Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,428,722	C→T	intergenic (‑7709/+15524)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,428,722	0	C	T	100.0%	165.3 / NA	61	intergenic (‑7709/+15524)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/28);  total (33/28)

CAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGACATTCCCGAAATGGCAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAGTATATGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGAAATGCTCGCACAAGGTGTGCGAAAAGCTGAAATGGCGCG  >  1/1428478‑1428952                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       cAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTTCCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTccc                                                                                                                                                                                                                                  <  1:603724/251‑1 (MQ=255)             gAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATgg                                                                                                                                                                                                                           >  1:727570/1‑246 (MQ=255)                 tCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAgtgt                                                                                                                                                                                                                    <  1:629/249‑1 (MQ=255)                    tCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTg                                                                                                                                                                                                               <  1:521402/251‑1 (MQ=255)                              aCGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGCAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGa                                                                                                                                                                                                     >  1:423024/1‑251 (MQ=255)                              aCGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGCACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGa                                                                                                                                                                                                     >  1:523086/1‑251 (MQ=255)                                  tGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAgc                                                                                                                                                                                                 <  1:30772/251‑1 (MQ=255)                                         ctGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAgg                                                                                                                                                                                                                 <  1:703086/227‑1 (MQ=255)                                                aCTGTCTTACCATACCATGGTAGTAAAGAAATCGGTACCGGATTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCGCCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTGCTTGAAGc                                                                                                                                                                                   <  1:561371/251‑1 (MQ=255)                                                 cTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCg                                                                                                                                                                                  >  1:477110/1‑251 (MQ=255)                                                   gTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGga                                                                                                                                                                                >  1:212146/1‑251 (MQ=255)                                                   gTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATACCCGAAATGGTAAgtgt                                                                                                                                                                                                                    <  1:284718/215‑1 (MQ=255)                                                         cccTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGaa                                                                                                                                                                                                    >  1:712291/1‑225 (MQ=255)                                                             aCCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGat                                                                                                                                                                                                          >  1:601015/1‑215 (MQ=255)                                                             aCCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGc                                                                                                                                                                                   >  1:637412/1‑238 (MQ=255)                                                              ccATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAg                                                                                                                                                                     >  1:548158/1‑251 (MQ=255)                                                                 tGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTg                                                                                                                                                                                                               <  1:854654/206‑1 (MQ=255)                                                                   gtagtaAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAAc                                                                                                                                                                 <  1:736336/250‑1 (MQ=255)                                                                   gtagtaAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAAc                                                                                                                                                                 >  1:266613/1‑250 (MQ=255)                                                                   gtagtaAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAAc                                                                                                                                                                 >  1:14732/1‑250 (MQ=255)                                                                                 ggTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGat                                                                                                                                                                                                          <  1:199897/195‑1 (MQ=255)                                                                                  gTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGat                                                                                                                                                                                                          >  1:152078/1‑194 (MQ=255)                                                                                           tAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTgatgat                                                                                                                                        <  1:640079/251‑1 (MQ=255)                                                                                                         aaaaaaGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTcc                                                                                                                          <  1:261624/251‑1 (MQ=255)                                                                                                         aaaaaaGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTcc                                                                                                                          <  1:291737/251‑1 (MQ=255)                                                                                                                acttaaacttaaAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGc                                                                                                                     <  1:78212/249‑1 (MQ=255)                                                                                                                    aaacttaaaATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAagcc                                                                                                               <  1:301568/251‑1 (MQ=255)                                                                                                                      acttaaaATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAagccag                                                                                                             >  1:203029/1‑251 (MQ=255)                                                                                                                       cttaaaATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGc                                                                                                                                                                                   <  1:155296/180‑1 (MQ=255)                                                                                                                       cttaaaATAAGTCTTTCGCCTCTGAAAA‑GGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAagccagc                                                                                                            >  1:253186/1‑250 (MQ=255)                                                                                                                                 gTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGa                                                                                                     <  1:393093/248‑1 (MQ=255)                                                                                                                                   cTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGc                                                                                                <  1:581274/251‑1 (MQ=255)                                                                                                                                         ccTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGTGGATAAACGCCAAATTCCAATCCCAAGCCACCCAAAAGATGGCCAAtata                                                                                          >  1:715248/1‑251 (MQ=255)                                                                                                                                         ccTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAAtata                                                                                          >  1:746871/1‑251 (MQ=255)                                                                                                                                          ctctGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAAtatac                                                                                         <  1:662233/251‑2 (MQ=255)                                                                                                                                              gAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTc                                                                                                                           <  1:885474/213‑1 (MQ=255)                                                                                                                                                                    gATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCa                                                                >  1:627571/1‑250 (MQ=255)                                                                                                                                                                            gagaAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAg                                                         <  1:174844/249‑1 (MQ=255)                                                                                                                                                                               aaaCCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGtctt                                                     >  1:313335/1‑250 (MQ=255)                                                                                                                                                                                    aTGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGt                                                                                      <  1:493451/212‑1 (MQ=255)                                                                                                                                                                                    aTGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTcc                                                                            >  1:760670/1‑222 (MQ=255)                                                                                                                                                                                    aTGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACt                                               <  1:872266/251‑1 (MQ=255)                                                                                                                                                                                     tGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTc                                              <  1:615092/251‑1 (MQ=255)                                                                                                                                                                                     tGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTc                                              <  1:511924/251‑1 (MQ=255)                                                                                                                                                                                     tGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTc                                              <  1:84541/251‑1 (MQ=255)                                                                                                                                                                                         aCGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAg                                                                                                      <  1:594050/191‑1 (MQ=255)                                                                                                                                                                                         aCGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCtt                                                                          <  1:392324/219‑1 (MQ=255)                                                                                                                                                                                              aCCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAAtatac                                                                                         >  1:777079/1‑198 (MQ=255)                                                                                                                                                                                              aCCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGAAATg                                     >  1:396053/1‑251 (MQ=255)                                                                                                                                                                                                   gTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAgttg                                                                                                                                                                                                               >  1:164594/1‑73 (MQ=255)                                                                                                                                                                                                   gTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGAAATGCTCg                                 >  1:246761/1‑250 (MQ=255)                                                                                                                                                                                                      gAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGaaa                                       >  1:676605/1‑241 (MQ=255)                                                                                                                                                                                                               ccGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGAAATGCTCGCACAAGGTGTGCg                    >  1:777076/1‑251 (MQ=255)                                                                                                                                                                                                                 ggATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGAAATGCTCGCACAAGGTGTGCGaa                  >  1:298645/1‑251 (MQ=255)                                                                                                                                                                                                                  gatgatAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGtcttc                                                    >  1:804038/1‑216 (MQ=255)                                                                                                                                                                                                                          cGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGAAATGCTCGCACAAGGTGTGCGaa                  >  1:199088/1‑242 (MQ=255)                                                                                                                                                                                                                          cGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGAAATGCTCGCACAAGGTGTGCGAAAAGCTGaaa         >  1:598579/1‑251 (MQ=255)                                                                                                                                                                                                                                 ttCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGAAATGCTCGCACAAGGTGTGCGAAAAGCTGAAATGgcgcg  >  1:392859/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGc                                                                                                                                                                                   >  1:893719/1‑72 (MQ=255)                                                                                                                                                                                                                                   ccTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGAAATGCTCGCACAAgg                          >  1:614403/1‑225 (MQ=255)                                                                                                                                                                                                                                             tGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAATATACGGt                                                                                      >  1:364938/1‑155 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       CAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGACATTCCCGAAATGGCAAGTGTAGGTGAAGATATTGAAAGCGCACTACTTGAAGCGGAAGAAGGTTTAGAAACCGCCCTTGAATTTTATTTTGATGATAAACGCCCAATTCCAATGCCAAGCCAGCCAAAAGATGGGCAGTATATGGTTCCGCTTTCCTTATTGCGTTCACTAAAAGTCTTCTTACTCAATGAAATGCTCGCACAAGGTGTGCGAAAAGCTGAAATGGCGCG  >  1/1428478‑1428952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑