Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,429,449	C→T	intergenic (‑8436/+14797)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,429,449	0	C	T	100.0%	80.5 / NA	32	intergenic (‑8436/+14797)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/18);  total (14/18)

TTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTACTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCCGGTGAGTATGTGACTTGCCGGAAATACC  >  1/1429209‑1429523                                                                                                                                                                                                                                                 |                                                                           tttttGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTc                                                                  <  1:400948/251‑1 (MQ=255)            ccttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGtt                                                       >  1:484478/16‑251 (MQ=255)            ccttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGtt                                                       >  1:381610/16‑251 (MQ=255)             cttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTc                                                      <  1:880160/237‑1 (MQ=255)                  gtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgtt                                                 >  1:528246/10‑251 (MQ=255)                  gtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgtt                                                 >  1:538397/10‑251 (MQ=255)                   tctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgt                                                  <  1:301100/241‑1 (MQ=255)                   tctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtg                                                   <  1:771209/240‑1 (MQ=255)                         acaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTccg                                           >  1:391640/3‑250 (MQ=255)                                   tttaaTTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGcc                                <  1:868683/247‑1 (MQ=255)                                    ttaaTTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGccc                               <  1:603440/248‑1 (MQ=255)                                               ttCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                    <  1:294071/251‑1 (MQ=255)                                               ttCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                    <  1:123428/251‑1 (MQ=255)                                                 cccAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtgtg                  <  1:139580/251‑1 (MQ=255)                                                   cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTc                                                      <  1:785865/213‑1 (MQ=255)                                                   cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:795527/251‑1 (MQ=255)                                                   cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:168851/251‑1 (MQ=255)                                                   cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:217588/251‑1 (MQ=255)                                                   cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATCGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:576381/251‑1 (MQ=255)                                                             gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                    <  1:199358/237‑1 (MQ=255)                                                             gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGACttaacgtatt      >  1:313048/1‑243 (MQ=255)                                                             gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGCCATTGGCAAACGACCAAAGGCGGGGTTGTACATTCTGGGCGATAGCTTCAGATTCAGTTGCGTAGTTCGAACAACCTTGCCCAAACGGCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGGGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGTGTAtgtggtttaacgtctt      >  1:674304/1‑239 (MQ=255)                                                              attgacacCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgttg                                                >  1:754933/5‑208 (MQ=255)                                                                                  gctCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:801992/218‑1 (MQ=255)                                                                                                       gttctaaaTCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAt                     >  1:575264/8‑194 (MQ=255)                                                                                                                     aaCCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:468856/185‑1 (MQ=255)                                                                                                                                                      tGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTgtgg                                                           >  1:870998/1‑109 (MQ=255)                                                                                                                                                       gTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtgtg                  >  1:139432/1‑149 (MQ=255)                                                                                                                                                                             aGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGACttaacgtatttttc  >  1:757406/1‑131 (MQ=255)                                                                                                                                                                               aTTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtgtg                  >  1:667333/1‑125 (MQ=255)                                                                                                                                                                                                             cAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                >  1:189321/1‑97 (MQ=255)                                                                                                                                                                                                                       aTCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTc                                             <  1:4864/58‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                           TTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTACTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCCGGTGAGTATGTGACTTGCCGGAAATACC  >  1/1429209‑1429523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑