Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,428,201	C→T	intergenic (‑7188/+16045)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,428,201	0	C	T	100.0%	72.8 / NA	29	intergenic (‑7188/+16045)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/18);  total (11/18)

ATACCGATTTTAAATACGTTTTTTGCCCTCTACGCTGTTTGATTTTCTTGACTATCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATAACGATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACGCCCATTCTACAAAACCCAAGACATTTCCGTAAGTTCTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTC  >  1/1428039‑1428440                                                                                                                                                                   |                                                                                                                                                                                                                                                cttgataatcatttaatgccttttGCCCTCTACGCTGTTTGATTTTTTTCACTATCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTaaaaa                                                                                                                                                         >  1:900277/21‑251 (MQ=255)          catttaatgccttttGCCCTCTACGCTGTTTGATTTTTTTCACTATCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAg                                                                                                                                                                         <  1:458755/215‑1 (MQ=255)                          cccTCTACGCTGTTTGATTTTTTTCACTATCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTg                                                                                                                                >  1:569146/1‑251 (MQ=255)                             tctACGCTGTTTGATTTTTTTCACTATCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGaaa                                                                                                                             <  1:9315/251‑1 (MQ=255)                              ctACGCTGTTTGATTTTTTTCACTATCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGaaaa                                                                                                                            >  1:467685/1‑251 (MQ=255)                                     gTTTGATTTTTTTCACTATCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGCTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGa                                                                                                                               >  1:756041/1‑241 (MQ=255)                                                 tcaCTATCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAatca                                                                                                         >  1:837702/3‑251 (MQ=255)                                                      aTCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTg                                                                                                                                <  1:213759/223‑1 (MQ=255)                                                      aTCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTCCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCt                                                                                                                                    >  1:408488/1‑219 (MQ=255)                                                              aaTCAATGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGGTTATCAAATAAATCATTATTTAAAAAac                                                                                            <  1:342690/251‑1 (MQ=255)                                                                   aTGCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACacaa                                                                                        >  1:665719/1‑250 (MQ=255)                                                                     gCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCattat                                                                                                                                                               >  1:69616/1‑177 (MQ=255)                                                                     gCAAAAGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGt                                                                                                                                                >  1:73813/1‑192 (MQ=255)                                                                       aaaaGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACaaaa                                                                                   <  1:765060/251‑1 (MQ=255)                                                                        aaaGGGCTGCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACaaaaa                                                                                  <  1:717576/251‑1 (MQ=255)                                                                                gCATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATCTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACaaaaa                                                                                  <  1:690152/243‑1 (MQ=255)                                                                                 cATAAAATTAAAATGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGcac                                                                          <  1:86253/250‑3 (MQ=255)                                                                                     aaattaaaatGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATata                                                                     <  1:465795/251‑3 (MQ=255)                                                                                       attaaaatGACAATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCattat                                                                                                                                                               <  1:644845/159‑1 (MQ=255)                                                                                             atgacaaTAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAAttt                                                             >  1:439048/7‑251 (MQ=255)                                                                                                  aaTAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTtgttgt                                                        <  1:355306/250‑1 (MQ=255)                                                                                                             cAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAatc                                                                                                          >  1:471460/1‑190 (MQ=255)                                                                                                                 aatGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGt                                                                               <  1:340025/213‑1 (MQ=255)                                                                                                                 aatGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACaaa                                                                <  1:389025/228‑1 (MQ=255)                                                                                                                                    gATATGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAg                                                                                                                                                                         <  1:210695/104‑1 (MQ=255)                                                                                                                                     atatGTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTAccc                      <  1:671737/250‑1 (MQ=255)                                                                                                                                         gTTCCTTAGTAAAAAATAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCatt                                                                                                                                                                 <  1:436880/107‑1 (MQ=255)                                                                                                                                                        aTAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGc                                   <  1:120873/218‑1 (MQ=255)                                                                                                                                                        aTAGAAACTCCTATTTTACGAAACCCAAGACATTTCCGTAAGTTTTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATTATTTAAAAAACACAACAAAAATGTTGCACATATAACAAATTTGTTGTATTCTATCTTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTc  <  1:106857/251‑1 (MQ=255)                                                                                                                                                                   |                                                                                                                                                                                                                                                ATACCGATTTTAAATACGTTTTTTGCCCTCTACGCTGTTTGATTTTCTTGACTATCCAGTAAATCAATGCAAAAGGGCTGCATAAAATTAAAATAACGATAATTCCGGCAATAATGTTGGTTGTGTTCATAGATATGTTCCTTAGTAAAAAATAGAAACGCCCATTCTACAAAACCCAAGACATTTCCGTAAGTTCTCCCTCAAATTTATTTCAATTTTGCGATCTGCGTCGCAGATTCCATTATTAAAAAAACCTACGTTTTAAACAGGCTTTTGAAAAAAGTTTATCAAATAAATCATCACTTAAAAAACACAACAAAAATGTTGCTCATACAACAAATTTGTTGTATTCTATCCTCACTGTATAGCAAAGGAGGTACCCCGATGAAATACAGTGAATTC  >  1/1428039‑1428440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑