Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,428,341	C→T	intergenic (‑7328/+15905)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,428,341	0	C	T	100.0%	39.8 / NA	17	intergenic (‑7328/+15905)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/13);  total (4/13)

AATCAATGCAAAAGGGCTGCATAAAATTAAAATAACGATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACGCCCATTCTACAAAACCCAAGACATTTCCGTAAGTTCTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAG  >  1/1428100‑1428547                                                                                                                                                                                                                                                  |                                                                                                                                                                                                               aaTCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGGTTATCAAATAAATCATTATTTAAAAAac                                                                                                                                                                                                       <  1:342690/251‑1 (MQ=255)      aTGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACacaa                                                                                                                                                                                                   >  1:665719/1‑250 (MQ=255)          aaaaGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACaaaa                                                                                                                                                                                              <  1:765060/251‑1 (MQ=255)           aaaGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACaaaaa                                                                                                                                                                                             <  1:717576/251‑1 (MQ=255)                   gCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATCTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACaaaaa                                                                                                                                                                                             <  1:690152/243‑1 (MQ=255)                    cATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGcac                                                                                                                                                                                     <  1:86253/250‑3 (MQ=255)                        aaattaaaatGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATata                                                                                                                                                                                <  1:465795/251‑3 (MQ=255)                                atgacaaTAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAAttt                                                                                                                                                                        >  1:439048/7‑251 (MQ=255)                                     aaTAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTtgttgt                                                                                                                                                                   <  1:355306/250‑1 (MQ=255)                                                    aatGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGt                                                                                                                                                                                          <  1:340025/213‑1 (MQ=255)                                                    aatGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACaaa                                                                                                                                                                           <  1:389025/228‑1 (MQ=255)                                                                        atatGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTAccc                                                                                                                                 <  1:671737/250‑1 (MQ=255)                                                                                           aTAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGc                                                                                                                                              <  1:120873/218‑1 (MQ=255)                                                                                           aTAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTc                                                                                                             <  1:106857/251‑1 (MQ=255)                                                                                                                 aCCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATct                                                                                                                                                          <  1:568196/184‑2 (MQ=255)                                                                                                                                                                                                 ccTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATg                                 >  1:72305/1‑225 (MQ=255)                                                                                                                                                                                                      gTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAACTCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGACTGCCAAACTGTCTTTCCCTACCATGgtag  >  1:35867/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                               AATCAATGCAAAAGGGCTGCATAAAATTAAAATAACGATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACGCCCATTCTACAAAACCCAAGACATTTCCGTAAGTTCTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTCTTGCGATACTTGCTTGAGCAAGGCTGCAAAGTCGAAGCAACCAAGCGCGGAAGTCATCGCAAGGTAACGTTGAATGAGTGCCAAACTGTCTTTCCCTACCATGGTAG  >  1/1428100‑1428547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑