Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,462,679	C→T	intergenic (‑18051/‑179085)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,462,679	0	C	T	100.0%	214.4 / NA	80	intergenic (‑18051/‑179085)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/44);  total (36/44)

ATATAGGGCAATAAACCGATTTCCACCTCTTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGCCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACGTGCCGCCGCATCGCCTTCGATACCGCGAACCTCATCAAGATTTT  >  1/1462433‑1462922                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    atatAGGGCAATAAACCGATTTCCACCTCTTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTgg                                                                                                                                                                                                                                                 >  1:598581/1‑251 (MQ=255) atatAGGGCAATAAACCGATTTCCACCTCTTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTgg                                                                                                                                                                                                                                                 >  1:877049/1‑251 (MQ=255) atatAGGGCAATAAACCGATTTCCACCTCTTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTgg                                                                                                                                                                                                                                                 >  1:439042/1‑251 (MQ=255) atatAGGGCAATAAACCGATTTCCACCTCTTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTgg                                                                                                                                                                                                                                                 >  1:18556/1‑251 (MQ=255)   ataGGGCAATAAACCGATTTCCACCTCTTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTggtc                                                                                                                                                                                                                                               <  1:776576/251‑1 (MQ=255)        gCAATAAACCGATTTCCACCTCTTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGgtcggtcg                                                                                                                                                                                                                                          >  1:676622/1‑251 (MQ=255)         cAATAAACCGATTTCCACCTCTTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGtcggtcgg                                                                                                                                                                                                                                         <  1:83339/251‑1 (MQ=255)                 cGATTTCCACCTCTTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGa                                                                                                                                                                                                                                 <  1:570092/251‑1 (MQ=255)                            tctTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGtcggtcgg                                                                                                                                                                                                                                         >  1:384506/1‑232 (MQ=255)                            tctTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGAc                                                                                                                                                                                                                         >  1:378949/1‑248 (MQ=255)                                   ccAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTGCAGATTTTAAGGTTACCGCGCCCCCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATGCGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATcc                                                                                                                                                                                                               <  1:587285/251‑1 (MQ=255)                                        aaCGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGAcc                                                                                                                                                                                                          <  1:567464/251‑1 (MQ=255)                                                    aTTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAg                                                                                                                                                                                              >  1:85109/1‑251 (MQ=255)                                                                   ttCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATc                                                                                                                                                                               <  1:516730/251‑1 (MQ=255)                                                                                      acaacaATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACtatat                                                                                                                                                            <  1:400174/251‑1 (MQ=255)                                                                                         acaaTTTTCTGGCTTCAGATTTTAAGGTTCCCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaag                                                                                                                                                         <  1:23609/251‑1 (MQ=255)                                                                                         acaaTTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaag                                                                                                                                                         <  1:458191/251‑1 (MQ=255)                                                                                         acaaTTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTAGATaag                                                                                                                                                         <  1:362980/251‑1 (MQ=255)                                                                                           aaTTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaagaa                                                                                                                                                       <  1:647855/251‑1 (MQ=255)                                                                                           aaTTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaagaa                                                                                                                                                       <  1:661097/251‑1 (MQ=255)                                                                                           aaTTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaagaa                                                                                                                                                       <  1:483228/251‑1 (MQ=255)                                                                                            aTTTTCTGGCTTCAGATTTTAAGGTTCCCGCACCCCCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGaaa                                                                                                                                                                                                                               <  1:676285/178‑1 (MQ=255)                                                                                             ttttCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaagaaag                                                                                                                                                     <  1:561230/251‑1 (MQ=255)                                                                                             ttttCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaagaaag                                                                                                                                                     <  1:459353/251‑1 (MQ=255)                                                                                                    gCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACtatat                                                                                                                                                            <  1:834831/237‑1 (MQ=255)                                                                                                           aTTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCt                                                                                                                                                                              >  1:63257/1‑212 (MQ=255)                                                                                                           aTTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaagaa                                                                                                                                                       >  1:409765/1‑235 (MQ=255)                                                                                                           aTTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAAGATCCTGCGCTAGGCTGTCCCGGTCTGGTCGGTCGGCGTACAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAGCACTATATAAGAAAGAAAGCAAAGCAt                                                                                                                                         <  1:489343/249‑1 (MQ=255)                                                                                                               tAAGGTTACCGCCCCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCacac                                                                                                                                   <  1:748691/251‑1 (MQ=255)                                                                                                               tAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCacac                                                                                                                                   <  1:439618/251‑1 (MQ=255)                                                                                                                 aGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCg                                                                                                                                 >  1:868524/1‑251 (MQ=255)                                                                                                                   gTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaag                                                                                                                                                         >  1:202763/1‑225 (MQ=255)                                                                                                                    ttACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGtc                                                                                                                               <  1:84268/250‑1 (MQ=255)                                                                                                                    ttACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGtc                                                                                                                               >  1:407972/1‑250 (MQ=255)                                                                                                                       ccGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaag                                                                                                                                                         >  1:191506/1‑221 (MQ=255)                                                                                                                         cccccaccCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTggg                                                                                                                         <  1:743198/248‑1 (MQ=255)                                                                                                                         ccaccacCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTggg                                                                                                                         <  1:714678/250‑1 (MQ=255)                                                                                                                                      cATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaagaa                                                                                                                                                       <  1:186397/208‑1 (MQ=255)                                                                                                                                      cATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaagaa                                                                                                                                                       >  1:405149/1‑208 (MQ=255)                                                                                                                                                     ccgGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGa                                                                                             <  1:83663/251‑1 (MQ=255)                                                                                                                                                       ggTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTggg                                                                                                                         <  1:281040/221‑1 (MQ=255)                                                                                                                                                         tttAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAAcc                                                                                         <  1:631487/251‑1 (MQ=255)                                                                                                                                                              tCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTa                                                                                    >  1:806892/1‑251 (MQ=255)                                                                                                                                                                      cGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaag                                                                                                                                                         >  1:160313/1‑174 (MQ=255)                                                                                                                                                                      cGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACg                                                                            >  1:417955/1‑251 (MQ=255)                                                                                                                                                                      cGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACg                                                                            <  1:216254/251‑1 (MQ=255)                                                                                                                                                                          tAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGAcc                                                                                                                                                                                                          >  1:177868/1‑121 (MQ=255)                                                                                                                                                                             tCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTTCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGc                                                                     <  1:519411/251‑1 (MQ=255)                                                                                                                                                                             tCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGc                                                                     <  1:475299/251‑1 (MQ=255)                                                                                                                                                                               aTACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaagaa                                                                                                                                                       <  1:391289/167‑1 (MQ=255)                                                                                                                                                                               aTACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAg                                                                   >  1:148010/1‑251 (MQ=255)                                                                                                                                                                                tACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGa                                                                  >  1:117054/1‑251 (MQ=255)                                                                                                                                                                                tACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGa                                                                  >  1:523921/1‑251 (MQ=255)                                                                                                                                                                                tACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGa                                                                  >  1:148368/1‑251 (MQ=255)                                                                                                                                                                                 aCTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCacac                                                                                                                                   <  1:877460/185‑1 (MQ=255)                                                                                                                                                                                 aCTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTggg                                                                                                                         <  1:308430/195‑1 (MQ=255)                                                                                                                                                                                 aCTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGAttt                                                                                                            <  1:193305/208‑1 (MQ=255)                                                                                                                                                                                 aCTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGAt                                                                 >  1:729912/1‑251 (MQ=255)                                                                                                                                                                                       cacCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATa                                                           >  1:724169/1‑251 (MQ=255)                                                                                                                                                                                         ccATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATaagaa                                                                                                                                                       <  1:648314/157‑1 (MQ=255)                                                                                                                                                                                         ccATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTggg                                                                                                                         <  1:248440/187‑1 (MQ=255)                                                                                                                                                                                         ccATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATa                                                           <  1:546345/249‑1 (MQ=255)                                                                                                                                                                                         ccATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATa                                                           <  1:264859/249‑1 (MQ=255)                                                                                                                                                                                         ccATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACg                                                         >  1:123802/1‑251 (MQ=255)                                                                                                                                                                                               gATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTAt                                                                                   <  1:572516/219‑1 (MQ=255)                                                                                                                                                                                                   aaCCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGAcc                                                                                                                                                                                                          <  1:133611/96‑1 (MQ=255)                                                                                                                                                                                                    aCCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACg                                              >  1:443036/1‑251 (MQ=255)                                                                                                                                                                                                        accaAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACGTgc                                           >  1:163072/1‑250 (MQ=255)                                                                                                                                                                                                                                   ccTGCGCTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACGTGCCGCCGCATCGCCTTCGATACCGCGAAcc               <  1:820420/251‑1 (MQ=255)                                                                                                                                                                                                                                      gcgcTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGaaagcaaag                                                                                                                                            <  1:663856/123‑1 (MQ=255)                                                                                                                                                                                                                                      gcgcTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTAt                                                                                   >  1:312305/1‑180 (MQ=255)                                                                                                                                                                                                                                      gcgcTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACg                                              <  1:510604/217‑1 (MQ=255)                                                                                                                                                                                                                                      gcgcTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACGTGCCGCCGCATCGCCTTCGATACCGCGAACCtca            >  1:259532/1‑251 (MQ=255)                                                                                                                                                                                                                                      gcgcTAGGCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACGTGCCGCCGCATCGCCTTCGATACCGCGAACCtca            >  1:549278/1‑251 (MQ=255)                                                                                                                                                                                                                                            ggCTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAacac                                                                                                                                                                 >  1:626331/1‑96 (MQ=255)                                                                                                                                                                                                                                              cTGTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGGCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACGTGCCGCCGCATCGCCTTCGATACCGCGAACCTCATCAAGAt     <  1:372920/250‑1 (MQ=255)                                                                                                                                                                                                                                                gTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATa                                                           >  1:316819/1‑194 (MQ=255)                                                                                                                                                                                                                                                gTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACGTGCCGCCGCATCGCCTTCGATACCGCGAACCTCATCAAGAtttt  >  1:215544/1‑251 (MQ=255)                                                                                                                                                                                                                                                gTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACGTGCCGCCGCATCGCCTTCGATACCGCGAACCTCATCAAGAtttt  >  1:48107/1‑251 (MQ=255)                                                                                                                                                                                                                                                gTCCCTGTCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACGTGCCGCCGCATCGCCTTCGATACCGCGAACCTCATCAAGAtttt  >  1:112491/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ATATAGGGCAATAAACCGATTTCCACCTCTTCGCCCAAAAACGGATGAACGATTTTCTCCTGTTTTTTCGCCTGCAACGCCTGAAACAACAATTTTCTGGCTTCAGATTTTAAGGTTACCGCACCACCCGCCTCATTAATAAAATCCGCCGGTTTAATCTGCAAGCGATTAATCATACTTAACACCATACGATCAACCCACCAAGCGCGAAATTCTTCCAAAATATCCTGCGCTAGGCTGTCCCTGCCTGGTCGGTCGGCGTGCAGAAAACCGACCTGAGGATCCAGACCTACGCCTTGCAGCGCGCCGCTAATATCTTTCCCCAAAACACTATATAAGAAAGAAAGCAAAGCATTCACACCGTCTCTGGGCGGGCGACGATTTCGACCGCCAAAATGAAACCCGGTATTCTCACGAATAAGCAGATTAAATACGCCGAAATAACGTGCCGCCGCATCGCCTTCGATACCGCGAACCTCATCAAGATTTT  >  1/1462433‑1462922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑