Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,428,395	C→T	intergenic (‑7382/+15851)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,428,395	0	C	T	100.0%	25.0 / NA	11	intergenic (‑7382/+15851)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/6);  total (5/6)

ATATGTTCCTTAGTAAAAAATAGAAACGCCCATTCTACAAAACCCAAGACATTTCCGTAAGTTCTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTT  >  1/1428171‑1428632                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              atatGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTAccc                                                                                                                                                                                                                      <  1:671737/250‑1 (MQ=255)                    aTAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGc                                                                                                                                                                                                                                   <  1:120873/218‑1 (MQ=255)                    aTAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTc                                                                                                                                                                                                  <  1:106857/251‑1 (MQ=255)                                                                                                                          ccTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATg                                                                                                                      >  1:72305/1‑225 (MQ=255)                                                                                                                               gTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAACTCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGgtag                                                                                       >  1:35867/1‑251 (MQ=255)                                                                                                                                                                                         caaAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATaa                             >  1:180132/1‑251 (MQ=255)                                                                                                                                                                                            aaaTGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTc                          <  1:211390/251‑1 (MQ=255)                                                                                                                                                                                                        tataacaaATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAAc                                                                                                            <  1:89479/154‑1 (MQ=255)                                                                                                                                                                                                                    gttgtATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGt                                                                                                                                 <  1:37601/124‑1 (MQ=255)                                                                                                                                                                                                                    gttgtATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGttt  >  1:416876/1‑251 (MQ=255)                                                                                                                                                                                                                    gttgtATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGttt  >  1:53635/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              ATATGTTCCTTAGTAAAAAATAGAAACGCCCATTCTACAAAACCCAAGACATTTCCGTAAGTTCTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTCTTTCGCCTCTGAAAAGGGGCGTTT  >  1/1428171‑1428632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑