Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,443,318	T→C	intergenic (‑22305/+928)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,443,318	0	T	C	100.0%	206.4 / NA	74	intergenic (‑22305/+928)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/34);  total (40/34)

ATATATCGGCGTCGGCCTAGGCACCGCCGCTTTTGTGGCGTTTATGGCACGGGAAACTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAATGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAATTTATTATGAATAAATTTGCTA  >  1/1443078‑1443559                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  atatatCGGCGTCGGCCTAGGCACCGCCGCTTTTGTGGCGTTTATGGCACGGGAAACTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACgg                                                                                                                                                                                                                                         >  1:301961/1‑251 (MQ=255)                              cTTTTGTGGCGTTTATGGCACGGGAAACTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACAtttt                                                                                                                                                                                                            >  1:431437/1‑251 (MQ=255)                                        gTTTATGGCACGGGAAACTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTa                                                                                                                                                                                                  >  1:718493/1‑251 (MQ=255)                                           tATGGCACGGGAAACTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGc                                                                                                                                                                                               <  1:861067/251‑1 (MQ=255)                                           tATGGCACGGGAAACTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGc                                                                                                                                                                                               <  1:257110/251‑1 (MQ=255)                                           tATGGCACGGGAAACTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGc                                                                                                                                                                                               <  1:708377/251‑1 (MQ=255)                                           tATGGCACGGGAAACTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGc                                                                                                                                                                                               <  1:761397/251‑1 (MQ=255)                                                    ggAAACTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATAcg                                                                                                                                                                                      >  1:647831/1‑251 (MQ=255)                                                        aCTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGcgac                                                                                                                                                                                  <  1:212801/251‑1 (MQ=255)                                                                gCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAgg                                                                                                                                                                                                        <  1:454230/221‑1 (MQ=255)                                                                      cacCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACa                                                                                                                                                                    <  1:857976/251‑1 (MQ=255)                                                                           ccACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACa                                                                                                                                                                    <  1:785576/246‑1 (MQ=255)                                                                                  aCTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATcc                                                                                                                                                        <  1:680535/251‑1 (MQ=255)                                                                                           aTTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACtt                                                                                                                                               >  1:511484/1‑251 (MQ=255)                                                                                             ttACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACtt                                                                                                                                               >  1:524243/1‑249 (MQ=255)                                                                                             ttACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACtt                                                                                                                                               >  1:127421/1‑249 (MQ=255)                                                                                              tACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTtctc                                                                                                                                            <  1:239239/251‑1 (MQ=255)                                                                                                    ccTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAAt                                                                                                                                      <  1:204105/251‑1 (MQ=255)                                                                                                       gTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGc                                                                                                                                                                                               >  1:587478/1‑191 (MQ=255)                                                                                                       gTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATaaa                                                                                                                                   >  1:471885/1‑251 (MQ=255)                                                                                                               tGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAg                                                                                                                           <  1:227294/251‑1 (MQ=255)                                                                                                                    aGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAAc                                                                                                                                                                                            >  1:625721/1‑181 (MQ=255)                                                                                                                    aGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACa                                                                                                                                                                    >  1:200138/1‑205 (MQ=255)                                                                                                                          gggTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGttt                                                                                                                >  1:736971/1‑251 (MQ=255)                                                                                                                              tCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGtttttt                                                                                                             >  1:635423/1‑250 (MQ=255)                                                                                                                                 gAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCGTACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTtc                                                                                                                                              >  1:435139/1‑214 (MQ=255)                                                                                                                                 gAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCGTACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAg                                                                                                         >  1:454187/1‑251 (MQ=255)                                                                                                                                 gAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAg                                                                                                         >  1:820238/1‑251 (MQ=255)                                                                                                                                 gAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAg                                                                                                         >  1:213450/1‑251 (MQ=255)                                                                                                                                      ctctCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACAt                                                                                                                                                                   >  1:232491/1‑188 (MQ=255)                                                                                                                                      ctctCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGttt                                                                                                    >  1:73028/1‑251 (MQ=255)                                                                                                                                          cGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTAt                                                                                                >  1:408127/1‑251 (MQ=255)                                                                                                                                          cGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTAt                                                                                                >  1:501042/1‑251 (MQ=255)                                                                                                                                            ggCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTAtc                                                                                               <  1:775947/250‑2 (MQ=255)                                                                                                                                            ggCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTg                                                                                              >  1:188873/1‑251 (MQ=255)                                                                                                                                             gCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTg                                                                                              <  1:673723/250‑1 (MQ=255)                                                                                                                                             gCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTg                                                                                              <  1:347327/250‑1 (MQ=255)                                                                                                                                              cTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATcc                                                                                                                                                        <  1:656027/191‑1 (MQ=255)                                                                                                                                                    ggTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCtcatc                                                                                                                                         >  1:297533/1‑200 (MQ=255)                                                                                                                                                     gTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAag                                                                                      <  1:554531/250‑1 (MQ=255)                                                                                                                                                         aaGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGac                                                                                 <  1:476340/251‑1 (MQ=255)                                                                                                                                                             cGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGacaaca                                                                             >  1:825714/1‑251 (MQ=255)                                                                                                                                                                 ggTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGTAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGaa                                                                         >  1:747242/1‑251 (MQ=255)                                                                                                                                                                    tattatGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCAGCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAat                                                                      <  1:828596/251‑1 (MQ=255)                                                                                                                                                                         tGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTc                                                                 <  1:471211/251‑1 (MQ=255)                                                                                                                                                                               tttCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAg                                                           >  1:894098/1‑251 (MQ=255)                                                                                                                                                                                 tCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGt                                                         <  1:559830/251‑1 (MQ=255)                                                                                                                                                                                    ggATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGac                                                                                 <  1:647368/224‑1 (MQ=255)                                                                                                                                                                                    ggATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCa                                                      >  1:399993/1‑251 (MQ=255)                                                                                                                                                                                      aTTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCAc                                                     <  1:270355/250‑1 (MQ=255)                                                                                                                                                                                      aTTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCAc                                                     <  1:676744/250‑1 (MQ=255)                                                                                                                                                                                         tGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCggg                                                 >  1:689420/1‑251 (MQ=255)                                                                                                                                                                                          gTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGac                                                                                 >  1:391001/1‑218 (MQ=255)                                                                                                                                                                                                   tCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATcc                                                                                                                                                        >  1:125370/1‑138 (MQ=255)                                                                                                                                                                                                   tCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGaaaa                                       >  1:145036/1‑251 (MQ=255)                                                                                                                                                                                                      ccGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAgg                                     <  1:298811/250‑1 (MQ=255)                                                                                                                                                                                                         tACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTac                                  <  1:345330/250‑1 (MQ=255)                                                                                                                                                                                                             gggTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAg                             >  1:108397/1‑251 (MQ=255)                                                                                                                                                                                                             gggTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAg                             >  1:25198/1‑251 (MQ=255)                                                                                                                                                                                                              ggTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAg                             <  1:199871/250‑1 (MQ=255)                                                                                                                                                                                                                  tGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCaa                        >  1:855847/1‑251 (MQ=255)                                                                                                                                                                                                                  tGCTTTGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAG‑GGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAAt                       >  1:609134/1‑251 (MQ=255)                                                                                                                                                                                                                     tttGCCTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAAttt                     <  1:525933/251‑1 (MQ=255)                                                                                                                                                                                                                         ccTGTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAATttatta                 <  1:213532/251‑1 (MQ=255)                                                                                                                                                                                                                            gTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTacac                                <  1:285960/233‑1 (MQ=255)                                                                                                                                                                                                                            gTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAg                             >  1:60936/1‑236 (MQ=255)                                                                                                                                                                                                                            gTTTTGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAATTTATTATGa              >  1:509783/1‑251 (MQ=255)                                                                                                                                                                                                                              tttGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAATTTATTATGAAt            <  1:20202/251‑1 (MQ=255)                                                                                                                                                                                                                              tttGGGTCGCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAATTTATTATGAAt            <  1:135546/251‑1 (MQ=255)                                                                                                                                                                                                                                      gCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAg                                                                                                                           >  1:154934/1‑132 (MQ=255)                                                                                                                                                                                                                                      gCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAATTTATTATGAATAAATTTg     >  1:16912/1‑250 (MQ=255)                                                                                                                                                                                                                                      gCCCCATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAATTTATTATGAATAAATTTg     >  1:148690/1‑250 (MQ=255)                                                                                                                                                                                                                                        cccATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAATTTATTATGAATAAATTTGCTa  <  1:858563/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccATGGAACGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCtcatc                                                                                                                                         <  1:506244/115‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  ATATATCGGCGTCGGCCTAGGCACCGCCGCTTTTGTGGCGTTTATGGCACGGGAAACTAACCCGCTTTACACCGCCACCCAACTCGCCTTATTTACCAGCCTGTCTGCATTGCCAAGCAAAGGGTTCGGAATACTCTCGGGCTATTTGGTCAAAGCCGTCGGTTATTATGACTTTTTCTGGATTTGTTTATTGCTCGCCGTACCGGGTATGCTTTGCCTGTTTTGGGTCGCCCCATGGAATGAAAAAACGGTAGCGGCAAAATCCGACAATTGACATTTTCAGGGAATTAGGCAACCATACGCGACGATTTACTAAAACATCTTCAAAATCCACCGCACTTCTCATCAATAAACCAAAAAGTGCGGTGGTTTTTTCAAGCGTTTTTATTGACATAAAGAGGACAACATGAAAATTATTCTATTAGGTGCACCGGGCGCAGGAAAAGGTACACAAGCCCAATTTATTATGAATAAATTTGCTA  >  1/1443078‑1443559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑