Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,429,387	G→A	intergenic (‑8374/+14859)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,429,387	0	G	A	100.0%	113.4 / NA	42	intergenic (‑8374/+14859)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/22);  total (20/22)

TTGATAGTCAATTGAGATTGTCAGCTTGCGCGAATCGGGTTCAAAATCCGCCTGATAATCGGTAATTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTACTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCCGGTGAGTATGTGACTTGCCGGAAATACC  >  1/1429144‑1429523                                                                                                                                                                                                                                                    |                                                                                                                                         ctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGt                                                                                                                                   <  1:288930/250‑1 (MQ=255)    ataatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAg                                                                                                                                 <  1:828515/246‑1 (MQ=255)      aatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAGACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGtt                                                                                                                               >  1:292221/3‑250 (MQ=255)                  ttGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTg                                                                                                                   >  1:653799/1‑250 (MQ=255)                                      ggTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGAtt                                                                                              >  1:334215/1‑251 (MQ=255)                                       gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAg                                                                                                                                 >  1:850259/1‑215 (MQ=255)                                       gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGAttt                                                                                             >  1:186166/1‑251 (MQ=255)                                        ttCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGt                                                                                                                                   >  1:642525/1‑212 (MQ=255)                                                           tCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                          <  1:707404/250‑4 (MQ=255)                                                           tCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                          <  1:636485/250‑4 (MQ=255)                                                           tCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGACCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCGATCGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCTAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                          <  1:545062/250‑4 (MQ=255)                                                                  tttttGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTc                                                                  <  1:400948/251‑1 (MQ=255)                                                                             ccttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGtt                                                       >  1:484478/16‑251 (MQ=255)                                                                             ccttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGtt                                                       >  1:381610/16‑251 (MQ=255)                                                                              cttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTc                                                      <  1:880160/237‑1 (MQ=255)                                                                                   gtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgtt                                                 >  1:538397/10‑251 (MQ=255)                                                                                   gtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgtt                                                 >  1:528246/10‑251 (MQ=255)                                                                                    tctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgt                                                  <  1:301100/241‑1 (MQ=255)                                                                                    tctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtg                                                   <  1:771209/240‑1 (MQ=255)                                                                                          acaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTccg                                           >  1:391640/3‑250 (MQ=255)                                                                                                    tttaaTTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGcc                                <  1:868683/247‑1 (MQ=255)                                                                                                     ttaaTTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGccc                               <  1:603440/248‑1 (MQ=255)                                                                                                                ttCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                    <  1:123428/251‑1 (MQ=255)                                                                                                                ttCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                    <  1:294071/251‑1 (MQ=255)                                                                                                                  cccAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtgtg                  <  1:139580/251‑1 (MQ=255)                                                                                                                    cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTc                                                      <  1:785865/213‑1 (MQ=255)                                                                                                                    cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:217588/251‑1 (MQ=255)                                                                                                                    cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:168851/251‑1 (MQ=255)                                                                                                                    cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:795527/251‑1 (MQ=255)                                                                                                                    cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATCGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:576381/251‑1 (MQ=255)                                                                                                                              gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                          >  1:162077/1‑180 (MQ=255)                                                                                                                              gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                    <  1:199358/237‑1 (MQ=255)                                                                                                                              gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGACttaacgtatt      >  1:313048/1‑243 (MQ=255)                                                                                                                              gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGCCATTGGCAAACGACCAAAGGCGGGGTTGTACATTCTGGGCGATAGCTTCAGATTCAGTTGCGTAGTTCGAACAACCTTGCCCAAACGGCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGGGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGTGTAtgtggtttaacgtctt      >  1:674304/1‑239 (MQ=255)                                                                                                                               attgacacCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgttg                                                >  1:754933/5‑208 (MQ=255)                                                                                                                                                   gctCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:801992/218‑1 (MQ=255)                                                                                                                                                                        gttctaaaTCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAt                     >  1:575264/8‑194 (MQ=255)                                                                                                                                                                                      aaCCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc                <  1:468856/185‑1 (MQ=255)                                                                                                                                                                                                                       tGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTgtgg                                                           >  1:870998/1‑109 (MQ=255)                                                                                                                                                                                                                        gTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtgtg                  >  1:139432/1‑149 (MQ=255)                                                                                                                                                                                                                                              aGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGACttaacgtatttttc  >  1:757406/1‑131 (MQ=255)                                                                                                                                                                                                                                                aTTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtgtg                  >  1:667333/1‑125 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                         TTGATAGTCAATTGAGATTGTCAGCTTGCGCGAATCGGGTTCAAAATCCGCCTGATAATCGGTAATTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTACTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCCGGTGAGTATGTGACTTGCCGGAAATACC  >  1/1429144‑1429523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑