Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,450,954	A→G	intergenic (‑6326/‑190810)	unknown ← / → unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,450,954	0	A	G	100.0%	232.9 / NA	81	intergenic (‑6326/‑190810)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gly‑GCC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (47/34);  total (47/34)

ACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGC‑AAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTAGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCGTTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAGTTATTTTGTTCCTTGCCGAAAAGG  >  1/1450711‑1451195                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   aCTTGACGAAAAAACATGACTGAGATCGCATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGa                                                                                                                                                                                                                                              <  1:384318/251‑1 (MQ=255)                             cATTTTAAGCAAAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGt                                                                                                                                                                                                                  >  1:589763/1‑251 (MQ=255)                                                                    tCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCtt                                                                                                                                                                           >  1:126254/1‑251 (MQ=255)                                                                         aTCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GAt                                                                                                                                                                     >  1:151678/1‑251 (MQ=255)                                                                             aTTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGt                                                                                                                                                                 >  1:48543/1‑251 (MQ=255)                                                                             aTTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGt                                                                                                                                                                 >  1:83544/1‑251 (MQ=255)                                                                             aTTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGt                                                                                                                                                                 >  1:626058/1‑251 (MQ=255)                                                                             aTTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGt                                                                                                                                                                 >  1:239622/1‑251 (MQ=255)                                                                                 tATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTc                                                                                                                                                                                                                     >  1:361216/1‑196 (MQ=255)                                                                                    gTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTc                                                                                                                                                                         >  1:78547/1‑237 (MQ=255)                                                                                      atatTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAAt                                                                                                                                                                                                                                           <  1:833277/169‑1 (MQ=255)                                                                                      atatTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTc                                                                                                                                                                                                                     >  1:280734/1‑191 (MQ=255)                                                                                      atatTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGtt                                                                                                                                                                                                                 <  1:374730/195‑1 (MQ=255)                                                                                      atatTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCAttt                                                                                                                                                                                        >  1:731054/1‑220 (MQ=255)                                                                                      atatTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCg                                                                                                                                                         >  1:454297/1‑250 (MQ=255)                                                                                      atatTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTACGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCg                                                                                                                                                         >  1:342223/1‑250 (MQ=255)                                                                                           ataCGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGc                                                                                                                                                                                                                             <  1:428813/178‑1 (MQ=255)                                                                                              cGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GAtt                                                                                                                                                                    >  1:654597/1‑231 (MQ=255)                                                                                                 tAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAaat                                                                                                                                              <  1:560033/250‑1 (MQ=255)                                                                                                 tAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAaat                                                                                                                                              <  1:702667/250‑1 (MQ=255)                                                                                                    gATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCGTCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTg                                                                                                                                                                                      >  1:447323/1‑208 (MQ=255)                                                                                                       cccGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATcc                                                                                                                                                                                                                         <  1:662887/170‑1 (MQ=255)                                                                                                       cccGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGt                                                                                                                                       >  1:366177/1‑251 (MQ=255)                                                                                                                 ttCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATGTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTg                                                                                                                             <  1:446356/251‑1 (MQ=255)                                                                                                                           gCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAAtt                                                                                                                   >  1:483578/1‑251 (MQ=255)                                                                                                                            cGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTg                                                                                                                  <  1:619148/251‑1 (MQ=255)                                                                                                                            cGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTg                                                                                                                  <  1:863232/251‑1 (MQ=255)                                                                                                                                gttCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGc                                                                                                                                                                                                  <  1:298581/168‑1 (MQ=255)                                                                                                                                gttCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACa                                                                                                              >  1:67973/1‑251 (MQ=255)                                                                                                                                gttCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACa                                                                                                              >  1:680615/1‑251 (MQ=255)                                                                                                                                   cATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGTCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTaa                                                                                                                                                               <  1:395839/199‑1 (MQ=255)                                                                                                                                    aTTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATaaa                                                                                                          >  1:461991/1‑251 (MQ=255)                                                                                                                                    aTTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATaaa                                                                                                          >  1:159509/1‑251 (MQ=255)                                                                                                                                    aTTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATaaa                                                                                                          >  1:807536/1‑251 (MQ=255)                                                                                                                                     ttACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGCTGCCATTAAATTGAACATAAAt                                                                                                         >  1:513425/1‑251 (MQ=255)                                                                                                                                           aCTCTACACCTTTTTGTGCAGTAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTaa                                                                                                                                                               <  1:443352/191‑1 (MQ=255)                                                                                                                                            ctctACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAAt                                                                                                         <  1:477639/244‑1 (MQ=255)                                                                                                                                              ctACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCAttt                                                                                                                                                                                        <  1:196485/164‑1 (MQ=255)                                                                                                                                                acacCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCttt                                                                                              >  1:667680/1‑251 (MQ=255)                                                                                                                                                  acCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTg                                                                                            >  1:302819/1‑251 (MQ=255)                                                                                                                                                    cTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCt                                                                                          <  1:102070/251‑1 (MQ=255)                                                                                                                                                     tttttGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGc                                                                                                                                                                                                                             <  1:199597/120‑1 (MQ=255)                                                                                                                                                     tttttGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTa                                                                                         >  1:21199/1‑251 (MQ=255)                                                                                                                                                                cagcTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAAt                                                                                 <  1:494231/248‑1 (MQ=255)                                                                                                                                                                         aagaaTAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTCGGATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATc                                                                       <  1:191920/250‑1 (MQ=255)                                                                                                                                                                         aagaaTAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATcc                                                                      <  1:467712/250‑1 (MQ=255)                                                                                                                                                                         aagaaTAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATcc                                                                      <  1:167304/250‑1 (MQ=255)                                                                                                                                                                         aagaaTAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATcc                                                                      <  1:136218/250‑1 (MQ=255)                                                                                                                                                                         aagaaTAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCt                                                                     <  1:829206/251‑1 (MQ=255)                                                                                                                                                                           gaaTAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCa                                                                                                                                                                                                                            >  1:376435/1‑99 (MQ=255)                                                                                                                                                                           gaaTAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCg                                                                                                                                    <  1:315391/186‑1 (MQ=255)                                                                                                                                                                           gaaTAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTtg                                                                   >  1:37159/1‑251 (MQ=255)                                                                                                                                                                           gaaTAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTtg                                                                   >  1:506322/1‑251 (MQ=255)                                                                                                                                                                           gaaTAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTtg                                                                   >  1:434183/1‑251 (MQ=255)                                                                                                                                                                            aataatATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTtgt                                                                  >  1:750027/1‑251 (MQ=255)                                                                                                                                                                             ataataTTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTtgtg                                                                 >  1:180914/1‑251 (MQ=255)                                                                                                                                                                               aataTTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAAt                                                                                                         <  1:855429/209‑1 (MQ=255)                                                                                                                                                                               aataTTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAAtttt                                                                                    >  1:18697/1‑230 (MQ=255)                                                                                                                                                                                 tatTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCAt                                                             >  1:61966/1‑251 (MQ=255)                                                                                                                                                                                                    ccTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATcc                                                                      >  1:87250/1‑223 (MQ=255)                                                                                                                                                                                                    ccTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGcc                                                               >  1:781555/1‑230 (MQ=255)                                                                                                                                                                                                         tgatTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCg                                     <  1:267625/251‑1 (MQ=255)                                                                                                                                                                                                           atTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATcc                                                                      >  1:859151/1‑216 (MQ=255)                                                                                                                                                                                                                    ttttAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAg                          >  1:716307/1‑251 (MQ=255)                                                                                                                                                                                                                    ttttAATACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAg                          <  1:624520/251‑1 (MQ=255)                                                                                                                                                                                                                          tACGAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAGTTAttt                    <  1:178787/251‑1 (MQ=255)                                                                                                                                                                                                                             gAAATAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGt                                                                                                                                   >  1:75816/1‑137 (MQ=255)                                                                                                                                                                                                                              aaaTAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGtttttt                                                                                                                                                                                                             >  1:773718/1‑63 (MQ=255)                                                                                                                                                                                                                              aaaTAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAg                                                                                                                                >  1:270440/1‑139 (MQ=255)                                                                                                                                                                                                                              aaaTAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTc                                                                                                     <  1:633720/166‑1 (MQ=255)                                                                                                                                                                                                                              aaaTAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAAtt                                                                                >  1:31331/1‑187 (MQ=255)                                                                                                                                                                                                                              aaaTAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGc                                                                <  1:110918/203‑1 (MQ=255)                                                                                                                                                                                                                              aaaTAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAGTTATTTTGtt                >  1:707292/1‑251 (MQ=255)                                                                                                                                                                                                                              aaaTAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAGTTATTTTGtt                >  1:702212/1‑251 (MQ=255)                                                                                                                                                                                                                              aaaTAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAGTTATTTTGtt                >  1:865529/1‑251 (MQ=255)                                                                                                                                                                                                                               aaTAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAGTTATTTTGTTc               <  1:581218/251‑1 (MQ=255)                                                                                                                                                                                                                               aaTAAGGCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAGTTATTTTGTTc               <  1:193892/251‑1 (MQ=255)                                                                                                                                                                                                                                    ggCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGcc                                                               >  1:446451/1‑198 (MQ=255)                                                                                                                                                                                                                                    ggCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAGTTATTTTGTTCCTTg           >  1:524173/1‑250 (MQ=255)                                                                                                                                                                                                                                     gCTGATCATTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGcgcg                                                                                                                                                                                  <  1:880487/83‑1 (MQ=255)                                                                                                                                                                                                                                            aTTTTTTGTGGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCATTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAGTTATTTTGTTCCTTGCCGAAAAgg  <  1:395364/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   ACTTGACGAAAAAACATGACTGAGATCGCATTTTAAGC‑AAAAAAATCGGCACCGAAGTGCCGACAATCCCAATCTATTTTATGTATATTATACGGTAAGATCCCGAATATCTTCGCTGGAAGCGTTGTTCATTACGGACTCTACACCTTTTTGTGCAGCAGCTTGCGAAGAATAATATTGGCTGCGACCGATTTCCTGATGATTTGCCGCTTTTAATACGAAATAAGGCTGATCATTTTTTGTAGTACGAAATTCAAAGTTTTTTGCATCCACTCCGTTTTTTTGCACGGAGGCAATGCCGTTTTGCGCGGAGGCTTTC‑GATTTGTAAAGCTCGCTGGTTAAAATAATTTGTCCGTTAGTTGCCATTAAATTGAACATAAATTGTCCATCTTTTGCTAATTTTAATTCGTAATATCCTTGTGCCATTTTAATTTCTCCCGGGTTTAATCGTTACTTAACAGTTATTTTGTTCCTTGCCGAAAAGG  >  1/1450711‑1451195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑