Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,432,157	C→A	intergenic (‑11144/+12089)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,432,157	0	C	A	100.0%	70.8 / NA	28	intergenic (‑11144/+12089)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/18);  total (10/18)

CTATTAAGTAAAGCTAAGGTGATTGAAGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAACGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGGGGAATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCA  >  1/1431979‑1432370                                                                                                                                                                                   |                                                                                                                                                                                                                      ctactAAGTAAAGCAAAGGTGATTGAAGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAAc                                                                                                                                                                                          >  1:208337/5‑208 (MQ=255)  tactAAGTAAAGCAAAGGTGATTGAAGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCg                                                                                                                                              <  1:787709/248‑1 (MQ=255)         tAAAGCAAAGGTGATTGAAGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGataaat                                                                                                                                        <  1:113098/250‑1 (MQ=255)           aaGCAAAGGTGATTGAAGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAAt                                                                                                                                                                                              >  1:730566/1‑194 (MQ=255)           aaGCAAAGGTGATTGAAGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACa                                                                                                                                                                                         >  1:78960/1‑199 (MQ=255)              caaaGGTGATTGAAGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCg                                                                                                                                   <  1:811968/248‑1 (MQ=255)                          aaGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttc                                                                                                                      <  1:534059‑M1/251‑8 (MQ=255)                                  aGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATTGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAAt                                                                                                                                                                                              <  1:361858/171‑1 (MQ=255)                                    cTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGataaat                                                                                                                                        >  1:186895/1‑223 (MQ=255)                                          cGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataatt                                                                                                      <  1:353600‑M1/251‑24 (MQ=255)                                                   aaTTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGCGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACa                                                                                                                                                                                         >  1:844659/1‑159 (MQ=255)                                                             atgatgAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatactt                                                                                                                       <  1:573166‑M1/215‑7 (MQ=255)                                                              tgatgaGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTc                                                                                                                                                                          >  1:558209/1‑163 (MQ=255)                                                                         ggTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACa                                                                                                                                                                                         <  1:374983/137‑1 (MQ=255)                                                                            aaaTTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCaa                                                                                                                                                                                  <  1:787692/141‑1 (MQ=255)                                                                                 ccccAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACgg                                                                                                                                                                                                      >  1:468394/1‑116 (MQ=255)                                                                                             tAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagc                                                   <  1:267202‑M1/251‑75 (MQ=255)                                                                                                          gggCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtag                                       <  1:469383‑M1/250‑87 (MQ=255)                                                                                                           ggCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtaggg                                     >  1:189809‑M1/1‑163 (MQ=255)                                                                                                              gtgtAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAAtt                                                                                                                                                                                <  1:454635/109‑1 (MQ=255)                                                                                                                    cGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttag                             <  1:209946‑M1/250‑97 (MQ=255)                                                                                                                      tGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcat                          <  1:51365‑M1/251‑100 (MQ=255)                                                                                                                        gagaAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataat                                                                                                       >  1:151176‑M1/1‑150 (MQ=255)                                                                                                                                   atatTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatg                    >  1:663575‑M1/1‑139 (MQ=255)                                                                                                                                         aTACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAAc                                                                                                                                                                                          <  1:772906/72‑1 (MQ=255)                                                                                                                                         aTACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccattt                                                                                                               <  1:94481‑M1/147‑15 (MQ=255)                                                                                                                                              tGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttatttaaattcgttttatataattaccgctcttacgtaattagcatacccaagtagggcactttagcatcaaatgtacaacatcgttcaacac  <  1:139352‑M1/251‑124 (MQ=255)                                                                                                                                                                       aTTTTCTTCCAAAGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGatacttccccatttcatataattttctgtttattta                                                                                         <  1:521727‑M1/139‑37 (MQ=255)                                                                                                                                                                                   |                                                                                                                                                                                                                      CTATTAAGTAAAGCTAAGGTGATTGAAGTCACCAGCTTGAGCGACACAACAATTTGGCGTATGATGAGACAAGGTAAATTCCCCAAAAGTGTTAAAGCGTCCATGGGGCGTGTAGCGTGGAGAAAATCTGATATTGATACTTGGCTTGCTGAACGTTCTTGATGGCATTTTCTTCCAACGACGGTAAAAATGACGGTATAGAATAAACAATATCAATTTTATTCATTGATAAATCATTGCATTATGTCGCCGATAAATAATCGAGTGGGGGGAATACTTCCCCATTTCATATAATTTTCTGTTTATTTAAATTCGTTTTATATAATTACCGCTCTTACGTAATTAGCATACCCAAGTAGGGCACTTTAGCATCAAATGTACAACATCGTTCA  >  1/1431979‑1432370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑