Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,428,367	T→A	intergenic (‑7354/+15879)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,428,367	0	T	A	100.0%	27.7 / NA	12	intergenic (‑7354/+15879)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (4/8);  total (4/8)

AAATTAAAATAACGATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACGCCCATTCTACAAAACCCAAGACATTTCCGTAAGTTCTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTC  >  1/1428123‑1428608                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  aaattaaaatGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATata                                                                                                                                                                                                                                             <  1:465795/251‑3 (MQ=255)         atgacaaTAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAAttt                                                                                                                                                                                                                                     >  1:439048/7‑251 (MQ=255)              aaTAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTtgttgt                                                                                                                                                                                                                                <  1:355306/250‑1 (MQ=255)                             aatGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACaaa                                                                                                                                                                                                                                        <  1:389025/228‑1 (MQ=255)                                                 atatGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTAccc                                                                                                                                                                                              <  1:671737/250‑1 (MQ=255)                                                                    aTAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGc                                                                                                                                                                                                           <  1:120873/218‑1 (MQ=255)                                                                    aTAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTc                                                                                                                                                                          <  1:106857/251‑1 (MQ=255)                                                                                          aCCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATct                                                                                                                                                                                                                       <  1:568196/184‑2 (MQ=255)                                                                                                                                                                          ccTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATg                                                                                              >  1:72305/1‑225 (MQ=255)                                                                                                                                                                               gTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAACTCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGgtag                                                               >  1:35867/1‑251 (MQ=255)                                                                                                                                                                                                                                         caaAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATaa     >  1:180132/1‑251 (MQ=255)                                                                                                                                                                                                                                            aaaTGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTc  <  1:211390/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  AAATTAAAATAACGATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACGCCCATTCTACAAAACCCAAGACATTTCCGTAAGTTCTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAGTAAAGAAATCGGTACCGGCTTAGTTCACAAAATCAAAAAAGACTTAAACTTAAAATAAGTC  >  1/1428123‑1428608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑