGTTTGAAGGCAGCGCGGCAAAAAGCCTACGGGCATGTTTGCGGGTATTGACCACCGCCAAAACACATGGACGC  >  1/1466489‑1466759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑