| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,463,507 | C→T | intergenic (‑18879/‑178257) | unknown ← / → unknown | tRNA‑Met‑CAT/tRNA‑Gly‑GCC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,463,507 | 0 | C | T | 97.8% | 116.2 / NA | 46 | intergenic (‑18879/‑178257) | unknown/unknown | tRNA‑Met‑CAT/tRNA‑Gly‑GCC |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (20/25); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGTTGCGCCACTTTCTTCTTTTCCTGCTCCACTACCAACGTCTCCCGCTCCTTATGCAGATAACTGCCCTGAGT‑GGTGATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGCCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAGTGATATATTTTATCTTAAATTTATTTAATAATTTCAAGTACAAAGGTATATTTCCTTTTG > 1/1463269‑1463746 | cAGTTGCGCCACTTTCTTCTTTTCCTGCTCCACTACCAACGTCTCCCGCTCCTTATGCAGATAACTGCCCTGAGT‑GGTGATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCt < 1:869434/250‑1 (MQ=255) gcgcCACTTTCTTCTTTTCCTGCTCCACTACCAACGTCTCCCGCTCCTTATGCAGATAACTGCCCTGAGT‑GGTGATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTcc > 1:827268/1‑248 (MQ=255) gCTCCACTACCAACGTCTCCCGCTCCTTATGCAGATAACTGCCCTGAGT‑GGTGATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCaa > 1:58605/1‑251 (MQ=255) aCCAACGTCTCCCGCTCCTTATGCAGATAACTGCCCTGAGTGGGTGATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGaaaa > 1:27033/1‑213 (MQ=255) ctcCCGCTCCTTATGCAGATAACTGCCCTGAGT‑GGTGATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGaaaa > 1:413455/1‑204 (MQ=255) tCCTTATGCAGATAACTGCCCTGAGT‑GGTGATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAg < 1:252756/198‑1 (MQ=255) aTAACTGCCCTGAGT‑GGTGATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAg < 1:855876/250‑1 (MQ=255) gt‑ggtgATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAAcc > 1:205227/1‑202 (MQ=255) gtgATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATGATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACtctt > 1:492038/1‑249 (MQ=255) gtgATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAg > 1:126565/1‑234 (MQ=255) gtgATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAg < 1:864164/234‑1 (MQ=255) gtgATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACtctt > 1:178285/1‑249 (MQ=255) gATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAg < 1:662375/232‑1 (MQ=255) gtTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTtat > 1:308631/1‑209 (MQ=255) tGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAAtt < 1:470947/251‑1 (MQ=255) gTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAActc > 1:738560/1‑225 (MQ=255) tCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCa < 1:228817/251‑1 (MQ=255) cAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCa > 1:255613/1‑232 (MQ=255) ttATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAg < 1:92203/161‑1 (MQ=255) ttATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAg < 1:581275/161‑1 (MQ=255) tGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCatatat < 1:78526/251‑1 (MQ=255) gATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACcac > 1:248519/1‑99 (MQ=255) tatTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAGTGa < 1:158416/251‑1 (MQ=255) ttCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAGTGatatat < 1:316246/251‑1 (MQ=255) cTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGAt > 1:839557/1‑214 (MQ=255) gTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAAc < 1:368965/69‑1 (MQ=255) gTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAg < 1:246233/105‑1 (MQ=255) gTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTgg < 1:228990/159‑1 (MQ=255) gTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCatatat < 1:567158/211‑1 (MQ=255) gTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAg > 1:90207/1‑213 (MQ=255) gTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAg < 1:205532/213‑1 (MQ=255) gTTTAAAACTATCAGATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAg > 1:696056/1‑213 (MQ=255) gATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAg > 1:699601/1‑91 (MQ=255) gATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAg < 1:772040/91‑1 (MQ=255) gATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAActc < 1:51010/104‑1 (MQ=255) gATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATc < 1:528477/158‑1 (MQ=255) gATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCatat > 1:111277/1‑195 (MQ=255) gATTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAg > 1:543547/1‑199 (MQ=255) aTTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAActc < 1:663870/103‑1 (MQ=255) aTTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATag > 1:198419/1‑114 (MQ=255) aTTTTATATATTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAGTGatatat < 1:690860/206‑1 (MQ=255) ttttATATATTGCAAGGCCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGaa > 1:43821/1‑91 (MQ=255) atatatTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATag < 1:877469/109‑1 (MQ=255) atatatTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAg < 1:622177/193‑1 (MQ=255) atatTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAg < 1:701114/83‑1 (MQ=255) atatTGCAAGGTCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAGTGATATATTTTATCTTAAATTTATTTAATAATTTCAAGTACAAAGGTATATTTCCTTTTg > 1:343521/1‑251 (MQ=255) | CAGTTGCGCCACTTTCTTCTTTTCCTGCTCCACTACCAACGTCTCCCGCTCCTTATGCAGATAACTGCCCTGAGT‑GGTGATATAGAGCGTGTTTTGCAGTTTCCGCATGGGTGGCTCCTAAAATTTTTCTTACTTCAATTAACTAGCAATTATAATTTTGATTATTGATATATTTTTCTGATTTTTTCTTCAAAACTTGGGGATAGTTTAAAACTATCAGATTTTATATATTGCAAGGCCTGAAAAGGCTTCCCTGATTTACCACATTCATAACCAAGCTCTTCTTCAATGTCATTTATAAGCATAATAGAATGACCTAACTCTTCTTCAATAGAAGATAAAATTTTATTAGTCAGTTTAGTGGCAACAGCTTTATCATTTTCAGATTTTCTATCTTGATGATCTTGATCATATATAGTGATATATTTTATCTTAAATTTATTTAATAATTTCAAGTACAAAGGTATATTTCCTTTTG > 1/1463269‑1463746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |