Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,429,192	C→A	intergenic (‑8179/+15054)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,429,192	0	C	A	100.0%	169.2 / NA	63	intergenic (‑8179/+15054)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/36);  total (27/36)

CGCCGTCTTGATGTTCACATGCCGCAAGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCTTGATAGTCAATTGAGATTGTCAGCTTGCGCGAATCGGGTTCAAAATCCGCCTGATAATCGGTAATTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTT  >  1/1428951‑1429432                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 cgcCGTCTTGATGTTCACATGCCGCAAGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATag                                                                                                                                                                                                                                         <  1:337650/251‑2 (MQ=255) cgcCGTCTTGATGTTCACATGCCGCAAGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATag                                                                                                                                                                                                                                         <  1:577265/251‑2 (MQ=255)     gTCTTGATGTTCACATGCCGCAAGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCTTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATagtc                                                                                                                                                                                                                                       <  1:451873/249‑4 (MQ=255)         tGATGTTCACATGCCGCAAGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGtgat                                                                                                                                                                                                                                 <  1:134219/251‑4 (MQ=255)          gATGTTCACATGCCGCAAGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATag                                                                                                                                                                                                                                         >  1:827053/1‑241 (MQ=255)                                    cTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacac                                                                                                                                                                                                      <  1:207534/251‑13 (MQ=255)                                    cTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacac                                                                                                                                                                                                      <  1:745130/251‑13 (MQ=255)                                    cTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacac                                                                                                                                                                                                      <  1:699008/251‑13 (MQ=255)                                       attaGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacacatg                                                                                                                                                                                                   <  1:550749/251‑16 (MQ=255)                                            aTTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaac                                                                                                                                                                                                        <  1:524064/241‑11 (MQ=255)                                                tCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacac                                                                                                                                                                                                      >  1:857766/1‑227 (MQ=255)                                                      cccATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaacac                                                                                                                                                                                                      >  1:864945/1‑221 (MQ=255)                                                         aTCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtc                                                                                                                                                                                                              <  1:302231/222‑5 (MQ=255)                                                                         gttgAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGAttg                                                                                                                                                                 >  1:236200/1‑250 (MQ=255)                                                                                          aaaaTTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTg                                                                                                                                                <  1:543249/251‑1 (MQ=255)                                                                                            aaTTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTgct                                                                                                                                              <  1:59655/251‑3 (MQ=255)                                                                                             aTTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTgctc                                                                                                                                             <  1:177733/251‑4 (MQ=255)                                                                                                   gcgcGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGcc                                                                                                                                        <  1:659441/250‑1 (MQ=255)                                                                                                        aaaTTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGc                                                                                                                                   <  1:391621/250‑1 (MQ=255)                                                                                                        aaaTTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCa                                                                                                                                  >  1:711722/1‑251 (MQ=255)                                                                                                        aaaTTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCa                                                                                                                                  >  1:453999/1‑251 (MQ=255)                                                                                                                  aTCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTc                                                                                                                        <  1:500570/251‑1 (MQ=255)                                                                                                                        tCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTctaaatc                                                                                                                  <  1:106774/251‑7 (MQ=255)                                                                                                                              aaaaaTGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGaaa                                                                                                            <  1:693062/251‑1 (MQ=255)                                                                                                                                          gATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTgg                                                                                                 >  1:568297/1‑250 (MQ=255)                                                                                                                                           aTTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGc                                                                                                <  1:191585/250‑1 (MQ=255)                                                                                                                                                      cTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAg                                                                                    >  1:746853/1‑251 (MQ=255)                                                                                                                                                      cTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAg                                                                                    >  1:677169/1‑251 (MQ=255)                                                                                                                                                            gCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGcc                                                                                                                                        <  1:692418/193‑1 (MQ=255)                                                                                                                                                            gCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGt                                                                              >  1:872055/1‑251 (MQ=255)                                                                                                                                                            gCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGt                                                                              >  1:537712/1‑251 (MQ=255)                                                                                                                                                               gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTc                                                                                                                                                                                                                      >  1:333726/1‑112 (MQ=255)                                                                                                                                                               gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTcagtctccaac                                                                                                                                                                                                        <  1:857539/126‑11 (MQ=255)                                                                                                                                                               gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGcc                                                                                                                                        >  1:129661/1‑190 (MQ=255)                                                                                                                                                               gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGc                                                                                  >  1:397764/1‑244 (MQ=255)                                                                                                                                                               gTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTg                                                                           >  1:422224/1‑251 (MQ=255)                                                                                                                                                                  taGCGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                        <  1:838375/251‑1 (MQ=255)                                                                                                                                                                     cGTGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCTTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACAt                                                                      <  1:423446/250‑1 (MQ=255)                                                                                                                                                                       tGCGGTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                    <  1:273577/250‑1 (MQ=255)                                                                                                                                                                           gTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                        >  1:825599/1‑242 (MQ=255)                                                                                                                                                                           gTCTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCtgtg                                                                >  1:654092/1‑250 (MQ=255)                                                                                                                                                                             cTGTTGTTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                    <  1:95316/244‑1 (MQ=255)                                                                                                                                                                              tgttgtTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGAt                                                            <  1:184280/251‑1 (MQ=255)                                                                                                                                                                              tgttgtTGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGAt                                                            <  1:399625/251‑1 (MQ=255)                                                                                                                                                                               gttgttGCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATa                                                           >  1:296157/1‑251 (MQ=255)                                                                                                                                                                                ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                          <  1:466254/251‑1 (MQ=255)                                                                                                                                                                                ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                          <  1:342967/251‑1 (MQ=255)                                                                                                                                                                                ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                          <  1:30810/251‑1 (MQ=255)                                                                                                                                                                                ttgttgCCCGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGA‑TTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                          <  1:187175/250‑1 (MQ=255)                                                                                                                                                                                        cGTAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGAt                                                  >  1:242264/1‑251 (MQ=255)                                                                                                                                                                                          tAAATGTCCTGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGAtt                                                 >  1:463689/1‑250 (MQ=255)                                                                                                                                                                                               gtcctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTAc                                                                        <  1:601833/218‑1 (MQ=255)                                                                                                                                                                                               gtcctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTc                                                                    <  1:30634/222‑1 (MQ=255)                                                                                                                                                                                                  ctGATAATCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGt                                        <  1:288930/250‑1 (MQ=255)                                                                                                                                                                                                     ataatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAg                                      <  1:828515/246‑1 (MQ=255)                                                                                                                                                                                                       aatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAGACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGtt                                    >  1:292221/3‑250 (MQ=255)                                                                                                                                                                                                       aatCAATGGATATTGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAg                                                          >  1:173288/3‑228 (MQ=255)                                                                                                                                                                                                                   ttGTCAGTTTGCGCGAATCGGGTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTg                        >  1:653799/1‑250 (MQ=255)                                                                                                                                                                                                                                       ggTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGAtt   >  1:334215/1‑251 (MQ=255)                                                                                                                                                                                                                                        gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCtgtg                                                                <  1:655820/189‑1 (MQ=255)                                                                                                                                                                                                                                        gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAg                                      >  1:850259/1‑215 (MQ=255)                                                                                                                                                                                                                                        gTTCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGAttt  >  1:186166/1‑251 (MQ=255)                                                                                                                                                                                                                                         ttCAAAATCAGCCTGATAGTCGGTGATTTTTGACACTCCTTCAGTCTCCAACACATGACGCTTAATTTTGATTTCCCAATCTGCCAGATTGACACCGCGCCCCATTTGCTCAAGCCATGGCAAGCCATGTTCTAAATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCAGTGGCGt                                        >  1:642525/1‑212 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 CGCCGTCTTGATGTTCACATGCCGCAAGTCGATCGCTTATTAGATTTTCGCCACCCATCCAAAATTGATTTTGTTGAAAAAGCCTTTAAAAAATTAGGGCGCGAAATTAATTTATCGGTTCATTAAAAAATGCGGTCGATTTGACCGCACTTTAAGCTGTATAGCGTGCGGTCTGTTGTTGCCCGTAAATGTCTTGATAGTCAATTGAGATTGTCAGCTTGCGCGAATCGGGTTCAAAATCCGCCTGATAATCGGTAATTTTTGACACTCCTTCCGTTCCCAGTACATGCCGCTTTATTTTAATTTCCCAATCCGCCAGATTCACACCGCGCCCCATTTGTTCAAGCCACGGCAAGCCATGTTCCAGATCTAGAAACCAGTCATTGGCAAACGACCAAAGGCGGGTTTGTACATTCTGTGCGATAGCTTCAGATTCGGTGGCGTAGTTCGAAAAACCTTGCCCAAACGTCCAATCGTGATTT  >  1/1428951‑1429432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑