Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,429,201	A→G	intergenic (‑8188/+15045)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,429,201	0	A	G	100.0%	164.9 / NA	59	intergenic (‑8188/+15045)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/33);  total (26/33)

TGATGTTCACATGCCGCAAGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCTTGATAGTCAATTGAGATTGTCAGCTTGCGCGAATCGGGTTCAAAATCCGCCTGATAATCGGTAATTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTT  >  1/1428959‑1429432                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        tGATGTTCACATGCCGCAAGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGtgat                                                                                                                                                                                                                                 <  1:134219/251‑4 (MQ=255)                            cTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacac                                                                                                                                                                                                      <  1:699008/251‑13 (MQ=255)                            cTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacac                                                                                                                                                                                                      <  1:745130/251‑13 (MQ=255)                            cTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacac                                                                                                                                                                                                      <  1:207534/251‑13 (MQ=255)                               attaGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacacatg                                                                                                                                                                                                   <  1:550749/251‑16 (MQ=255)                                    aTTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaac                                                                                                                                                                                                        <  1:524064/241‑11 (MQ=255)                                        tCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacac                                                                                                                                                                                                      >  1:857766/1‑227 (MQ=255)                                              cccATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacac                                                                                                                                                                                                      >  1:864945/1‑221 (MQ=255)                                                 aTCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtc                                                                                                                                                                                                              <  1:302231/222‑5 (MQ=255)                                                                 gttgAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGAttg                                                                                                                                                                 >  1:236200/1‑250 (MQ=255)                                                                                  aaaaTTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTg                                                                                                                                                <  1:543249/251‑1 (MQ=255)                                                                                    aaTTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTgct                                                                                                                                              <  1:59655/251‑3 (MQ=255)                                                                                     aTTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTgctc                                                                                                                                             <  1:177733/251‑4 (MQ=255)                                                                                           gcgcGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGcc                                                                                                                                        <  1:659441/250‑1 (MQ=255)                                                                                                aaaTTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGc                                                                                                                                   <  1:391621/250‑1 (MQ=255)                                                                                                aaaTTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCa                                                                                                                                  >  1:711722/1‑251 (MQ=255)                                                                                                aaaTTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCa                                                                                                                                  >  1:453999/1‑251 (MQ=255)                                                                                                          aTCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTc                                                                                                                        <  1:500570/251‑1 (MQ=255)                                                                                                                tCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTctaaatc                                                                                                                  <  1:106774/251‑7 (MQ=255)                                                                                                                      aaaaaTGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGaaa                                                                                                            <  1:693062/251‑1 (MQ=255)                                                                                                                                  gATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTgg                                                                                                 >  1:568297/1‑250 (MQ=255)                                                                                                                                   aTTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGc                                                                                                <  1:191585/250‑1 (MQ=255)                                                                                                                                              cTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAg                                                                                    >  1:746853/1‑251 (MQ=255)                                                                                                                                              cTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAg                                                                                    >  1:677169/1‑251 (MQ=255)                                                                                                                                                    gCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGcc                                                                                                                                        <  1:692418/193‑1 (MQ=255)                                                                                                                                                    gCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGt                                                                              >  1:872055/1‑251 (MQ=255)                                                                                                                                                    gCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGt                                                                              >  1:537712/1‑251 (MQ=255)                                                                                                                                                       gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTc                                                                                                                                                                                                                      >  1:333726/1‑112 (MQ=255)                                                                                                                                                       gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaac                                                                                                                                                                                                        <  1:857539/126‑11 (MQ=255)                                                                                                                                                       gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGcc                                                                                                                                        >  1:129661/1‑190 (MQ=255)                                                                                                                                                       gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGc                                                                                  >  1:397764/1‑244 (MQ=255)                                                                                                                                                       gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTg                                                                           >  1:422224/1‑251 (MQ=255)                                                                                                                                                          taGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                        <  1:838375/251‑1 (MQ=255)                                                                                                                                                             cGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCTTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACAt                                                                      <  1:423446/250‑1 (MQ=255)                                                                                                                                                               tGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                    <  1:273577/250‑1 (MQ=255)                                                                                                                                                                   gTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                        >  1:825599/1‑242 (MQ=255)                                                                                                                                                                   gTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCtgtg                                                                >  1:654092/1‑250 (MQ=255)                                                                                                                                                                     cTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                    <  1:95316/244‑1 (MQ=255)                                                                                                                                                                      tgttgtTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGAt                                                            <  1:184280/251‑1 (MQ=255)                                                                                                                                                                      tgttgtTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGAt                                                            <  1:399625/251‑1 (MQ=255)                                                                                                                                                                       gttgttGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATa                                                           >  1:296157/1‑251 (MQ=255)                                                                                                                                                                        ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                          <  1:466254/251‑1 (MQ=255)                                                                                                                                                                        ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                          <  1:342967/251‑1 (MQ=255)                                                                                                                                                                        ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                          <  1:30810/251‑1 (MQ=255)                                                                                                                                                                        ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGA‑TTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                          <  1:187175/250‑1 (MQ=255)                                                                                                                                                                                cGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGAt                                                  >  1:242264/1‑251 (MQ=255)                                                                                                                                                                                  tAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGAtt                                                 >  1:463689/1‑250 (MQ=255)                                                                                                                                                                                       gtcctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                        <  1:601833/218‑1 (MQ=255)                                                                                                                                                                                       gtcctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                    <  1:30634/222‑1 (MQ=255)                                                                                                                                                                                          ctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGt                                        <  1:288930/250‑1 (MQ=255)                                                                                                                                                                                             ataatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAg                                      <  1:828515/246‑1 (MQ=255)                                                                                                                                                                                               aatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAGACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGtt                                    >  1:292221/3‑250 (MQ=255)                                                                                                                                                                                               aatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                          >  1:173288/3‑228 (MQ=255)                                                                                                                                                                                                           ttGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTg                        >  1:653799/1‑250 (MQ=255)                                                                                                                                                                                                                               ggTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGAtt   >  1:334215/1‑251 (MQ=255)                                                                                                                                                                                                                                gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCtgtg                                                                <  1:655820/189‑1 (MQ=255)                                                                                                                                                                                                                                gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAg                                      >  1:850259/1‑215 (MQ=255)                                                                                                                                                                                                                                gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGAttt  >  1:186166/1‑251 (MQ=255)                                                                                                                                                                                                                                 ttCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGt                                        >  1:642525/1‑212 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        TGATGTTCACATGCCGCAAGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCTTGATAGTCAATTGAGATTGTCAGCTTGCGCGAATCGGGTTCAAAATCCGCCTGATAATCGGTAATTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTT  >  1/1428959‑1429432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑