Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,437,196	C→A	intergenic (‑16183/+7050)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,437,196	0	C	A	100.0%	214.0 / NA	75	intergenic (‑16183/+7050)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/37);  total (38/37)

CAATCAATTTGAAATGTTGCTTGCTACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCCCGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGTGGCTTGGACTCTACCGCCCTTCTCACCTTATTTGCCAAACACCGGGAAAATCGACCGCACTTTCAGTTG  >  1/1436959‑1437440                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     caatcaatTTGAAATGTTGCTTGCTACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCTGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTatca                                                                                                                                                                                                                                         <  1:435992/251‑1 (MQ=255)       atTTGAAATGTTGCTTGCTACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTatca                                                                                                                                                                                                                                         >  1:609851/1‑245 (MQ=255)                gTTGCTTGCTACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGc                                                                                                                                                                                                                           >  1:143557/1‑250 (MQ=255)                 ttgcttgcTACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGcc                                                                                                                                                                                                                          <  1:352090/250‑1 (MQ=255)                     ttgctACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAatat                                                                                                                                                                                                                     <  1:671149/251‑1 (MQ=255)                        ctACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTatca                                                                                                                                                                                                                                         >  1:326938/1‑228 (MQ=255)                        ctACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTat                                                                                                                                                                                                                                           >  1:97601/1‑226 (MQ=255)                        ctACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATaa                                                                                                                                                                                                                   <  1:333987/250‑1 (MQ=255)                        ctACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATaa                                                                                                                                                                                                                   <  1:566201/250‑1 (MQ=255)                          cccGAACAGGGCATTGGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGc                                                                                                                                                                                                                <  1:526136/250‑1 (MQ=255)                             gAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGcaca                                                                                                                                                                                                             >  1:684541/1‑251 (MQ=255)                             gAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGcaca                                                                                                                                                                                                             >  1:742193/1‑251 (MQ=255)                             gAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGcaca                                                                                                                                                                                                             >  1:630678/1‑251 (MQ=255)                                  gggCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACaaaaaa                                                                                                                                                                                                        >  1:73451/1‑251 (MQ=255)                                   ggcattgggcatttATCCCGTCCATTACGTCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACaaaaaa                                                                                                                                                                                                        <  1:894945/250‑1 (MQ=255)                                    gcatttggcatttATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAAt                                                                                                                                                                                                       <  1:856051/250‑1 (MQ=255)                                     catttggcatttATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATc                                                                                                                                                                                                      <  1:868103/250‑1 (MQ=255)                                           gcatttATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCcgcg                                                                                                                                                                                                                               <  1:822589/219‑1 (MQ=255)                                           gcatttATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATaa                                                                                                                                                                                                                   <  1:295158/231‑1 (MQ=255)                                           gcatttATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAAt                                                                                                                                                                                               >  1:593426/1‑251 (MQ=255)                                           gcatttATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAAt                                                                                                                                                                                               >  1:7770/1‑251 (MQ=255)                                            catttATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAAcc                                                                                                                                                                                                                                   >  1:589408/1‑214 (MQ=255)                                                      gTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTtc                                                                                                                                                                                     >  1:725914/1‑250 (MQ=255)                                                       tCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTtcc                                                                                                                                                                                    <  1:86312/250‑1 (MQ=255)                                                        ccATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTTACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACCAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTtcct                                                                                                                                                                                   >  1:455435/1‑250 (MQ=255)                                                        ccATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAAtata                                                                                                                                                                                                                    >  1:412405/1‑217 (MQ=255)                                                        ccATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTtcct                                                                                                                                                                                   >  1:457066/1‑250 (MQ=255)                                                        ccATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTtcct                                                                                                                                                                                   >  1:843659/1‑250 (MQ=255)                                                        ccATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTtcct                                                                                                                                                                                   >  1:813892/1‑250 (MQ=255)                                                         cATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAGATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTtcctc                                                                                                                                                                                  >  1:454610/1‑250 (MQ=255)                                                         cATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTatc                                                                                                                                                                                                                                          <  1:668569/194‑1 (MQ=255)                                                         cATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAAcc                                                                                                                                                                                                                                   >  1:677698/1‑201 (MQ=255)                                                         cATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATaa                                                                                                                                                                                                                   <  1:755129/217‑1 (MQ=255)                                                         cATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTtcctc                                                                                                                                                                                  >  1:519960/1‑250 (MQ=255)                                                           ttACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTatcatc                                                                                                                                                                                                                                       >  1:419721/1‑195 (MQ=255)                                                           ttACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATCTAAAGc                                                                                                                                                                                                                >  1:59037/1‑218 (MQ=255)                                                            tACATCCTTTCGACCACGAACCCGTGGGGGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGc                                                                                                                                                                              <  1:376261/251‑1 (MQ=255)                                                            tACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGc                                                                                                                                                                              <  1:351363/251‑1 (MQ=255)                                                             aCATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCg                                                                                                                                                                               >  1:826769/1‑249 (MQ=255)                                                                        cccACGAACCCTTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGc                                                                                                                                                                  <  1:238770/250‑1 (MQ=255)                                                                        aCCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGc                                                                                                                                                                  <  1:765309/251‑1 (MQ=255)                                                                        aCCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACA‑GGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAgg                                                                                                                                                                   >  1:366857/1‑249 (MQ=255)                                                                           aCGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGc                                                                                                                                                                                                                >  1:95489/1‑202 (MQ=255)                                                                            cGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATg                                                                                                                                                              <  1:81115/251‑1 (MQ=255)                                                                              aaCCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGt                                                                                                                                                             <  1:400625/250‑1 (MQ=255)                                                                              aaCCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGt                                                                                                                                                             <  1:447429/250‑1 (MQ=255)                                                                               aCCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTa                                                                                                                                                           <  1:258782/251‑1 (MQ=255)                                                                                      ggCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGGACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTa                                                                                                                                                    >  1:66616/1‑251 (MQ=255)                                                                                      ggCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATc                                                                                                                                                                                              <  1:56851/209‑1 (MQ=255)                                                                                      ggCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGt                                                                                                                                                             >  1:406613/1‑242 (MQ=255)                                                                                          tGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGc                                                                                                                                                 <  1:591660/250‑1 (MQ=255)                                                                                           gggTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCtt                                                                                                                                               >  1:588654/1‑251 (MQ=255)                                                                                                           ggCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTca                                                                                                                                >  1:353800/1‑250 (MQ=255)                                                                                                           ggCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTca                                                                                                                                >  1:268772/1‑250 (MQ=255)                                                                                                           ggCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTca                                                                                                                                >  1:277822/1‑250 (MQ=255)                                                                                                             cTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTcaca                                                                                                                              <  1:561005/250‑1 (MQ=255)                                                                                                                        ccAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAAc                                                                                                                   >  1:379531/1‑250 (MQ=255)                                                                                                                         cAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACgg                                                                                                                 <  1:476266/251‑1 (MQ=255)                                                                                                                          aaTTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTa                                                                                                                                                           >  1:736655/1‑208 (MQ=255)                                                                                                                                                     gAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTtctc                                                                                      <  1:885773/250‑1 (MQ=255)                                                                                                                                                     gAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTtctc                                                                                      <  1:665765/250‑1 (MQ=255)                                                                                                                                                                acacATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGc                                                                          <  1:361962/251‑1 (MQ=255)                                                                                                                                                                   caTGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGt                                                                       <  1:235294/251‑1 (MQ=255)                                                                                                                                                                     tGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATg                                                                                                                                                              >  1:527992/1‑162 (MQ=255)                                                                                                                                                                                gTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGt                                                                                                                                                             <  1:302398/152‑1 (MQ=255)                                                                                                                                                                                 tCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGTGGCTTGGACTCTAc                                                         <  1:724590/251‑1 (MQ=255)                                                                                                                                                                                  cAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCtt                                                                                                                                                                                                                                             >  1:861578/1‑70 (MQ=255)                                                                                                                                                                                               cAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGTGGCTTGGACTCTACCGCCCTTCTCAcc                                            <  1:227961/250‑1 (MQ=255)                                                                                                                                                                                                     accCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACgg                                                                                                                 <  1:523705/175‑1 (MQ=255)                                                                                                                                                                                                     accCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGTGGCTTGGACTCTACCGCCCTTCTCACCTTATTTg                                     >  1:739198/1‑251 (MQ=255)                                                                                                                                                                                                                      aTACGAATTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGTGGCTTGGACTCTACCGCCCTTCTCACCTTATTTGCCAAACACCGGGAAAAt                    <  1:391221/251‑1 (MQ=255)                                                                                                                                                                                                                           aaTTACAAATCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGTGGCTTGGAc                                                              >  1:38115/1‑204 (MQ=255)                                                                                                                                                                                                                                  aaTCATCGCCGCACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGTGGCTTGGACTCTACCGCCCTTCTCACCTTATTTGCCAAACACCGGGAAAATCGACCGCACttt        >  1:615724/1‑251 (MQ=255)                                                                                                                                                                                                                                        cgccgcACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGTGGCTTGGACTCTACCGCCCTTCTCACCTTATTTGCCAAACACCGGGAAAATCGACCGCACTTTCAGTTg  <  1:552884/251‑1 (MQ=255)                                                                                                                                                                                                                                        cgccgcACGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGTGGCTTGGACTCTACCGCCCTTCTCACCTTATTTGCCAAACACCGGGAAAATCGACCGCACTTTCAGTTg  <  1:686151/251‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                     CAATCAATTTGAAATGTTGCTTGCTACCGAACAGGGCATTTGGCATTTATCCCGTCCATTACATCCGTTCGACCACGAACCCGTTGGCGTGGGTGATTTAACCGCCGGCTTATTCTTAGCCAATTTACTCAACGGCAAATCCGATGTGGAGGCTTTTGAACACATGGCAAACGCAGTCAACGATGTGATGCAAACCACCCTGGAACGTGGCGAATACGAATTACAAATCATCGCCGCCCGCGATCTTATCATCAACCCGCGCAGCCAATATAAAGCACAAAAAATCGCTTAATCTGCCACTTCCTCCCGCTTAGCGGGAGGCTATGTTATCCGCTATGCTTCTCGAACAATTCTCACAGCAGTTACAACGGCACGCACCAAATCAAACGCAATTTCTCATTGGATTTAGCGGTGGCTTGGACTCTACCGCCCTTCTCACCTTATTTGCCAAACACCGGGAAAATCGACCGCACTTTCAGTTG  >  1/1436959‑1437440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑