Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,428,518	G→C	intergenic (‑7505/+15728)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,428,518	0	G	C	100.0%	146.6 / NA	53	intergenic (‑7505/+15728)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (30/23);  total (30/23)

GTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGACATTCCCGAAATGGCAAGTGTAGGTGAAGATATTGAAAGC  >  1/1428297‑1428761                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    gTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAACTCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGgtag                                                                                                                                                                                                                        >  1:35867/1‑251 (MQ=255)                                                           caaAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATaa                                                                                                                                                              >  1:180132/1‑251 (MQ=255)                                                              aaaTGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTc                                                                                                                                                           <  1:211390/251‑1 (MQ=255)                                                                          tataacaaATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAAc                                                                                                                                                                                                                                             <  1:89479/154‑1 (MQ=255)                                                                                      gttgtATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGttt                                                                                                                                   >  1:53635/1‑251 (MQ=255)                                                                                      gttgtATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGttt                                                                                                                                   >  1:416876/1‑251 (MQ=255)                                                                                                    tCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAgggg                                                                                                                                        >  1:747215/1‑232 (MQ=255)                                                                                                    tCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGGGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTa                                                                                                                     >  1:375842/1‑251 (MQ=255)                                                                                                       cTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAgg                                                                                                                  >  1:586152/1‑251 (MQ=255)                                                                                                       cTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAgg                                                                                                                  >  1:687252/1‑251 (MQ=255)                                                                                                       cTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAg                                                                                                                   >  1:252977/1‑250 (MQ=255)                                                                                                         gTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTc                                                                                                                                                                                              >  1:381336/1‑173 (MQ=255)                                                                                                         gTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGaaa                                                                                                                                             <  1:748255/222‑1 (MQ=255)                                                                                                         gTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGgag                                                                                                                >  1:218039/1‑251 (MQ=255)                                                                                                         gTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGgag                                                                                                                >  1:241349/1‑251 (MQ=255)                                                                                                         gTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGgag                                                                                                                >  1:398867/1‑251 (MQ=255)                                                                                                         gTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGgag                                                                                                                >  1:162364/1‑251 (MQ=255)                                                                                                              gCAAAGGAGGTACCCCGATGAAATGCAGTGAATTCTTGCGATGCTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGgt                                                                                                                                                                                                                          <  1:877078/140‑1 (MQ=255)                                                                                                              gCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGc                                                                                                                                                     <  1:307433/209‑1 (MQ=255)                                                                                                              gCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTAACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAgggg                                                                                                                                        >  1:71654/1‑222 (MQ=255)                                                                                                                            ccGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTAccc                                                                                             <  1:463893/251‑1 (MQ=255)                                                                                                                              gATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGaaa                                                                                                                                             <  1:287575/201‑1 (MQ=255)                                                                                                                              gATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGaaa                                                                                                                                             >  1:17369/1‑201 (MQ=255)                                                                                                                              gATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTAcc                                                                                              >  1:748841/1‑248 (MQ=255)                                                                                                                               aTGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAg                                                                                                                                                                                                                   >  1:645345/1‑130 (MQ=255)                                                                                                                               aTGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTAcc                                                                                              >  1:614635/1‑247 (MQ=255)                                                                                                                               aTGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTAcc                                                                                              >  1:30586/1‑247 (MQ=255)                                                                                                                               aTGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTAcc                                                                                              >  1:420798/1‑247 (MQ=255)                                                                                                                                   aaTACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAAt                                                                                                                                                                >  1:561268/1‑177 (MQ=255)                                                                                                                                           gAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGcc                                                                                                                                                    >  1:324328/1‑181 (MQ=255)                                                                                                                                                      aTACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGacttaaac                                                                                                                                                                       <  1:566944/151‑1 (MQ=255)                                                                                                                                                      aTACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAAc                                                                    <  1:886916/250‑1 (MQ=255)                                                                                                                                                          ttgcttgAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTAc                                                               <  1:9201/251‑1 (MQ=255)                                                                                                                                                          ttgcttgAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTAc                                                               <  1:128339/251‑1 (MQ=255)                                                                                                                                                            gcttgAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTg                                                                                        <  1:552088/224‑1 (MQ=255)                                                                                                                                                               tgAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTTCGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTcc                                                          <  1:112105/251‑1 (MQ=255)                                                                                                                                                               tgAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTcc                                                          <  1:175707/251‑1 (MQ=255)                                                                                                                                                               tgAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTcc                                                          <  1:192411/251‑1 (MQ=255)                                                                                                                                                                   cAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTg                                                       <  1:588059/250‑1 (MQ=255)                                                                                                                                                                    aaGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTc                                                     <  1:14726/251‑1 (MQ=255)                                                                                                                                                                     aGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCt                                                         <  1:204947/246‑1 (MQ=255)                                                                                                                                                                     aGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCa                                                    >  1:23301/1‑251 (MQ=255)                                                                                                                                                                       gCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCa                                                    >  1:593652/1‑249 (MQ=255)                                                                                                                                                                               gTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTg                                                       >  1:376303/1‑238 (MQ=255)                                                                                                                                                                                      cAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTTCCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTccc                                   <  1:603724/251‑1 (MQ=255)                                                                                                                                                                                             gcgcGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGcc                                                                                                                                                    <  1:471204/131‑1 (MQ=255)                                                                                                                                                                                                  gAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATgg                            >  1:727570/1‑246 (MQ=255)                                                                                                                                                                                                      tCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAgtgt                     <  1:629/249‑1 (MQ=255)                                                                                                                                                                                                         tCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTg                <  1:521402/251‑1 (MQ=255)                                                                                                                                                                                                                  aaCGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTa                                                                >  1:457126/1‑194 (MQ=255)                                                                                                                                                                                                                   aCGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGCAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGa      >  1:423024/1‑251 (MQ=255)                                                                                                                                                                                                                   aCGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGCACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGa      >  1:523086/1‑251 (MQ=255)                                                                                                                                                                                                                       tGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGATATTCCCGAAATGGTAAGTGTAGGTGAAGATATTGAAAgc  <  1:30772/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                    GTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTTTCTCAAACGATTTAAGGAGAAACCATGTTACGCTACCCTGTTGAAATCACCCCGGATGATAACGGTACATTCCTTGTCACTTGCCCCGACATTCCCGAAATGGCAAGTGTAGGTGAAGATATTGAAAGC  >  1/1428297‑1428761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑