Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,429,300	C→T	intergenic (‑8287/+14946)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,429,300	0	C	T	100.0%	191.2 / NA	71	intergenic (‑8287/+14946)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/40);  total (31/40)

AAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCTTGATAGTCAATTGAGATTGTCAGCTTGCGCGAATCGGGTTCAAAATCCGCCTGATAATCGGTAATTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTACTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCCGGTGAGTATGTGACTTGCCGGAAA  >  1/1429054‑1429519                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            aaaTTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGc                                                                                                                                                                                                                          <  1:391621/250‑1 (MQ=255) aaaTTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCa                                                                                                                                                                                                                         >  1:453999/1‑251 (MQ=255) aaaTTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCa                                                                                                                                                                                                                         >  1:711722/1‑251 (MQ=255)           aTCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTc                                                                                                                                                                                                               <  1:500570/251‑1 (MQ=255)                 tCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTctaaatc                                                                                                                                                                                                         <  1:106774/251‑7 (MQ=255)                       aaaaaTGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGaaa                                                                                                                                                                                                   <  1:693062/251‑1 (MQ=255)                                   gATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTgg                                                                                                                                                                                        >  1:568297/1‑250 (MQ=255)                                    aTTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGc                                                                                                                                                                                       <  1:191585/250‑1 (MQ=255)                                               cTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAg                                                                                                                                                                           >  1:746853/1‑251 (MQ=255)                                               cTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAg                                                                                                                                                                           >  1:677169/1‑251 (MQ=255)                                                     gCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGt                                                                                                                                                                     >  1:537712/1‑251 (MQ=255)                                                     gCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGt                                                                                                                                                                     >  1:872055/1‑251 (MQ=255)                                                        gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGc                                                                                                                                                                         >  1:397764/1‑244 (MQ=255)                                                        gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTg                                                                                                                                                                  >  1:422224/1‑251 (MQ=255)                                                           taGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                                                                                                               <  1:838375/251‑1 (MQ=255)                                                              cGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCTTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACAt                                                                                                                                                             <  1:423446/250‑1 (MQ=255)                                                                tGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                                                                                                           <  1:273577/250‑1 (MQ=255)                                                                    gTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                                                                                                               >  1:825599/1‑242 (MQ=255)                                                                    gTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCtgtg                                                                                                                                                       >  1:654092/1‑250 (MQ=255)                                                                      cTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                                                                                                           <  1:95316/244‑1 (MQ=255)                                                                       tgttgtTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGAt                                                                                                                                                   <  1:184280/251‑1 (MQ=255)                                                                       tgttgtTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGAt                                                                                                                                                   <  1:399625/251‑1 (MQ=255)                                                                        gttgttGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATa                                                                                                                                                  >  1:296157/1‑251 (MQ=255)                                                                         ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                                                                                                                 <  1:466254/251‑1 (MQ=255)                                                                         ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                                                                                                                 <  1:342967/251‑1 (MQ=255)                                                                         ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                                                                                                                 <  1:30810/251‑1 (MQ=255)                                                                         ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGA‑TTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                                                                                                                 <  1:187175/250‑1 (MQ=255)                                                                                 cGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGAt                                                                                                                                         >  1:242264/1‑251 (MQ=255)                                                                                   tAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGAtt                                                                                                                                        >  1:463689/1‑250 (MQ=255)                                                                                        gtcctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                                                                                                               <  1:601833/218‑1 (MQ=255)                                                                                        gtcctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                                                                                                           <  1:30634/222‑1 (MQ=255)                                                                                           ctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGt                                                                                                                               <  1:288930/250‑1 (MQ=255)                                                                                              ataatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAg                                                                                                                             <  1:828515/246‑1 (MQ=255)                                                                                                aatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAGACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGtt                                                                                                                           >  1:292221/3‑250 (MQ=255)                                                                                                aatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                                                                                                                 >  1:173288/3‑228 (MQ=255)                                                                                                            ttGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTg                                                                                                               >  1:653799/1‑250 (MQ=255)                                                                                                                                ggTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGAtt                                                                                          >  1:334215/1‑251 (MQ=255)                                                                                                                                 gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCtgtg                                                                                                                                                       <  1:655820/189‑1 (MQ=255)                                                                                                                                 gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAg                                                                                                                             >  1:850259/1‑215 (MQ=255)                                                                                                                                 gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGAttt                                                                                         >  1:186166/1‑251 (MQ=255)                                                                                                                                  ttCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGt                                                                                                                               >  1:642525/1‑212 (MQ=255)                                                                                                                                                 atagtCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                                                                                                           >  1:491850/5‑169 (MQ=255)                                                                                                                                                     tCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                      <  1:636485/250‑4 (MQ=255)                                                                                                                                                     tCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                      <  1:707404/250‑4 (MQ=255)                                                                                                                                                     tCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGACCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCGATCGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCTAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                      <  1:545062/250‑4 (MQ=255)                                                                                                                                                            tttttGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTc                                                              <  1:400948/251‑1 (MQ=255)                                                                                                                                                                       ccttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGtt                                                   >  1:381610/16‑251 (MQ=255)                                                                                                                                                                       ccttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGtt                                                   >  1:484478/16‑251 (MQ=255)                                                                                                                                                                        cttcagtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTc                                                  <  1:880160/237‑1 (MQ=255)                                                                                                                                                                             gtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgtt                                             >  1:538397/10‑251 (MQ=255)                                                                                                                                                                             gtctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgtt                                             >  1:528246/10‑251 (MQ=255)                                                                                                                                                                              tctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgt                                              <  1:301100/241‑1 (MQ=255)                                                                                                                                                                              tctccaacaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtg                                               <  1:771209/240‑1 (MQ=255)                                                                                                                                                                                    acaCATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTccg                                       >  1:391640/3‑250 (MQ=255)                                                                                                                                                                                              tttaaTTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGcc                            <  1:868683/247‑1 (MQ=255)                                                                                                                                                                                               ttaaTTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGccc                           <  1:603440/248‑1 (MQ=255)                                                                                                                                                                                                          ttCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                <  1:123428/251‑1 (MQ=255)                                                                                                                                                                                                          ttCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                <  1:294071/251‑1 (MQ=255)                                                                                                                                                                                                            cccAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtgtg              <  1:139580/251‑1 (MQ=255)                                                                                                                                                                                                              cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTc                                                  <  1:785865/213‑1 (MQ=255)                                                                                                                                                                                                              cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc            <  1:795527/251‑1 (MQ=255)                                                                                                                                                                                                              cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc            <  1:168851/251‑1 (MQ=255)                                                                                                                                                                                                              cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc            <  1:217588/251‑1 (MQ=255)                                                                                                                                                                                                              cAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATCGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc            <  1:576381/251‑1 (MQ=255)                                                                                                                                                                                                                        gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTattc                                                                      >  1:162077/1‑180 (MQ=255)                                                                                                                                                                                                                        gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTAtg                <  1:199358/237‑1 (MQ=255)                                                                                                                                                                                                                        gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGACttaacgtatt  >  1:313048/1‑243 (MQ=255)                                                                                                                                                                                                                        gATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGCCATTGGCAAACGACCAAAGGCGGGGTTGTACATTCTGGGCGATAGCTTCAGATTCAGTTGCGTAGTTCGAACAACCTTGCCCAAACGGCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGGGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGTGTAtgtggtttaacgtctt  >  1:674304/1‑239 (MQ=255)                                                                                                                                                                                                                         attgacacCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCtgttg                                            >  1:754933/5‑208 (MQ=255)                                                                                                                                                                                                                                             gctCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTATTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCGGGTGAGTATGTGAc            <  1:801992/218‑1 (MQ=255)                                                                                                                                                                                                                                                  aGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCtg                                                                                                                                                         <  1:354475/74‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                            AAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCTTGATAGTCAATTGAGATTGTCAGCTTGCGCGAATCGGGTTCAAAATCCGCCTGATAATCGGTAATTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTTTTATCCAGCCGTCTTACTCTTGTTGTCATTGTGGTGTTCCTGTTGTTCCGCCGCTGTCGCCCCGGTGAGTATGTGACTTGCCGGAAA  >  1/1429054‑1429519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑