Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,415,019	A→G	intergenic (+255439/+5918)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,415,019	0	A	G	100.0%	120.1 / NA	43	intergenic (+255439/+5918)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/21);  total (22/21)

AAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTACGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCATAATTTCTTTTGGAAATCTTCTAATTGTGCCAAAAACGCAATAATTTCTTGGGCAACGGTTTTTAA  >  1/1414804‑1415259                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 aaaTTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAAc                                                                                                                                                                                                               <  1:769086/251‑1 (MQ=255)         cATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAAc                                                                                                                                                                                                               <  1:893192/243‑1 (MQ=255)              aTTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCa                                                                                                                                                                                                   >  1:98628/1‑250 (MQ=255)               ttCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACg                                                                                                                                                                                                 <  1:226732/251‑1 (MQ=255)                     cTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTa                                                                                                                                                                                                                                            >  1:787506/1‑202 (MQ=255)                     cTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAAc                                                                                                                                                                                           <  1:680714/251‑1 (MQ=255)                             gTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACACAGCTTg                                                                                                                                                                                   >  1:162467/1‑251 (MQ=255)                                ttatACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCa                                                                                                                                                                                >  1:94816/1‑251 (MQ=255)                                       gcggATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGt                                                                                                                                                                                                <  1:26846/228‑1 (MQ=255)                                               atatAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCtgtg                                                                                                                                                                  >  1:154382/1‑250 (MQ=255)                                                tataAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGc                                                                                                                                                                      >  1:185751/1‑245 (MQ=255)                                                    aaTACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGAt                                                                                                                                                            <  1:246112/251‑1 (MQ=255)                                                        cATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCg                                                                                                                                                         >  1:52447/1‑250 (MQ=255)                                                             cACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGc                                                                                                                                                                                      <  1:702817/216‑1 (MQ=255)                                                             cACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGAt                                                                                                                                                            <  1:700588/242‑1 (MQ=255)                                                                     cTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGc                                                                                                                                                                      >  1:563697/1‑224 (MQ=255)                                                                     cTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGAt                                                                                                                                                            <  1:147387/234‑1 (MQ=255)                                                                        tataTCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTtcaatc                                                                                                                                        <  1:736405/251‑1 (MQ=255)                                                                                         aaaTTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAAc                                                                                                                                                                                                               >  1:224860/1‑163 (MQ=255)                                                                                          aaTTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGa                                                                                                                      <  1:661545/251‑1 (MQ=255)                                                                                           aTTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGAt                                                                                                                     <  1:293370/251‑1 (MQ=255)                                                                                                       gAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTtcaatc                                                                                                                                        <  1:829780/220‑1 (MQ=255)                                                                                                       gAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAAtt                                                                                                          <  1:604949/250‑1 (MQ=255)                                                                                                            tATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAaataa                                                                                                    <  1:853037/251‑1 (MQ=255)                                                                                                                 gaatgaatTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAAc                                                                                                                                                                                                               <  1:736657/139‑1 (MQ=255)                                                                                                                   atgaatTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAaat                                                                                                      >  1:23226/1‑242 (MQ=255)                                                                                                                       atTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTtcaatc                                                                                                                                        >  1:345065/1‑204 (MQ=255)                                                                                                                               gCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAAtt                                                                                 >  1:838539/1‑251 (MQ=255)                                                                                                                                ccATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAg                                                                                                                 <  1:381603/218‑1 (MQ=255)                                                                                                                                  aTTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAg                                                                                                                 <  1:245574/216‑1 (MQ=255)                                                                                                                                          gTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAg                                                                       >  1:897962/1‑250 (MQ=255)                                                                                                                                             cATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCAt                                                                   >  1:775434/1‑251 (MQ=255)                                                                                                                                              aTCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCATa                                                                  >  1:217921/1‑251 (MQ=255)                                                                                                                                                 tAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCATAAtt                                                               <  1:674279/251‑1 (MQ=255)                                                                                                                                                     tCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGA‑TCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCATAATTTCttt                                                          >  1:132227/1‑251 (MQ=255)                                                                                                                                                                     atatatCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGa                                                                                                                      >  1:558811/1‑176 (MQ=255)                                                                                                                                                                       atatCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGa                                                                                                                      >  1:872214/1‑174 (MQ=255)                                                                                                                                                                       atatCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAaataa                                                                                                    >  1:217229/1‑192 (MQ=255)                                                                                                                                                                       atatCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCATAATTTCTTTTGGAAATCTTCTAATtg                                         >  1:341364/1‑251 (MQ=255)                                                                                                                                                                                      cTTGGCTCAAAAAGCGACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCATAATTTCTTTTGGAAATCTTCTAATTGTGCCAAAAACGCaat                          <  1:529092/251‑1 (MQ=255)                                                                                                                                                                                                     gACGTATCCACCACCAAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCATAATTTCTTTTGGAAATCTTCTAAtt                                          <  1:867863/220‑1 (MQ=255)                                                                                                                                                                                                            ccaccaccaAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCATAATTTCTTTTGGAAATCTTCTAATTGTGCCAAAAACGCGATAATTTCTTGGGCAACGGttttt    >  1:334044/1‑251 (MQ=255)                                                                                                                                                                                                              accaccaAGTGCGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCATAATTTCTTTTGGAAATCTTCTAATTGTGCCAAAAACGCAATAATTTCTTGGGCAACGGTTTTTaa  >  1:326188/1‑251 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 AAATTTTCCATCAATTCTATCTTTTTCTGTATTATACGGCGGATCAATATAAATACATTTCACCTGTTCTTTATATCTTGCCTGCAATAAATTTAATGCTTGGAAATTATCGGAATGAATTAATAAGCCATTAGTTTGTTCATCTAGATCTTCAATGTTTTTTAATATATCTGCCTGAAAACTTGGCTCAAAAAGCGACGTATCCACCACCAAGTACGGGTAATTTTCGCAAAGTTTTTCCGCATTTAAACCGCTTGCATTCACGTTAAACAAAGCTTGCCATTGTTCTAGCTGTGCAGGATTCGCTAACGCGGTTTCAATCAATTCATCAGATAAATGATCAAGCGTAATTAAATAATGCGTGCCTGCCACAAATTTCTTTTTCAGCCATAATTTCTTTTGGAAATCTTCTAATTGTGCCAAAAACGCAATAATTTCTTGGGCAACGGTTTTTAA  >  1/1414804‑1415259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑