Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,425,545	C→T	intergenic (‑4532/+18701)	unknown ← / ← unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,425,545	0	C	T	100.0%	154.1 / NA	57	intergenic (‑4532/+18701)	unknown/unknown	tRNA‑Met‑CAT/tRNA‑Gln‑TTG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/27);  total (30/27)

AATCAACGGTAGACGCGAAACATTAACTATCGGTAAATACGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCTGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTACGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCAAAAAGCGCGAACGCGTTCTAACCCCG  >  1/1425299‑1425791                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aaTCAACGGTAGACGCGAAACATTAACTCTCGGTAAATACGGCGCCGACGAAATAACTCTCGCAGAAGCGCGCGAAAAATTAATGACCGCAAGGAAGCGGTTCAGTGAAGACTTTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTatgaac                                                                                                                                                                                                                                                    <  1:487766/251‑1 (MQ=255) aaTCAACGGTAGACGCGAAACATTAACTATCGGTAAATACGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTatgaac                                                                                                                                                                                                                                                    <  1:829285/251‑1 (MQ=255) aaTCAACGGTAGACGCAAACCATTAACTATCGGTAAATACGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTatgaac                                                                                                                                                                                                                                                    <  1:287486/251‑1 (MQ=255)  aTCAACGGTAGACGCGAAACATTAACTATCGGTAAATCCGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTatgaacg                                                                                                                                                                                                                                                   <  1:701147/251‑1 (MQ=255)  aTCAACGGTAGACGCGAAACATTAACTATCGGTAAATACGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTatgaac                                                                                                                                                                                                                                                    <  1:316508/250‑1 (MQ=255)      aCGGTAGACGCGAAACATTAACTATCGGTAAATACGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGat                                                                                                                                                                                                                                               >  1:281010/1‑251 (MQ=255)             aCGCGAAACATTAACTATCGGTAAATCCGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTaa                                                                                                                                                                                                                                          <  1:401494/249‑1 (MQ=255)                         aaCTATCGGAAAATACGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGa                                                                                                                                                                                                                            <  1:492969/251‑1 (MQ=255)                           cTATCGGTAAATCCGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGAAAGAAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAg                                                                                                                                                                                                                                        <  1:65576/237‑1 (MQ=255)                             aTCGGTAAATACGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTaa                                                                                                                                                                                                                                          >  1:779187/1‑233 (MQ=255)                                      tACGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGAc                                                                                                                                                                                                               <  1:538499/251‑1 (MQ=255)                                               gACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGTCCGAAATCa                                                                                                                                                                                                       >  1:509733/1‑250 (MQ=255)                                                    aaTAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACgg                                                                                                                                                                                                 >  1:856667/1‑251 (MQ=255)                                                          tctcGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTaa                                                                                                                                                                                                                                          >  1:137041/1‑204 (MQ=255)                                                            tcGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGat                                                                                                                                                                                                                                               >  1:599225/1‑197 (MQ=255)                                                             cGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATc                                                                                                                                                                                         >  1:620379/1‑250 (MQ=255)                                                                          aaaaaaTTAATATTCACAAGGAAGCAGGTCAGGGAAGGCTTTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCaa                                                                                                                                                                           <  1:396583/250‑1 (MQ=255)                                                                            aaaaTTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATa                                                                                                                                                                         <  1:801969/251‑1 (MQ=255)                                                                                       ccgaaaGGAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGAt                                                                                                                                                              <  1:116833/247‑1 (MQ=255)                                                                                        agaaagaaaGCAGGTCGGTGAAGGCATTTCCCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGAt                                                                                                                                                              <  1:460723/243‑1 (MQ=255)                                                                                             ggAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAAt                                                                                                                                                                     <  1:478846/238‑1 (MQ=255)                                                                                              gAAGCAGGTCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTg                                                                                                                                                       >  1:34005/1‑251 (MQ=255)                                                                                                     gTCAGGGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCt                                                                                                                                                  <  1:689406/249‑1 (MQ=255)                                                                                                      tCAGTGAAGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTcc                                                                                                                                               <  1:390824/251‑1 (MQ=255)                                                                                                             aGGCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCa                                                                                                                                        >  1:394821/1‑251 (MQ=255)                                                                                                              ggCATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGa                                                                                                                                                                                                                            >  1:57942/1‑166 (MQ=255)                                                                                                                cATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTAc                                                                                                                                                                                                   >  1:238452/1‑189 (MQ=255)                                                                                                                cATTTCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCAttt                                                                                                                                     >  1:727993/1‑251 (MQ=255)                                                                                                                 ttttCTCCCGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATGCCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCAtttt                                                                                                                                    <  1:41647/250‑1 (MQ=255)                                                                                                                                          aTCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAg                                                                                                                                                                <  1:82385/198‑1 (MQ=255)                                                                                                                                            cAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTa                                                                                                         >  1:45612/1‑251 (MQ=255)                                                                                                                                               aaaGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATGCCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCg                                                                                                      <  1:99927/251‑1 (MQ=255)                                                                                                                                                    ccTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATg                                                                                                 >  1:440842/1‑251 (MQ=255)                                                                                                                                                          ccGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGAtt                                                                                            >  1:655163/1‑250 (MQ=255)                                                                                                                                                            gCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTAc                                                                                                        >  1:512179/1‑236 (MQ=255)                                                                                                                                                                 gCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCa                                                                                                                                              <  1:849756/193‑1 (MQ=255)                                                                                                                                                                    gATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAAt                                                                                                                     >  1:245183/1‑215 (MQ=255)                                                                                                                                                                         cTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAAt                                                                                                                                                                                                                          <  1:298622/109‑1 (MQ=255)                                                                                                                                                                         cTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAAc                                                                            <  1:667450/251‑1 (MQ=255)                                                                                                                                                                                         gAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGa                                                            >  1:517811/1‑251 (MQ=255)                                                                                                                                                                                         gAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGa                                                            >  1:307631/1‑251 (MQ=255)                                                                                                                                                                                                aCCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTg                                                                                                                                                       >  1:857062/1‑153 (MQ=255)                                                                                                                                                                                                 ccTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTg                                                                                                                                                       <  1:447102/152‑1 (MQ=255)                                                                                                                                                                                                 ccTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGc                                                                                                <  1:771401/207‑1 (MQ=255)                                                                                                                                                                                                  cTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGa                                                                                                                                                               >  1:275275/1‑143 (MQ=255)                                                                                                                                                                                                                   ggCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCaa                                  >  1:506311/1‑251 (MQ=255)                                                                                                                                                                                                                    gCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCaa                                  <  1:75234/250‑1 (MQ=255)                                                                                                                                                                                                                           gTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCaaa                          >  1:397955/1‑251 (MQ=255)                                                                                                                                                                                                                           gTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCaaa                          >  1:594267/1‑251 (MQ=255)                                                                                                                                                                                                                           gTACAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCaaa                          >  1:9928/1‑251 (MQ=255)                                                                                                                                                                                                                              cAAAAGCCCTCCGCGTGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCAAAAAg                       >  1:857433/1‑251 (MQ=255)                                                                                                                                                                                                                                             tGGCCACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCAAAAAGCGCGAACGCGTTCTa        <  1:828198/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCAAAAAGCGCGAACGCGTTCTAAccc    <  1:697301/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cACTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCAAAAAGCGCGAACGCGTTCTAAccc    <  1:378174/251‑1 (MQ=255)                                                                                                                                                                                                                                                   cTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGAt                                                                                                                                                              <  1:700777/95‑1 (MQ=255)                                                                                                                                                                                                                                                   cTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCt                                                                                                          <  1:137524/147‑1 (MQ=255)                                                                                                                                                                                                                                                   cTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTc                                                                                           <  1:590971/162‑1 (MQ=255)                                                                                                                                                                                                                                                   cTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCg                                                             >  1:799321/1‑192 (MQ=255)                                                                                                                                                                                                                                                   cTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTg                                     <  1:731675/216‑1 (MQ=255)                                                                                                                                                                                                                                                   cTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCAAAAAGCGCGAACGCGTTCTAACCCCg  >  1:578886/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCAAAAAGCGCGAACGCGTTCTAACCCCg  >  1:645151/1‑251 (MQ=255)                                                                                                                                                                                                                                                   cTTATGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCAAAAAGCGCGAACGCGTTCTAACCCCg  >  1:539611/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       AATCAACGGTAGACGCGAAACATTAACTATCGGTAAATACGGCGCCGACGGAATAAATCTCGCAGAAGCGCGCGAAAAATTAATGATCGCAAGGAAGCAGGTCAGTGAAGGCATTTCTCCTGCCGGTGAAAAGCGTAATCAAAAAGACCTTATCCGCAATGCAGATCGCTTTTCCGCTTTCGCTGAAAAATACCTTGAGGAGGTGCAACTGGCAGAAAGTACAAAAGCCCTCCGCGTGGCCACTTACGAACGAGATATTAAAGGCACGTTTGGGAATCGCTTAATGACCGAAATCACTACGGATGAAATCCGCCGCCATTGCAATAAAATTAAAGATCGCGGTGCGCCTTCCACGGCCATTTTCGTACGGGATTTAATCGCGAATATCTACCGATATGCGATTCAGCGCGGACATAAACTGCCAAATCCAGCCGATGAAATCGCAAATTCATCTATTGCAACGTTCAAAAAGCGCGAACGCGTTCTAACCCCG  >  1/1425299‑1425791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑