Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,433,757	A→G	intergenic (‑12744/+10489)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,433,757	0	A	G	100.0%	153.8 / NA	53	intergenic (‑12744/+10489)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/25);  total (28/25)

GACAAATACATAATGTCACGTGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAATTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATGGGTAAAAACTCTTCGTATATAATTGTTCGAGATATTGCTTTACCTGAAACTTTAGTTTATAGATACCTTTATTATCT  >  1/1433567‑1433963                                                                                                                                                                                               |                                                                                                                                                                                                               gACAAATACATAATGTCACGTGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTc                                                                                                                                                                                                  >  1:544408/1‑205 (MQ=255) gACAAATACATAATGTCACGTGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGt                                                                                                                                                               >  1:516031/1‑240 (MQ=255) gACAAATACATAATGTCACGTGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCAt                                                                                                                                                    >  1:52464/1‑251 (MQ=255) gACAAATACATAATGTCACGTGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCAt                                                                                                                                                    >  1:220753/1‑251 (MQ=255)   cAAATACATAATGTCACGTGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATaa                                                                                                                                                  >  1:345058/1‑251 (MQ=255)     aaTACATAATGTCACGTGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAgg                                                                                                                                                <  1:501073/251‑1 (MQ=255)         cATAATGTCACGTGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTCTCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGAc                                                                                                                                            <  1:566751/251‑1 (MQ=255)             aTGTCACGTGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTaga                                                                                                                                                            <  1:568646/231‑1 (MQ=255)                     tGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTag                                                                                                                                                             >  1:691271/1‑222 (MQ=255)                       aTTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCt                                                                                                                                                                                <  1:613499/201‑1 (MQ=255)                            aTACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGAt                                                                                                                         >  1:726996/1‑251 (MQ=255)                                 aTCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGt                                                                                                                                                          <  1:134764/213‑1 (MQ=255)                                   ctcCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATgcgc                                                                                                                  <  1:831705/251‑1 (MQ=255)                                     ccTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCAt                                                                                                                >  1:530877/1‑251 (MQ=255)                                     ccTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCAt                                                                                                                >  1:49799/1‑251 (MQ=255)                                                 aaaaTTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAAGCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAAtt                                                                                                    <  1:50833/251‑1 (MQ=255)                                                       aCTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTA‑ATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAgg                                                                                             >  1:615208/1‑251 (MQ=255)                                                          ttATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATc                                                                                                                                   <  1:28982/211‑1 (MQ=255)                                                                                cGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTatat                                                                                                                                                                 <  1:112907/159‑1 (MQ=255)                                                                                  cTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATgc                                                                                                                    >  1:497108/1‑202 (MQ=255)                                                                                     gTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAgtg                                                                                                                                        >  1:553417/1‑179 (MQ=255)                                                                                     gTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATgc                                                                                                                    <  1:848993/199‑1 (MQ=255)                                                                                     gTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCaa                                                                                     <  1:612823/230‑1 (MQ=255)                                                                                      tACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATGGGTAAAAACTCTTc                                                                 <  1:890441/249‑1 (MQ=255)                                                                                          aTTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGcac                                                                                                                              >  1:254797/1‑184 (MQ=255)                                                                                          aTTCATAAGTCATAACATAATACTACATACCT‑AATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTatat                                                                                                                                                                 >  1:866182/1‑148 (MQ=255)                                                                                                  gTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATaaa                                                                                 <  1:336863/221‑1 (MQ=255)                                                                                                  gTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATGGGTAAAAACTCTTCGTATATAATTGtt                                                    >  1:833607/1‑250 (MQ=255)                                                                                                   tCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAAc                                                                                                                                                                                     >  1:826711/1‑120 (MQ=255)                                                                                                        acataaTACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCt                                                                                                                                                                           >  1:15123/1‑125 (MQ=255)                                                                                                        acataaTACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGAc                                                                                                                                            >  1:168132/1‑156 (MQ=255)                                                                                                        acataaTACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATGGGTAAAAAc                                                                      >  1:147073/1‑226 (MQ=255)                                                                                                         cataaTACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATGGGTAAAAACTCTTCGTATATAAt                                                        <  1:676380/239‑1 (MQ=255)                                                                                                           taataCTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACct                                                                                                                                                                       >  1:737513/1‑126 (MQ=255)                                                                                                           taataCTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATGGGTAAAAACTCTTCGTATATAATTGTTCGAGATATTg                                          >  1:192767/1‑251 (MQ=255)                                                                                                           taataCTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATGGGTAAAAACTCTTCGTATATAATTGTTCGAGATATTg                                          >  1:709678/1‑251 (MQ=255)                                                                                                           taataCTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTA‑CAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATgcg                                                                                                                   >  1:690853/1‑177 (MQ=255)                                                                                                             ataCTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCt                                                                                                                                                                                >  1:389867/1‑115 (MQ=255)                                                                                                             ataCTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATaaa                                                                                 >  1:604988/1‑210 (MQ=255)                                                                                                                       ccTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACct                                                                                                                                                                       <  1:755551/114‑1 (MQ=255)                                                                                                                       ccTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTagag                                                                                                                                                           <  1:324529/126‑1 (MQ=255)                                                                                                                       ccTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGAt                                                                                                                         <  1:60492/160‑1 (MQ=255)                                                                                                                       ccTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCAt                                                                                                                <  1:484911/169‑1 (MQ=255)                                                                                                                        cTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAAcc                                                                                                                                                      <  1:279745/130‑1 (MQ=255)                                                                                                                            atatTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATg                                                                               <  1:645682/197‑1 (MQ=255)                                                                                                                                    tCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATGGGTAAAAACTCTTCGTATATAATTGTTCGAGATATTGCTTTACCTGAAACTTTAGTTTATAg                 <  1:330110/251‑1 (MQ=255)                                                                                                                                                     gTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATg                                                                                                                                                                <  1:261272/91‑1 (MQ=255)                                                                                                                                                     gTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCaa                                                                                     <  1:22056/166‑1 (MQ=255)                                                                                                                                                         aGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGt                                                                                                                                                               >  1:25601/1‑88 (MQ=255)                                                                                                                                                         aGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCttt                                                                                                        >  1:165092/1‑143 (MQ=255)                                                                                                                                                         aGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCaa                                                                                     <  1:778522/162‑1 (MQ=255)                                                                                                                                                         aGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGCACTTCAATAAATGGGTAAAAACTCTTCGTATATAATTGTTCGAGATATTGCTTTACCTGAAACTTTAGTTTATAGATACCTTTATTATCt  >  1:672592/1‑245 (MQ=255)                                                                                                                                                                   cTGTGAAGGAAAATCTAAAAAACGCCTAGTTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATGGGTAAAAACTCTTCGTATATAAt                                                        <  1:478906/181‑1 (MQ=255)                                                                                                                                                                                               |                                                                                                                                                                                                               GACAAATACATAATGTCACGTGATTTCATACAATCTCCTTTAATTGTAAAAATTACTTTATCTACCTAAATTTTTCTTTCGCTAGTACTATTCATAAGTCATAACATAATACTACATACCTAAATATTAGCTCGACACCAGCCAGCCAGTCTAGCAATGACACTGTGAAGGAAAATCTAAAAAACGCCTAATTAGGCGTTTTTTCTTTACCTAAGAACAAGCTCAGCTACCTCTATATGTAGAGTAACCATAAGGAGACAGTGTAATCGGCACATGATAATGCGCATTATCCTTTAATTCGAAAGGTACTTCAATAAATGGGTAAAAACTCTTCGTATATAATTGTTCGAGATATTGCTTTACCTGAAACTTTAGTTTATAGATACCTTTATTATCT  >  1/1433567‑1433963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑