Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,432,815	T→C	intergenic (‑11802/+11431)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,432,815	0	T	C	100.0%	147.0 / NA	54	intergenic (‑11802/+11431)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/29);  total (25/29)

GACTAATTTAAGCAGCCTTTTCCCTTAAAATAAAAACTGACTAACTCTGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAATGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTAACTTTCTATTAAGCTTTATAGCCTCGTCATAGGTAACAGGACGTCTTT  >  1/1432579‑1433045                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                       gACTAATTTAAGCAGCCTTTTCCCTTAAAATAAAAACTGACTAACTCTGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGat                                                                                                                                                                                                                            <  1:112255/249‑1 (MQ=255)     aaTTTAAGCAGCCTTTTCCCTTAAAATAAAAACTGACTAACTCTGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGata                                                                                                                                                                                                                           >  1:839696/1‑246 (MQ=255)     aaTTTAAGCAGCCTTTTCCCTTAAAATAAAAACTGACTAACTCTGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCa                                                                                                                                                                                                                        >  1:866011/1‑249 (MQ=255)                ccTTTTCCCTTAAAATAAAAACTGACTAACTCTGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTAcc                                                                                                                                                                                                             <  1:622286/249‑1 (MQ=255)                ccTTTTCCCTTAAAATAAAAACTGACTAACTCTGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTAcc                                                                                                                                                                                                             <  1:364487/249‑1 (MQ=255)                             aataaaaACTGACTAACTCTGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGtttt                                                                                                                                                                                                                 <  1:9829/232‑1 (MQ=255)                                     cTGACTAACTCTGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATAtt                                                                                                                                                                                      >  1:155482/1‑251 (MQ=255)                                            aCTCTGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGa                                                                                                                                                                                                                             >  1:783550/1‑205 (MQ=255)                                             ctctGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGAt                                                                                                                                                                              <  1:740289/251‑1 (MQ=255)                                                        tAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGt                                                                                                                                                                   <  1:751451/251‑1 (MQ=255)                                                              tAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAgg                                                                                                                                                             >  1:838410/1‑251 (MQ=255)                                                                 aaCTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGa                                                                                                                                                                                                                             >  1:364313/1‑184 (MQ=255)                                                                           aaGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTtgaactgaac                                                                                                                                                <  1:135344/251‑1 (MQ=255)                                                                             gagaTATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGt                                                                                                                                              >  1:818625/1‑251 (MQ=255)                                                                               gaTATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGAt                                                                                                                                                                              >  1:75250/1‑217 (MQ=255)                                                                               gaTATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTtgaactgaac                                                                                                                                                >  1:836147/1‑247 (MQ=255)                                                                                atatatTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGc                                                                                                                                           <  1:688253/251‑1 (MQ=255)                                                                                    atTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGGAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATAtt                                                                                                                                                                                      >  1:614114/1‑204 (MQ=255)                                                                                     ttttttAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGAt                                                                                                                                                                              <  1:375406/211‑1 (MQ=255)                                                                                              tGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGAt                                                                                                                                                                              >  1:163897/1‑202 (MQ=255)                                                                                               gTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAg                                                                                                                                                                    >  1:447837/1‑211 (MQ=255)                                                                                                                      cccTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCaaa                                                                                                     >  1:660457/1‑251 (MQ=255)                                                                                                                      cccTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCaaa                                                                                                     >  1:500779/1‑251 (MQ=255)                                                                                                                       ccTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAatc                                                                                                                         >  1:133300/1‑230 (MQ=255)                                                                                                                        cTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAAc                                                                                                   <  1:541083/251‑1 (MQ=255)                                                                                                                        cTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAAc                                                                                                   <  1:274492/251‑1 (MQ=255)                                                                                                                          tCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTc                                                                                                 <  1:436533/251‑1 (MQ=255)                                                                                                                                   tGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACttat                                                                                         <  1:21869/250‑1 (MQ=255)                                                                                                                                    gAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGa                                                                                                                                                                                                                             >  1:777058/1‑117 (MQ=255)                                                                                                                                                 atatTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTAccc                                                                                                                                                                                                            >  1:151572/1‑121 (MQ=255)                                                                                                                                                 atatTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTtgaactgaac                                                                                                                                                >  1:75379/1‑181 (MQ=255)                                                                                                                                                 atatTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCaa                                                                          <  1:623021/251‑1 (MQ=255)                                                                                                                                                  tatTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAAt                                                                         <  1:792060/251‑1 (MQ=255)                                                                                                                                                  tatTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAAt                                                                         <  1:377470/251‑1 (MQ=255)                                                                                                                                                     tCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAg                                                                      <  1:257317/251‑1 (MQ=255)                                                                                                                                                           acCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCa                                                                           <  1:28281/240‑1 (MQ=255)                                                                                                                                                                 cTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACt                                                                                                                                                                                                   >  1:621350/1‑114 (MQ=255)                                                                                                                                                                 cTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTaa                                                          >  1:769172/1‑251 (MQ=255)                                                                                                                                                                    ttATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACAt                                                       <  1:292001/251‑1 (MQ=255)                                                                                                                                                                          gCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGat                                                                                                                                                                                                                            <  1:665044/80‑1 (MQ=255)                                                                                                                                                                           cTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTAAc                                                <  1:413485/251‑1 (MQ=255)                                                                                                                                                                                    catcatAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTAACTTTCTATTa                                       <  1:735728/251‑1 (MQ=255)                                                                                                                                                                                      tcatAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACt                                                                                                                                                                                                   <  1:324019/93‑1 (MQ=255)                                                                                                                                                                                      tcatAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTtgaac                                                                                                                                                     >  1:367654/1‑139 (MQ=255)                                                                                                                                                                                      tcatAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACt                                                                                            >  1:243171/1‑196 (MQ=255)                                                                                                                                                                                      tcatAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAAc                                                         <  1:81218/231‑1 (MQ=255)                                                                                                                                                                                      tcatAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTAACTTTCTATTAAg                                     >  1:500738/1‑251 (MQ=255)                                                                                                                                                                                           aaGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTAACt                                               <  1:310008/236‑1 (MQ=255)                                                                                                                                                                                                  aaCGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTAACTTTCTATTAAGCTTTATAGCCTc                         <  1:716481/251‑1 (MQ=255)                                                                                                                                                                                                           aaaTCATTTTTAAAAAACAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTAACTTTCTATTAAGCTTTATAGCCTCGTCATAgg                 <  1:304505/250‑1 (MQ=255)                                                                                                                                                                                                                           aCAGAAGCCTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTAACTTTCTATTAAGCTTTATAGCCTCGTCATAGGTAACAGGACGtcttt  >  1:638544/1‑249 (MQ=255)                                                                                                                                                                                                                                  ccTCAAAACAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTaa                                                 <  1:80170/195‑1 (MQ=255)                                                                                                                                                                                                                                       aaaCAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAg                                                                                                                                                                    >  1:37702/1‑75 (MQ=255)                                                                                                                                                                                                                                       aaaCAACGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTAACTTTCTATTAAGCTTTATAGCCTCGTCATAGGTAACAgg          <  1:192562/229‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                       GACTAATTTAAGCAGCCTTTTCCCTTAAAATAAAAACTGACTAACTCTGGCTAATTAAAAATAAAACTAAAGCGAAGAGATATATTTTTTAACTGTCAAGTGAGCTCGCCCTTTTTCCCCTTCTAACAGGTGAAAATTATCATCATATTCAACAACCAAACTTTTATTTGCTTGTAATTCATCATAAAGCTGTAACGCAAAGAAATCATTTTTAAAAAACAGAAGCCTCAAAACAATGAGTGGTAAGATATCACTGTTTTTACCCAGTTTAACTAATTCTTGATATTCGTTAGATAATTTAAAAGTTGTAGGATTTGAACTGAACGTAGCCAATACATCAGACGAATCATCCCAAGTTTCTTTCCAAAACTCATACTTATTATCAAAGCTATCAATAAGCGCCTTAGGTAACATTACTAACTTTCTATTAAGCTTTATAGCCTCGTCATAGGTAACAGGACGTCTTT  >  1/1432579‑1433045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑