Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,417,660	A→G	intergenic (+258080/+3277)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,417,660	0	A	G	100.0%	182.8 / NA	69	intergenic (+258080/+3277)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/38);  total (31/38)

TTGCAATAATTCTTGCTCGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCATCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGATCGCTCATGACCGCTTCTTCCAACTCGTTTTCTTTACGCACGATCACTTTGTGACCCATTTCTTCCGC  >  1/1417420‑1417899                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                ttGCAATAATTCTTGCTCGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGttt                                                                                                                                                                                                                                       >  1:375483/1‑251 (MQ=255)   gCAATAATTCTTGCTCGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTc                                                                                                                                                                                                                                      >  1:108/1‑250 (MQ=255)    cAATAATTCTTGCTCGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCca                                                                                                                                                                                                                                    >  1:7456/1‑251 (MQ=255)      ataatTCTTGCTCGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACCCCCGCCGTTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCcaca                                                                                                                                                                                                                                  <  1:450392/251‑1 (MQ=255)          ttCTTGCTCGACGTGTTCCGGATTGGCTTCCGTTTTATCAATTTTGTTTCCTGCAACGACAATCGGCCCACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCcac                                                                                                                                                                                                                                   <  1:15940/246‑1 (MQ=255)          ttCTTGCTCGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTCCTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCcaca                                                                                                                                                                                                                                  <  1:166030/247‑1 (MQ=255)          ttCTTGCTCGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCcaca                                                                                                                                                                                                                                  <  1:257887/247‑1 (MQ=255)              tGCTCGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACg                                                                                                                                                                                                                           >  1:57412/1‑250 (MQ=255)                cTCGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCAc                                                                                                                                                                                                                        <  1:69433/251‑1 (MQ=255)                 ccGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAACCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCAcc                                                                                                                                                                                                                       <  1:674826/250‑1 (MQ=255)                    aCGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGat                                                                                                                                                                                                                    >  1:289805/1‑251 (MQ=255)                       tgtTCCGGATTGGCTTCCGTTTTATCAATTTTGTTTACTGCAACGACAATCGGCACTCCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGatat                                                                                                                                                                                                                  <  1:719059/250‑1 (MQ=255)                       tgtTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGatat                                                                                                                                                                                                                  <  1:318690/250‑1 (MQ=255)                       tgtTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATg                                                                                                                                                                                                                 <  1:19339/251‑1 (MQ=255)                        gtTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGatat                                                                                                                                                                                                                  >  1:336819/1‑249 (MQ=255)                         ttCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGt                                                                                                                                                                                                                <  1:301139/250‑1 (MQ=255)                                  ggCTTCCGTTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCAcc                                                                                                                                                                                                                       <  1:798643/234‑1 (MQ=255)                                           tttATCATTTTTGTTTACTGCAACGACAATCGGCACCCCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCtt                                                                                                                                                                                             <  1:798227/251‑1 (MQ=255)                                                            cTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCAc                                                                                                                                                                                                                        <  1:701190/207‑1 (MQ=255)                                                            cTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCAc                                                                                                                                                                                                                        <  1:686599/207‑1 (MQ=255)                                                                        tCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGAACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAata                                                                                                                                                                >  1:226670/1‑251 (MQ=255)                                                                                   cccgcTTTGGCGTGTTGGATCGCTTCGTTAGTTTGCGGCATCGCGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGAATGACCTGGCGTATCTAAGAAAGTAATCATTTTGCCGCCGTCGGTTTCCACATGGTACGCACCGATATTTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTaa                                                                                                                                                     <  1:54409/250‑1 (MQ=255)                                                                                      gcTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTaa                                                                                                                                                     >  1:511785/1‑248 (MQ=255)                                                                                              gtgtTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTg                                                                                                                                                                                                          <  1:363864/187‑1 (MQ=255)                                                                                              gtgtTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAatat                                                                                                                                                               <  1:310237/230‑1 (MQ=255)                                                                                              gtgtTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACACACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTa                                                                                                                                           >  1:425161/1‑250 (MQ=255)                                                                                               tgtTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCAc                                                                                                                                                                                                                        >  1:614327/1‑172 (MQ=255)                                                                                                 ttGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCa                                                                                                                                        <  1:156344/250‑1 (MQ=255)                                                                                                         cTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTc                                                                                                                                                           >  1:314800/1‑223 (MQ=255)                                                                                                         cTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTAc                                                                                                                                          >  1:455842/1‑240 (MQ=255)                                                                                                         cTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACg                                                                                                                               >  1:457304/1‑251 (MQ=255)                                                                                                          ttCGATAGTTTGCGGCATCCCGCCGTCATCGGCGGCAACCACAAGCACCTCGATGTCCGTCGCTTTCACACCACGCGCACGTATGAAAGTAAACGCCGCATGTCCTGGCATATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGt                                                                                                                              <  1:436099/251‑1 (MQ=255)                                                                                                                    tGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGAt                                                                                                                    <  1:538458/251‑1 (MQ=255)                                                                                                                              cGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTAc                                                                                                                                                                                                                            <  1:37782/137‑1 (MQ=255)                                                                                                                              cGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCaccacc                                                                                                          <  1:154508/251‑1 (MQ=255)                                                                                                                                ccGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCgg                                                                                                        <  1:789089/251‑1 (MQ=255)                                                                                                                                ccGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTAATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCgg                                                                                                        <  1:459302/251‑1 (MQ=255)                                                                                                                                  gTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTa                                                                                                                                                      <  1:410544/203‑1 (MQ=255)                                                                                                                                  gTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTc                                                                                                                <  1:521488/241‑1 (MQ=255)                                                                                                                                         gcggcAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCggg                                                                                                <  1:671892/250‑1 (MQ=255)                                                                                                                                            gcAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGa                                                                                             >  1:473231/1‑250 (MQ=255)                                                                                                                                                   caAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCa                                                                                                                                        >  1:700455/1‑200 (MQ=255)                                                                                                                                                      gCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCg                                                                                  >  1:56278/1‑251 (MQ=255)                                                                                                                                                           acGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAGACGCAGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCcac                                                                             <  1:54566/251‑1 (MQ=255)                                                                                                                                                                   cGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGAt                                                                     <  1:488944/251‑1 (MQ=255)                                                                                                                                                                   cGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGAt                                                                     <  1:714607/251‑1 (MQ=255)                                                                                                                                                                   cGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGAt                                                                     <  1:798091/251‑1 (MQ=255)                                                                                                                                                                        cTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGATCGCt                                                                 >  1:477929/1‑250 (MQ=255)                                                                                                                                                                        cTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTCGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTg                                                                                                      >  1:50333/1‑213 (MQ=255)                                                                                                                                                                          ttCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGAt                                                                     >  1:607216/1‑244 (MQ=255)                                                                                                                                                                                       cacgcaTGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTAc                                                                                                                                                                                                                            >  1:182593/1‑80 (MQ=255)                                                                                                                                                                                         cgcaTGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGAt                                                                                                                    <  1:255686/182‑1 (MQ=255)                                                                                                                                                                                           caTGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCggg                                                                                                >  1:890555/1‑200 (MQ=255)                                                                                                                                                                                           caTGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGATCGCTCATGACCGCTTCTTCCaa                                               >  1:281520/1‑249 (MQ=255)                                                                                                                                                                                               gAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGAt                                                                     >  1:716513/1‑223 (MQ=255)                                                                                                                                                                                               gAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGATCGCTCATGACCGCTTCTTCCAACTCGtt                                         >  1:247374/1‑251 (MQ=255)                                                                                                                                                                                                 aGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAaggagg                                                                                                                                                >  1:713219/1‑146 (MQ=255)                                                                                                                                                                                                              tGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGATCGCTCATGACCGCTTCTTCCAACt                                             <  1:148164/232‑1 (MQ=255)                                                                                                                                                                                                               gTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTAcc                                                                                                                                         >  1:564265/1‑139 (MQ=255)                                                                                                                                                                                                               gTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGAt                                                                     >  1:752582/1‑207 (MQ=255)                                                                                                                                                                                                                    ggCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGa                                                                      <  1:769842/201‑1 (MQ=255)                                                                                                                                                                                                                    ggCGTATCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGAt                                                                     <  1:144251/202‑1 (MQ=255)                                                                                                                                                                                                                         aTCTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCaccacc                                                                                                          <  1:787075/160‑1 (MQ=255)                                                                                                                                                                                                                           cTAAGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTAc                                                                                                                                          <  1:457228/126‑1 (MQ=255)                                                                                                                                                                                                                             aaGAAAGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGATCGCTCATGACCGCTTCTTCCAACTCGTTTTCTTTACGCACGATCACTTTGTGACCCAt           <  1:630245/251‑1 (MQ=255)                                                                                                                                                                                                                                  aGTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCa                                                                                                                                        >  1:525308/1‑121 (MQ=255)                                                                                                                                                                                                                                   gTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGAt                                                                                                                    >  1:891157/1‑140 (MQ=255)                                                                                                                                                                                                                                   gTGATCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCaccacc                                                                                                          >  1:92758/1‑150 (MQ=255)                                                                                                                                                                                                                                       tCATTTTGCCGTCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGATCGCTCATGACCGCTTCTTCCAACTCGTTTTCTTTACGCACGATCACTTTGTGACCCATTTCTTCCGc  >  1:594658/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                TTGCAATAATTCTTGCTCGACGTGTTCCGGATTGGCTTCCGGTTTATCAATTTTGTTTACTGCAACGACAATCGGCACACCCGCCGCTTTGGCGTGTTGGATCGCTTCGATAGTTTGCGGCATCACGCCGTCATCGGCGGCAACCACAAGCACCACGATGTCCGTCGCTTTCGCACCACGCGCACGCATGGAAGTAAACGCCGCATGTCCTGGCGTATCTAAGAAAGTGATCATTTTGCCATCGTCGGTTTCCACATGGTACGCACCGATATGTTGCGTGATCCCGCCTGCTTCGCCTGCCGCCACTTTGGCTTTACGAATATAGTCAAGTAAGGAGGTTTTACCATGGTCAACGTGCCCCATGATAGTCACCACCGGTGCGCGGGTGACCAATTCGGCGTCCACATCACGATCGCTCATGACCGCTTCTTCCAACTCGTTTTCTTTACGCACGATCACTTTGTGACCCATTTCTTCCGC  >  1/1417420‑1417899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑