Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,465,402	A→G	intergenic (‑20774/‑176362)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,465,402	0	A	G	100.0%	195.0 / NA	69	intergenic (‑20774/‑176362)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/31);  total (38/31)

TTTTAATTTCAATTGTTTTAATTTTCATATTTTTTCCTTATTTCAAGATACTCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACAGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAATCCAGCACGCCGCTGATGCCCAGTTTCTCCGCCGAAACGTGAACGGT  >  1/1465157‑1465632                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       ttttAATTTCAATTGTTTTAATTTTCATATTTTTTCCTTATTTCAAGATACTCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGac                                                                                                                                                                                                                                   <  1:674077/251‑1 (MQ=255) ttttAATTTCAATTGTTTTAATTTTCATATTTTTTCCTTATTTCAAGATACTCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGac                                                                                                                                                                                                                                   <  1:620638/251‑1 (MQ=255)           aaTTGTTTTAATTTTCATATTTTTTCCTTATTTCAAGATACTCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCgg                                                                                                                                                                                                                         <  1:141706/251‑1 (MQ=255)                     aTTTTCATATTTTTTCCTTATTTCAAGATACTCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCatat                                                                                                                                                                                                               <  1:312457/251‑1 (MQ=255)                                        aTTTCAAGATACTCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAg                                                                                                                                                                                            >  1:63530/1‑251 (MQ=255)                                         tttCAAGATACTCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAgg                                                                                                                                                                                           >  1:461679/1‑251 (MQ=255)                                            cAAGATACTCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGCCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGGTTACCTATACCACAGCGCGCCCTCAAGGACg                                                                                                                                                                                        >  1:449624/1‑251 (MQ=255)                                                 tACTCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTg                                                                                                                                                                                   >  1:151087/1‑251 (MQ=255)                                                    tCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGccc                                                                                                                                                                                <  1:235261/251‑1 (MQ=255)                                                     cAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACcac                                                                                                                                                                                                          <  1:820839/224‑1 (MQ=255)                                                     cAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACgg                                                                                                                                                                                       >  1:41658/1‑243 (MQ=255)                                                         taatTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCa                                                                                                                                                                           >  1:364358/1‑251 (MQ=255)                                                              cATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATAc                                                                                                                                                                                                             <  1:731540/212‑1 (MQ=255)                                                              cATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACcac                                                                                                                                                                                                          <  1:683784/215‑1 (MQ=255)                                                              cATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTtc                                                                                                                                                                       >  1:396905/1‑250 (MQ=255)                                                                tCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACg                                                                                                                                                                                                                                >  1:361412/1‑191 (MQ=255)                                                                tCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATAcc                                                                                                                                                                                                            >  1:530999/1‑211 (MQ=255)                                                                tCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACcac                                                                                                                                                                                                          >  1:738982/1‑213 (MQ=255)                                                                tCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACcac                                                                                                                                                                                                          <  1:236342/213‑1 (MQ=255)                                                                tCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTtcct                                                                                                                                                                     >  1:602887/1‑250 (MQ=255)                                                                tCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTtcct                                                                                                                                                                     >  1:498092/1‑250 (MQ=255)                                                                tCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTtcct                                                                                                                                                                     >  1:667055/1‑250 (MQ=255)                                                                tCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGGTTGCCCCGGCATTtcct                                                                                                                                                                     >  1:689000/1‑250 (MQ=255)                                                                  tACATCCCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAg                                                                                                                                                                                                        <  1:20659/213‑1 (MQ=255)                                                                   aCATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCaa                                                                                                                                                                 >  1:640187/1‑251 (MQ=255)                                                                   aCATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCaa                                                                                                                                                                 >  1:421725/1‑251 (MQ=255)                                                                   aCATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGAACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATAcc                                                                                                                                                                                                            <  1:54547/208‑1 (MQ=255)                                                                          ccgccgACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCat                                                                                                                                                                                                                 >  1:573883/1‑196 (MQ=255)                                                                                aCCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTgg                                                                                                                                                    >  1:887772/1‑251 (MQ=255)                                                                                 ccTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTggg                                                                                                                                                   <  1:583611/251‑1 (MQ=255)                                                                                 ccTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTggg                                                                                                                                                   >  1:526505/1‑251 (MQ=255)                                                                                    aTCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGc                                                                                                                                                          <  1:417361/241‑1 (MQ=255)                                                                                           ttCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTCGCAGCGTTTGCCGTAGTCGGGAGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGGTTGCATATACCACAGCGCGCCCCCAAAGACGGCTTGCCCCGTCATTTCCTTCAAACACAGACCTTGGGCGCaaaa                                                                                                                                            >  1:201320/1‑247 (MQ=255)                                                                                                    aaTGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTc                                                                                                                                <  1:777963/251‑1 (MQ=255)                                                                                                              ggCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCa                                                                                                                      >  1:895729/1‑251 (MQ=255)                                                                                                                  aaTCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGtt                                                                                                                  <  1:889584/251‑1 (MQ=255)                                                                                                                    tctcCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTg                                                                                                                >  1:803167/1‑251 (MQ=255)                                                                                                                     ctcCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTgg                                                                                                               >  1:332760/1‑251 (MQ=255)                                                                                                                     ctcCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGGTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGgcg                                                                                                                                                 >  1:364880/1‑217 (MQ=255)                                                                                                                       ccaccaGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGc                                                                                                             <  1:286762/251‑1 (MQ=255)                                                                                                                               gAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGc                                                                                                             <  1:352174/243‑1 (MQ=255)                                                                                                                                     gCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGAt                                                                                                                                      >  1:213692/1‑212 (MQ=255)                                                                                                                                     gCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTtata                                                                                               >  1:703134/1‑251 (MQ=255)                                                                                                                                                   gCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCa                                                                                 >  1:157692/1‑251 (MQ=255)                                                                                                                                                   gCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGGGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTatat                                                                                              <  1:435912/238‑1 (MQ=255)                                                                                                                                                          tCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGAcc                                                                          <  1:241505/251‑1 (MQ=255)                                                                                                                                                             ggTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTTCACGGGTTTCAgacgac                                                                           >  1:276408/1‑247 (MQ=255)                                                                                                                                                                ggCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTggg                                                                                                              <  1:260100/209‑1 (MQ=255)                                                                                                                                                                    gggTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGcc                                                                                                                                                                                 <  1:294429/138‑1 (MQ=255)                                                                                                                                                                                 ttCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTccacca                                                   >  1:263647/1‑251 (MQ=255)                                                                                                                                                                                   ccAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAga                                                                               >  1:369784/1‑221 (MQ=255)                                                                                                                                                                                    cAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGc                                                                                                             <  1:201032/190‑1 (MQ=255)                                                                                                                                                                                      gTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAATc                                               >  1:776089/1‑250 (MQ=255)                                                                                                                                                                                       tAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAAt                                                <  1:297995/248‑1 (MQ=255)                                                                                                                                                                                       tAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAAt                                                <  1:177959/248‑1 (MQ=255)                                                                                                                                                                                       tAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAAt                                                <  1:760182/248‑1 (MQ=255)                                                                                                                                                                                          tCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAATCCAGCa                                          >  1:764809/1‑251 (MQ=255)                                                                                                                                                                                             acggacggCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTc                                                                                            <  1:138802/198‑1 (MQ=255)                                                                                                                                                                                                        cgATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAATCCAGCACGCCGCTGATGccc                            <  1:595092/251‑1 (MQ=255)                                                                                                                                                                                                         gATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAATCCAGCACGCCGCTGATGCCCa                           >  1:202842/1‑251 (MQ=255)                                                                                                                                                                                                             gtggCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTggg                                                                                                                                                   >  1:757792/1‑127 (MQ=255)                                                                                                                                                                                                             gtggCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTccac                                                     >  1:207351/1‑221 (MQ=255)                                                                                                                                                                                                             gtggCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAATCCAGCACGCCGCTGATGCCCAGtt                        <  1:486474/250‑1 (MQ=255)                                                                                                                                                                                                                       tgcgggtgcggAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAATCCAGCACGCCGCTGATGCCCAGTTTCTCCGCCGa              <  1:98201/250‑1 (MQ=255)                                                                                                                                                                                                                         cgggtgcggAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAAt                                                <  1:347506/214‑1 (MQ=255)                                                                                                                                                                                                                          gggtgcggAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTTCATATAcc                                                                                                                                                                                                            >  1:513439/1‑57 (MQ=255)                                                                                                                                                                                                                           ggtgcggAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAacac                                                                                                                                                             >  1:408909/1‑103 (MQ=255)                                                                                                                                                                                                                           ggtgcggAGGTCGTCTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAATCCAGCACGCCGCTGATGCCCAGTTTCTCCGCCGAAACGt         >  1:641670/1‑251 (MQ=255)                                                                                                                                                                                                                                    gtcgtcTGAAAAGACGACGGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAATCCAGCACGCCGCTGATGCCCAGTTTCTCCGCCGAAACGTGAACGGt  <  1:631723/249‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       TTTTAATTTCAATTGTTTTAATTTTCATATTTTTTCCTTATTTCAAGATACTCAAATAATTCATCTACATACCCCGCCGACCTATCCCGTTTCCCCAACAATGCCGGCTGGCAAATCTCCACCAGCGAGCAGGCCTTGCAGCGTTTGCCGTAGTCGGGTGGCGGGGTTTGTCCGCTTTCCAGTAATCCACGGACGGCGGCGATGGTGGCTAGCGTGCGGGTGCGGAGGTCGTCTGAAAAGACGACAGGGACACGGTGGCGGGTTTGCATATACCACAGCGCGCCCTCAAGGACGGTTTGCCCCGTCATTTCCTCCAAACACAGCCCTTGGGCGCAAAGCTGGATTTCGTCCATAGGCTCAAGTTTGGGCTTGCCGCGTTTATATTCCACGGGTTTCAGACGACCTGTTTTCGCCTCGACCTCCACCAAATCCAGCACGCCGCTGATGCCCAGTTTCTCCGCCGAAACGTGAACGGT  >  1/1465157‑1465632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑