Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,428,372	C→T	intergenic (‑7359/+15874)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,428,372	0	C	T	100.0%	24.6 / NA	11	intergenic (‑7359/+15874)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

ATAACGATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACGCCCATTCTACAAAACCCAAGACATTTCCGTAAGTTCTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTC  >  1/1428131‑1428608                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             atgacaaTAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAAttt                                                                                                                                                                                                                                     >  1:439048/7‑251 (MQ=255)      aaTAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTtgttgt                                                                                                                                                                                                                                <  1:355306/250‑1 (MQ=255)                     aatGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACaaa                                                                                                                                                                                                                                        <  1:389025/228‑1 (MQ=255)                                         atatGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTAccc                                                                                                                                                                                              <  1:671737/250‑1 (MQ=255)                                                            aTAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGc                                                                                                                                                                                                           <  1:120873/218‑1 (MQ=255)                                                            aTAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTc                                                                                                                                                                          <  1:106857/251‑1 (MQ=255)                                                                                  aCCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATct                                                                                                                                                                                                                       <  1:568196/184‑2 (MQ=255)                                                                                                                                                                  ccTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATg                                                                                              >  1:72305/1‑225 (MQ=255)                                                                                                                                                                       gTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAACTCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGgtag                                                               >  1:35867/1‑251 (MQ=255)                                                                                                                                                                                                                                 caaAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATaa     >  1:180132/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaaTGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTc  <  1:211390/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                             ATAACGATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACGCCCATTCTACAAAACCCAAGACATTTCCGTAAGTTCTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTC  >  1/1428131‑1428608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑