Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,437,674	T→C	intergenic (‑16661/+6572)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,437,674	0	T	C	100.0%	184.1 / NA	66	intergenic (‑16661/+6572)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/25);  total (41/25)

ACTTTCAGTTGCGCGCTATTCACATTCACCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACTTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATACGACCGCAATTTTCTGCGCAATGTGGTGTTGCCTGAATTTCGCCAACGCTGGGCG  >  1/1437430‑1437914                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 aCTTTCAGTTGCGCGCTATTCACATTCACCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACctttttt                                                                                                                                                                                                                                            >  1:728631/1‑251 (MQ=255)                cTATTCACATTCACCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCTGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTc                                                                                                                                                                                                                                           <  1:179547/237‑1 (MQ=255)                cTATTCACATTCACCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAAcg                                                                                                                                                                                                                              >  1:834139/1‑250 (MQ=255)                  attcacattcacCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCt                                                                                                                                                                                                                                          <  1:872845/236‑1 (MQ=255)                  attcacattcacCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCgg                                                                                                                                                                                                                           >  1:7374/1‑251 (MQ=255)                  attcacattcacCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCgg                                                                                                                                                                                                                           >  1:668819/1‑251 (MQ=255)                      acattcacCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAg                                                                                                                                                                                                                        >  1:752320/1‑250 (MQ=255)                        attcacCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCgg                                                                                                                                                                                                                     <  1:469192/251‑1 (MQ=255)                           cacCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGttt                                                                                                                                                                                                                  >  1:413698/1‑251 (MQ=255)                           cacCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGttt                                                                                                                                                                                                                  >  1:420971/1‑251 (MQ=255)                           cacCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGttt                                                                                                                                                                                                                  >  1:569425/1‑251 (MQ=255)                           cacCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGttt                                                                                                                                                                                                                  >  1:575324/1‑251 (MQ=255)                             ccATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTg                                                                                                                                                                                                                 >  1:732467/1‑250 (MQ=255)                               aTGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAg                                                                                                                                                                                                              <  1:198374/251‑1 (MQ=255)                                        gCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGTACGGGTTAGGt                                                                                                                                                                                                     >  1:294865/1‑251 (MQ=255)                                        gCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAggg                                                                                                                                                                                                     >  1:743486/1‑250 (MQ=255)                                         cccTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGt                                                                                                                                                                                                     <  1:385262/250‑1 (MQ=255)                                          ccTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGtgc                                                                                                                                                                                                   <  1:377558/251‑1 (MQ=255)                                           cTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGtgca                                                                                                                                                                                                  >  1:672422/1‑251 (MQ=255)                                               tGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGtgcaatgc                                                                                                                                                                                              <  1:112259/251‑1 (MQ=255)                                               tGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGtgcaatgc                                                                                                                                                                                              <  1:413192/251‑1 (MQ=255)                                                     tGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTa                                                                                                                                                                                        >  1:361765/1‑251 (MQ=255)                                                     tGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTa                                                                                                                                                                                        >  1:297396/1‑251 (MQ=255)                                                            aCGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCAt                                                                                                                                                                                 <  1:827578/251‑1 (MQ=255)                                                               gCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAg                                                                                                                                                                                    >  1:590365/1‑245 (MQ=255)                                                                    ttGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCTGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACg                                                                                                                                                                         <  1:429338/251‑1 (MQ=255)                                                                     tGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACgg                                                                                                                                                                        <  1:737559/251‑1 (MQ=255)                                                                      gCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGtgc                                                                                                                                                                                                   <  1:293251/223‑1 (MQ=255)                                                                                  gCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACgg                                                                                                                                                                        >  1:615280/1‑238 (MQ=255)                                                                                       cAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTgcgc                                                                                                                                                                                                                                     <  1:461615/172‑1 (MQ=255)                                                                                       cAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGtgcaatgc                                                                                                                                                                                              <  1:337088/211‑1 (MQ=255)                                                                                       cAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGcc                                                                                                                                                       >  1:845346/1‑250 (MQ=255)                                                                                              atatTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGa                                                                                                                                                                <  1:414849/234‑1 (MQ=255)                                                                                                 ttCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCGTCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAAt                                                                                                                                            <  1:772979/251‑1 (MQ=255)                                                                                                 ttCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAAt                                                                                                                                            >  1:621361/1‑251 (MQ=255)                                                                                                       tGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAg                                                                                                                                       >  1:189273/1‑250 (MQ=255)                                                                                                           gATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAAttt                                                                                                                                          >  1:863813/1‑243 (MQ=255)                                                                                                              tCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGc                                                                                                                               <  1:632863/251‑1 (MQ=255)                                                                                                                     gTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGAt                                                                                                                        >  1:140881/1‑251 (MQ=255)                                                                                                                            tGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCAtt                                                                                                                  <  1:667402/250‑1 (MQ=255)                                                                                                                                        aCGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTgcaacagcaa                                                                                                     >  1:871458/1‑251 (MQ=255)                                                                                                                                         cGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTgcaacagcaac                                                                                                    <  1:581414/251‑1 (MQ=255)                                                                                                                                           gCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGcaacagcaacag                                                                                                  >  1:115051/1‑251 (MQ=255)                                                                                                                                                         gCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGa                                                                                    >  1:708553/1‑251 (MQ=255)                                                                                                                                                                     cGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTaa                                                                        >  1:234875/1‑251 (MQ=255)                                                                                                                                                                      gTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTaa                                                                        >  1:138479/1‑250 (MQ=255)                                                                                                                                                                      gTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTaa                                                                        >  1:34119/1‑250 (MQ=255)                                                                                                                                                                       ttATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAg                                                                                                                                                                                    <  1:69923/141‑1 (MQ=255)                                                                                                                                                                       ttATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCaacagcaacagc                                                                                                 <  1:368216/224‑1 (MQ=255)                                                                                                                                                                       ttATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACg                                                                      >  1:511163/1‑251 (MQ=255)                                                                                                                                                                       ttATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACg                                                                      >  1:578609/1‑251 (MQ=255)                                                                                                                                                                       ttATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACg                                                                      >  1:598260/1‑251 (MQ=255)                                                                                                                                                                         aTGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGtgcaatgc                                                                                                                                                                                              >  1:791450/1‑129 (MQ=255)                                                                                                                                                                              gCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGAcaa                                                               >  1:620710/1‑251 (MQ=255)                                                                                                                                                                               ccATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGt                                                                          <  1:691110/239‑1 (MQ=255)                                                                                                                                                                                    gCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATa                                                         >  1:484398/1‑251 (MQ=255)                                                                                                                                                                                        aacaTATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATACGAc                                                     <  1:713076/251‑1 (MQ=255)                                                                                                                                                                                         acaTATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATACGAc                                                     >  1:577534/1‑250 (MQ=255)                                                                                                                                                                                         acaTATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATACGAc                                                     >  1:551626/1‑250 (MQ=255)                                                                                                                                                                                                   cccGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATACGACCGCAAtttt                                            >  1:56860/1‑249 (MQ=255)                                                                                                                                                                                                      gaagaaTGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACgg                                                                                                                                                                        >  1:404199/1‑122 (MQ=255)                                                                                                                                                                                                       aagaaTGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTgcaacag                                                                                                        <  1:341950/185‑1 (MQ=255)                                                                                                                                                                                                       aagaaTGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATACGACCGCAATTTTCTgcg                                       >  1:91676/1‑250 (MQ=255)                                                                                                                                                                                                                        gcgcACCATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATACGACCGCAATTTTCTGCGCAATGTGGTGTTGCCTGa                     >  1:892790/1‑251 (MQ=255)                                                                                                                                                                                                                             ccATCAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATACGACCGCAATTTTCTGCGCAATGTGGTGTTGCCTGAAttt                 >  1:671056/1‑250 (MQ=255)                                                                                                                                                                                                                                 cAGCAAGATCAAACGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTgcaacag                                                                                                        >  1:548053/1‑159 (MQ=255)                                                                                                                                                                                                                                           aaaCGGAAACCTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATACGACCGCAATTTTCTGCGCAATGTGGTGTTGCCTGAATTTCGCCAACGCTGGgcg  <  1:736795/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 ACTTTCAGTTGCGCGCTATTCACATTCACCATGGCTTAAGCCCTAATGCCGATGCCTGGACGGCACATTGCCAACAAATTTGCCGACAATTGAATATTCCGTTGCTGATTCAGCCGGTTAAAGTGCGTTTGCAAAACGGCATTGAAGCCGGTGCCCGTGAGGCACGTTATGAAGCCATCGCACAACATATTTTGCCCGAAGAATGGCTAGCCACGGCGCACCATCAGCAAGATCAAACGGAAACTTTTTTTCTTGCGCTCAAACGCGGTAGCGGTTTGCAGGGGTTAGGTGCAATGCAAGCTAACAGCATCGTTTACGGCGTACCGATTTTTCGCCCGCTATTGAATTTTAGCCGTCAGCAACTGATGGCATTCGTGCAACAGCAACAGCTGACTTGGGTTGAAGACGAAAGTAACGAAGACAATCAATACGACCGCAATTTTCTGCGCAATGTGGTGTTGCCTGAATTTCGCCAACGCTGGGCG  >  1/1437430‑1437914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑