Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,429,315	C→T	intergenic (‑8302/+14931)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,429,315	0	C	T	100.0%	176.4 / NA	66	intergenic (‑8302/+14931)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/37);  total (29/37)

AAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCTTGATAGTCAATTGAGATTGTCAGCTTGCGCGAATCGGGTTCAAAATCCGCCTGATAATCGGTAATTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTACTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCCGGTGAGTATGTGACTTGCCGGAAA  >  1/1429076‑1429519                                                                                                                                                                                                                                                |                                                                                                                                                                                                             aaaaaTGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGaaa                                                                                                                                                                                                   <  1:693062/251‑1 (MQ=255)             gATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTgg                                                                                                                                                                                        >  1:568297/1‑250 (MQ=255)              aTTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGc                                                                                                                                                                                       <  1:191585/250‑1 (MQ=255)                         cTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAg                                                                                                                                                                           >  1:677169/1‑251 (MQ=255)                         cTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAg                                                                                                                                                                           >  1:746853/1‑251 (MQ=255)                               gCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGt                                                                                                                                                                     >  1:872055/1‑251 (MQ=255)                               gCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGt                                                                                                                                                                     >  1:537712/1‑251 (MQ=255)                                  gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGc                                                                                                                                                                         >  1:397764/1‑244 (MQ=255)                                  gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTg                                                                                                                                                                  >  1:422224/1‑251 (MQ=255)                                     taGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                                                                                                               <  1:838375/251‑1 (MQ=255)                                        cGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCTTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACAt                                                                                                                                                             <  1:423446/250‑1 (MQ=255)                                          tGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                                                                                                           <  1:273577/250‑1 (MQ=255)                                              gTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                                                                                                               >  1:825599/1‑242 (MQ=255)                                              gTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCtgtg                                                                                                                                                       >  1:654092/1‑250 (MQ=255)                                                cTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                                                                                                           <  1:95316/244‑1 (MQ=255)                                                 tgttgtTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGAt                                                                                                                                                   <  1:399625/251‑1 (MQ=255)                                                 tgttgtTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGAt                                                                                                                                                   <  1:184280/251‑1 (MQ=255)                                                  gttgttGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATa                                                                                                                                                  >  1:296157/1‑251 (MQ=255)                                                   ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                                                                                                                 <  1:466254/251‑1 (MQ=255)                                                   ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                                                                                                                 <  1:30810/251‑1 (MQ=255)                                                   ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                                                                                                                 <  1:342967/251‑1 (MQ=255)                                                   ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGA‑TTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                                                                                                                 <  1:187175/250‑1 (MQ=255)                                                           cGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGAt                                                                                                                                         >  1:242264/1‑251 (MQ=255)                                                             tAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGAtt                                                                                                                                        >  1:463689/1‑250 (MQ=255)                                                                  gtcctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                                                                                                               <  1:601833/218‑1 (MQ=255)                                                                  gtcctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                                                                                                           <  1:30634/222‑1 (MQ=255)                                                                     ctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGt                                                                                                                               <  1:288930/250‑1 (MQ=255)                                                                        ataatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAg                                                                                                                             <  1:828515/246‑1 (MQ=255)                                                                          aatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAGACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGtt                                                                                                                           >  1:292221/3‑250 (MQ=255)                                                                          aatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                                                                                                                 >  1:173288/3‑228 (MQ=255)                                                                                      ttGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTg                                                                                                               >  1:653799/1‑250 (MQ=255)                                                                                                          ggTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGAtt                                                                                          >  1:334215/1‑251 (MQ=255)                                                                                                           gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCtgtg                                                                                                                                                       <  1:655820/189‑1 (MQ=255)                                                                                                           gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAg                                                                                                                             >  1:850259/1‑215 (MQ=255)                                                                                                           gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGAttt                                                                                         >  1:186166/1‑251 (MQ=255)                                                                                                            ttCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGt                                                                                                                               >  1:642525/1‑212 (MQ=255)                                                                                                                           atagtCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                                                                                                           >  1:491850/5‑169 (MQ=255)                                                                                                                               tCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                      <  1:707404/250‑4 (MQ=255)                                                                                                                               tCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                      <  1:636485/250‑4 (MQ=255)                                                                                                                               tCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGACCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCGATCGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCTAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                      <  1:545062/250‑4 (MQ=255)                                                                                                                                      tttttGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTc                                                              <  1:400948/251‑1 (MQ=255)                                                                                                                                                 ccttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGtt                                                   >  1:484478/16‑251 (MQ=255)                                                                                                                                                 ccttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGtt                                                   >  1:381610/16‑251 (MQ=255)                                                                                                                                                  cttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTc                                                  <  1:880160/237‑1 (MQ=255)                                                                                                                                                       gtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgtt                                             >  1:538397/10‑251 (MQ=255)                                                                                                                                                       gtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgtt                                             >  1:528246/10‑251 (MQ=255)                                                                                                                                                        tctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgt                                              <  1:301100/241‑1 (MQ=255)                                                                                                                                                        tctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtg                                               <  1:771209/240‑1 (MQ=255)                                                                                                                                                              acaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTccg                                       >  1:391640/3‑250 (MQ=255)                                                                                                                                                                        tttaaTTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGcc                            <  1:868683/247‑1 (MQ=255)                                                                                                                                                                         ttaaTTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGccc                           <  1:603440/248‑1 (MQ=255)                                                                                                                                                                                    ttCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                <  1:123428/251‑1 (MQ=255)                                                                                                                                                                                    ttCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                <  1:294071/251‑1 (MQ=255)                                                                                                                                                                                      cccAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtgtg              <  1:139580/251‑1 (MQ=255)                                                                                                                                                                                        cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTc                                                  <  1:785865/213‑1 (MQ=255)                                                                                                                                                                                        cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc            <  1:795527/251‑1 (MQ=255)                                                                                                                                                                                        cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc            <  1:217588/251‑1 (MQ=255)                                                                                                                                                                                        cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc            <  1:168851/251‑1 (MQ=255)                                                                                                                                                                                        cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATCGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc            <  1:576381/251‑1 (MQ=255)                                                                                                                                                                                                  gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                      >  1:162077/1‑180 (MQ=255)                                                                                                                                                                                                  gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                <  1:199358/237‑1 (MQ=255)                                                                                                                                                                                                  gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGACttaacgtatt  >  1:313048/1‑243 (MQ=255)                                                                                                                                                                                                  gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGCCATTGGCAAACGACCAAAGGCGGGGTTGTACATTCTGGGCGATAGCTTCAGATTCAGTTGCGTAGTTCGAACAACCTTGCCCAAACGGCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGGGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGTGTAtgtggtttaacgtctt  >  1:674304/1‑239 (MQ=255)                                                                                                                                                                                                   attgacacCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgttg                                            >  1:754933/5‑208 (MQ=255)                                                                                                                                                                                                                       gctCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc            <  1:801992/218‑1 (MQ=255)                                                                                                                                                                                                                            aGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCtg                                                                                                                                                         <  1:354475/74‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                             AAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCTTGATAGTCAATTGAGATTGTCAGCTTGCGCGAATCGGGTTCAAAATCCGCCTGATAATCGGTAATTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTACTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCCGGTGAGTATGTGACTTGCCGGAAA  >  1/1429076‑1429519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑